Breed assignment of Italian cattle using biallelic AFLP® markers

R. Negrini; E. Milanesi; L. Colli; M. Pellecchia; L. Nicoloso; P. Crepaldi; J. A. Lenstra; P. Ajmone-Marsan

doi:10.1111/j.1365-2052.2007.01573.x

Breed assignment of Italian cattle using biallelic AFLP® markers

R. Negrini^*, E. Milanesi, L. Colli, M. Pellecchia, L. Nicoloso, P. Crepaldi, J. A. Lenstra, P. Ajmone-Marsan

^*Corresponding author for this work

Veterinary Medicine

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The verification of the breed origin of animal products is relevant for food safety and authenticity. We assessed the suitability of AFLP molecular markers in the assignment of cattle individuals to their breed of origin. Three hundred and ninety-six animals belonging to 16 cattle breeds genotyped with 141 AFLP markers were used as reference data set. Assignment was performed with likelihood (aflpop) and Bayesian (structure) methods. The Bayesian approach was superior to the likelihood algorithm with respect to (i) the correct assignment of simulated individuals to their breed of origin (93% vs. 81% respectively), (ii) the correct assignment of 44 sampled Romagnola animals (91% vs. 45% respectively) and (iii) the correct classification of animals belonging to a breed that was not included within the reference dataset. Thus, AFLP profiling in combination with the Bayesian approach seems a useful tool for breed assignment.

Original language	English
Pages (from-to)	147-153
Number of pages	7
Journal	Animal Genetics
Volume	38
Issue number	2
DOIs	https://doi.org/10.1111/j.1365-2052.2007.01573.x
Publication status	Published - 1 Apr 2007

Keywords

AFLP
Bayesian
Cattle
Likelihood
Traceability

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10.1111/j.1365-2052.2007.01573.x

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title = "Breed assignment of Italian cattle using biallelic AFLP{\textregistered} markers",

abstract = "The verification of the breed origin of animal products is relevant for food safety and authenticity. We assessed the suitability of AFLP molecular markers in the assignment of cattle individuals to their breed of origin. Three hundred and ninety-six animals belonging to 16 cattle breeds genotyped with 141 AFLP markers were used as reference data set. Assignment was performed with likelihood (aflpop) and Bayesian (structure) methods. The Bayesian approach was superior to the likelihood algorithm with respect to (i) the correct assignment of simulated individuals to their breed of origin (93% vs. 81% respectively), (ii) the correct assignment of 44 sampled Romagnola animals (91% vs. 45% respectively) and (iii) the correct classification of animals belonging to a breed that was not included within the reference dataset. Thus, AFLP profiling in combination with the Bayesian approach seems a useful tool for breed assignment.",

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AU - Milanesi, E.

AU - Colli, L.

AU - Pellecchia, M.

AU - Nicoloso, L.

AU - Crepaldi, P.

AU - Lenstra, J. A.

AU - Ajmone-Marsan, P.

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AB - The verification of the breed origin of animal products is relevant for food safety and authenticity. We assessed the suitability of AFLP molecular markers in the assignment of cattle individuals to their breed of origin. Three hundred and ninety-six animals belonging to 16 cattle breeds genotyped with 141 AFLP markers were used as reference data set. Assignment was performed with likelihood (aflpop) and Bayesian (structure) methods. The Bayesian approach was superior to the likelihood algorithm with respect to (i) the correct assignment of simulated individuals to their breed of origin (93% vs. 81% respectively), (ii) the correct assignment of 44 sampled Romagnola animals (91% vs. 45% respectively) and (iii) the correct classification of animals belonging to a breed that was not included within the reference dataset. Thus, AFLP profiling in combination with the Bayesian approach seems a useful tool for breed assignment.

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Breed assignment of Italian cattle using biallelic AFLP® markers

Abstract

Keywords

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