Campylobacter blaseri sp. nov., isolated from common seals (Phoca vitulina)

Maarten J Gilbert; Aldert L Zomer; Arjen J Timmerman; Mirlin P Spaninks; Ana Rubio García; John W A Rossen; Birgitta Duim; Jaap A Wagenaar

doi:10.1099/ijsem.0.002742

Campylobacter blaseri sp. nov., isolated from common seals (Phoca vitulina)

Maarten J Gilbert, Aldert L Zomer, Arjen J Timmerman, Mirlin P Spaninks, Ana Rubio García, John W A Rossen, Birgitta Duim, Jaap A Wagenaar

2Reptile, Amphibian and Fish Conservation Netherlands (RAVON), Nijmegen, The Netherlands.
6Wageningen Bioveterinary Research, Lelystad, The Netherlands 4WHO Collaborating Center for Campylobacter/OIE Reference Laboratory for Campylobacteriosis, Utrecht, The Netherlands 1Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
3WHO Collaborating Centre for Campylobacter/OIE Reference Laboratory for Campylobacteriosis, Utrecht, The Netherlands.
4Sealcentre, Pieterburen, The Netherlands.

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

During a study to assess the faecal microbiome of common seals (Phoca vitulina) in a Dutch seal rehabilitation centre, 16S rRNA gene sequences of an unknown Campylobactertaxon were identified. Campylobacter isolates, which differed from the established Campylobactertaxa, were cultured and their taxonomic position was determined by a polyphasic study based on ten isolates. The isolates were characterized by 16S rRNA and atpA gene sequence analyses and by conventional phenotypic testing. Based on the whole genome sequences, the average nucleotide identity and core genome phylogeny were determined. The isolates formed a separate phylogenetic clade, divergent from all other Campylobactertaxa and most closely related to Campylobacter corcagiensis, Campylobacter geochelonis and Campylobacter ureolyticus. The isolates can be distinguished phenotypically from all other Campylobactertaxa based on their lack of motility, growth at 25 °C and growth on MacConkey agar. This study shows that these isolates represent a novel species within the genus Campylobacter, for which the name Campylobacter blaseri sp. nov. is proposed. The type strain for this novel species is 17S00004-5T(=LMG 30333T=CCUG 71276T).

Original language	English
Pages (from-to)	1787-1794
Number of pages	8
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	68
Early online date	6 Apr 2018
DOIs	https://doi.org/10.1099/ijsem.0.002742
Publication status	Published - 2018

Keywords

core genome
phylogeny
average nucleotide identity
Campylobacter
common seal
microbiome
novel species

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title = "Campylobacter blaseri sp. nov., isolated from common seals (Phoca vitulina)",

abstract = "During a study to assess the faecal microbiome of common seals (Phoca vitulina) in a Dutch seal rehabilitation centre, 16S rRNA gene sequences of an unknown Campylobactertaxon were identified. Campylobacter isolates, which differed from the established Campylobactertaxa, were cultured and their taxonomic position was determined by a polyphasic study based on ten isolates. The isolates were characterized by 16S rRNA and atpA gene sequence analyses and by conventional phenotypic testing. Based on the whole genome sequences, the average nucleotide identity and core genome phylogeny were determined. The isolates formed a separate phylogenetic clade, divergent from all other Campylobactertaxa and most closely related to Campylobacter corcagiensis, Campylobacter geochelonis and Campylobacter ureolyticus. The isolates can be distinguished phenotypically from all other Campylobactertaxa based on their lack of motility, growth at 25 °C and growth on MacConkey agar. This study shows that these isolates represent a novel species within the genus Campylobacter, for which the name Campylobacter blaseri sp. nov. is proposed. The type strain for this novel species is 17S00004-5T(=LMG 30333T=CCUG 71276T).",

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author = "Gilbert, {Maarten J} and Zomer, {Aldert L} and Timmerman, {Arjen J} and Spaninks, {Mirlin P} and {Rubio Garc{\'i}a}, Ana and Rossen, {John W A} and Birgitta Duim and Wagenaar, {Jaap A}",

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AU - Gilbert, Maarten J

AU - Zomer, Aldert L

AU - Timmerman, Arjen J

AU - Spaninks, Mirlin P

AU - Rubio García, Ana

AU - Rossen, John W A

AU - Duim, Birgitta

AU - Wagenaar, Jaap A

PY - 2018

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N2 - During a study to assess the faecal microbiome of common seals (Phoca vitulina) in a Dutch seal rehabilitation centre, 16S rRNA gene sequences of an unknown Campylobactertaxon were identified. Campylobacter isolates, which differed from the established Campylobactertaxa, were cultured and their taxonomic position was determined by a polyphasic study based on ten isolates. The isolates were characterized by 16S rRNA and atpA gene sequence analyses and by conventional phenotypic testing. Based on the whole genome sequences, the average nucleotide identity and core genome phylogeny were determined. The isolates formed a separate phylogenetic clade, divergent from all other Campylobactertaxa and most closely related to Campylobacter corcagiensis, Campylobacter geochelonis and Campylobacter ureolyticus. The isolates can be distinguished phenotypically from all other Campylobactertaxa based on their lack of motility, growth at 25 °C and growth on MacConkey agar. This study shows that these isolates represent a novel species within the genus Campylobacter, for which the name Campylobacter blaseri sp. nov. is proposed. The type strain for this novel species is 17S00004-5T(=LMG 30333T=CCUG 71276T).

AB - During a study to assess the faecal microbiome of common seals (Phoca vitulina) in a Dutch seal rehabilitation centre, 16S rRNA gene sequences of an unknown Campylobactertaxon were identified. Campylobacter isolates, which differed from the established Campylobactertaxa, were cultured and their taxonomic position was determined by a polyphasic study based on ten isolates. The isolates were characterized by 16S rRNA and atpA gene sequence analyses and by conventional phenotypic testing. Based on the whole genome sequences, the average nucleotide identity and core genome phylogeny were determined. The isolates formed a separate phylogenetic clade, divergent from all other Campylobactertaxa and most closely related to Campylobacter corcagiensis, Campylobacter geochelonis and Campylobacter ureolyticus. The isolates can be distinguished phenotypically from all other Campylobactertaxa based on their lack of motility, growth at 25 °C and growth on MacConkey agar. This study shows that these isolates represent a novel species within the genus Campylobacter, for which the name Campylobacter blaseri sp. nov. is proposed. The type strain for this novel species is 17S00004-5T(=LMG 30333T=CCUG 71276T).

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KW - phylogeny

KW - average nucleotide identity

KW - Campylobacter

KW - common seal

KW - microbiome

KW - novel species

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DO - 10.1099/ijsem.0.002742

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SN - 1466-5026

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JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

ER -

Campylobacter blaseri sp. nov., isolated from common seals (Phoca vitulina)

Abstract

Keywords

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