Caenorhabditis elegans MES-3 is a highly divergent ortholog of the canonical PRC2 component SUZ12.

Berend Snel; Sander van den Heuvel; Michael F Seidl

doi:10.1016/j.isci.2022.104633

Caenorhabditis elegans MES-3 is a highly divergent ortholog of the canonical PRC2 component SUZ12.

Berend Snel, Sander van den Heuvel, Michael F Seidl

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Polycomb Repressive Complex 2 (PRC2) catalyzes the mono-, di-, and trimethylation of histone protein H3 on lysine 27 (H3K27), which is strongly associated with transcriptionally silent chromatin. The functional core of PRC2 is highly conserved in animals and consists of four subunits. One of these, SUZ12, has not been identified in the genetic model Caenorhabditis elegans, whereas C. elegans PRC2 contains the clade-specific MES-3 protein. Through unbiased sensitive sequence similarity searches complemented by high-quality structure predictions of monomers and multimers, we here demonstrate that MES-3 is a highly divergent ortholog of SUZ12. MES-3 shares protein folds and conserved residues of key domains with SUZ12 and is predicted to interact with core PRC2 members similar to SUZ12 in human PRC2. Thus, in agreement with previous genetic and biochemical studies, we provide evidence that C. elegans contains a diverged yet evolutionary conserved core PRC2, like other animals.

Original language	English
Article number	104633
Pages (from-to)	1-10
Number of pages	9
Journal	iScience
Volume	25
Issue number	7
DOIs	https://doi.org/10.1016/j.isci.2022.104633
Publication status	Published - 15 Jul 2022

Bibliographical note

Funding Information:
We would like to thank Danny Hancock for constructing the phylogenetic profiles of PRC2 core members. BS acknowledges funding via the VICI research program, project number 016.160.638, which is financed by the Dutch Research Council (NWO). SvdH contributed to this publication as part of project Single Cell Analysis of Animal Development, with project number 2019.017 of the research program OCENW GROOT/XL, which is also financed by NWO. B.S. S.v.d.H. and M.F.S. conceived the study, performed the experiments, analyzed the data, and drafted the manuscript. The authors declare no competing interests.

Funding Information:
We would like to thank Danny Hancock for constructing the phylogenetic profiles of PRC2 core members. BS acknowledges funding via the VICI research program, project number 016.160.638, which is financed by the Dutch Research Council ( NWO ). SvdH contributed to this publication as part of project Single Cell Analysis of Animal Development, with project number 2019.017 of the research program OCENW GROOT/XL, which is also financed by NWO.

Publisher Copyright:
© 2022 The Author(s)

Keywords

3days reconstruction of protein
Biochemistry
Bioinformatics
protein
sequence homology

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PIIS2589004222009051Final published version, 3.23 MBLicence: CC BY

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title = "Caenorhabditis elegans MES-3 is a highly divergent ortholog of the canonical PRC2 component SUZ12.",

abstract = "Polycomb Repressive Complex 2 (PRC2) catalyzes the mono-, di-, and trimethylation of histone protein H3 on lysine 27 (H3K27), which is strongly associated with transcriptionally silent chromatin. The functional core of PRC2 is highly conserved in animals and consists of four subunits. One of these, SUZ12, has not been identified in the genetic model Caenorhabditis elegans, whereas C. elegans PRC2 contains the clade-specific MES-3 protein. Through unbiased sensitive sequence similarity searches complemented by high-quality structure predictions of monomers and multimers, we here demonstrate that MES-3 is a highly divergent ortholog of SUZ12. MES-3 shares protein folds and conserved residues of key domains with SUZ12 and is predicted to interact with core PRC2 members similar to SUZ12 in human PRC2. Thus, in agreement with previous genetic and biochemical studies, we provide evidence that C. elegans contains a diverged yet evolutionary conserved core PRC2, like other animals. ",

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author = "Berend Snel and {van den Heuvel}, Sander and Seidl, {Michael F}",

note = "Funding Information: We would like to thank Danny Hancock for constructing the phylogenetic profiles of PRC2 core members. BS acknowledges funding via the VICI research program, project number 016.160.638, which is financed by the Dutch Research Council (NWO). SvdH contributed to this publication as part of project Single Cell Analysis of Animal Development, with project number 2019.017 of the research program OCENW GROOT/XL, which is also financed by NWO. B.S. S.v.d.H. and M.F.S. conceived the study, performed the experiments, analyzed the data, and drafted the manuscript. The authors declare no competing interests. Funding Information: We would like to thank Danny Hancock for constructing the phylogenetic profiles of PRC2 core members. BS acknowledges funding via the VICI research program, project number 016.160.638, which is financed by the Dutch Research Council ( NWO ). SvdH contributed to this publication as part of project Single Cell Analysis of Animal Development, with project number 2019.017 of the research program OCENW GROOT/XL, which is also financed by NWO. Publisher Copyright: {\textcopyright} 2022 The Author(s)",

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Caenorhabditis elegans MES-3 is a highly divergent ortholog of the canonical PRC2 component SUZ12.

Abstract

Bibliographical note

Keywords

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