Biotechnology approach for conversion of agro-industrial wastes into emerging source of xylooligosaccharides

Lucas Filipe Almeida; Solimar Gonçalves Machado; Ramila Cristiane Rodrigues; Maria do Carmo Gouveia Peluzio; Tiago Antônio de Oliveira Mendes; Valéria Monteze Guimarães; Ronald de Vries; Gabriela Piccolo Maitan-Alfenas

doi:10.1007/s13399-025-06584-8

Biotechnology approach for conversion of agro-industrial wastes into emerging source of xylooligosaccharides

Lucas Filipe Almeida
, Solimar Gonçalves Machado
, Ramila Cristiane Rodrigues
, Maria do Carmo Gouveia Peluzio
, Tiago Antônio de Oliveira Mendes
, Valéria Monteze Guimarães
, Ronald de Vries
, Gabriela Piccolo Maitan-Alfenas^*

^*Corresponding author for this work

Universidade Federal de Viçosa
Fungal Physiology, Westerdijk Fungal Biodiversity Institute and Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Xylooligosaccharides (XOS) are xylose oligomers produced from agro-industrial wastes through xylanases action. They show prebiotic properties that add value to commercial products, such as beverages and foods. Soy husk, rice bran, and corn cob were pretreated and hydrolyzed by recombinant xylanases with high catalytic activity, and the XOS were quantified. Finally, XOS were evaluated for their ability to selectively stimulate the growth of the probiotic Lactobacillus acidophilus. Three recombinant xylanases were produced, with emphasis on SM2 xylanase, which showed activity of 21,853.10 U/mL. Hydrolysis of corn cob generated the higher XOS concentrations, while rice bran saccharification led to high XOS diversity. Soybean husks showed a lower yield concerning XOS release, but it stood out in generating xylohexose. The in vitro prebiotic assay showed that L. acidophilus was able to grow and to ferment the produced XOS, generating mainly propionic acid, which was not observed for the pathogens.

Original language	English
Pages (from-to)	19391-19404
Number of pages	14
Journal	Biomass Conversion and Biorefinery
Volume	15
Issue number	12
Early online date	5 Feb 2025
DOIs	https://doi.org/10.1007/s13399-025-06584-8
Publication status	Published - Jun 2025

Bibliographical note

Publisher Copyright:
© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2025.

Funding

The authors thank the Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), and Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES 001) for financial support of the work.

Funders	Funder number
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Fundação de Amparo à Pesquisa do Estado de Minas Gerais
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior	001

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

Keywords

Enzymatic hydrolysis
Lactobacillus acidophilus
Lignocellulosic biomass
Recombinant xylanases

Access to Document

10.1007/s13399-025-06584-8

Biotechnology approach for conversion of agro-industrial wastes into emerging source of xylooligosaccharidesFinal published version, 2.14 MBLicence: Taverne

Cite this

Almeida, L. F., Machado, S. G., Rodrigues, R. C., do Carmo Gouveia Peluzio, M., de Oliveira Mendes, T. A., Guimarães, V. M., de Vries, R., & Maitan-Alfenas, G. P. (2025). Biotechnology approach for conversion of agro-industrial wastes into emerging source of xylooligosaccharides. Biomass Conversion and Biorefinery , 15(12), 19391-19404. https://doi.org/10.1007/s13399-025-06584-8

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abstract = "Xylooligosaccharides (XOS) are xylose oligomers produced from agro-industrial wastes through xylanases action. They show prebiotic properties that add value to commercial products, such as beverages and foods. Soy husk, rice bran, and corn cob were pretreated and hydrolyzed by recombinant xylanases with high catalytic activity, and the XOS were quantified. Finally, XOS were evaluated for their ability to selectively stimulate the growth of the probiotic Lactobacillus acidophilus. Three recombinant xylanases were produced, with emphasis on SM2 xylanase, which showed activity of 21,853.10 U/mL. Hydrolysis of corn cob generated the higher XOS concentrations, while rice bran saccharification led to high XOS diversity. Soybean husks showed a lower yield concerning XOS release, but it stood out in generating xylohexose. The in vitro prebiotic assay showed that L. acidophilus was able to grow and to ferment the produced XOS, generating mainly propionic acid, which was not observed for the pathogens.",

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note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2025.",

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doi = "10.1007/s13399-025-06584-8",

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Almeida, LF, Machado, SG, Rodrigues, RC, do Carmo Gouveia Peluzio, M, de Oliveira Mendes, TA, Guimarães, VM, de Vries, R & Maitan-Alfenas, GP 2025, 'Biotechnology approach for conversion of agro-industrial wastes into emerging source of xylooligosaccharides', Biomass Conversion and Biorefinery , vol. 15, no. 12, pp. 19391-19404. https://doi.org/10.1007/s13399-025-06584-8

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AU - Almeida, Lucas Filipe

AU - Machado, Solimar Gonçalves

AU - Rodrigues, Ramila Cristiane

AU - do Carmo Gouveia Peluzio, Maria

AU - de Oliveira Mendes, Tiago Antônio

AU - Guimarães, Valéria Monteze

AU - de Vries, Ronald

AU - Maitan-Alfenas, Gabriela Piccolo

N1 - Publisher Copyright: © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2025.

PY - 2025/6

Y1 - 2025/6

N2 - Xylooligosaccharides (XOS) are xylose oligomers produced from agro-industrial wastes through xylanases action. They show prebiotic properties that add value to commercial products, such as beverages and foods. Soy husk, rice bran, and corn cob were pretreated and hydrolyzed by recombinant xylanases with high catalytic activity, and the XOS were quantified. Finally, XOS were evaluated for their ability to selectively stimulate the growth of the probiotic Lactobacillus acidophilus. Three recombinant xylanases were produced, with emphasis on SM2 xylanase, which showed activity of 21,853.10 U/mL. Hydrolysis of corn cob generated the higher XOS concentrations, while rice bran saccharification led to high XOS diversity. Soybean husks showed a lower yield concerning XOS release, but it stood out in generating xylohexose. The in vitro prebiotic assay showed that L. acidophilus was able to grow and to ferment the produced XOS, generating mainly propionic acid, which was not observed for the pathogens.

AB - Xylooligosaccharides (XOS) are xylose oligomers produced from agro-industrial wastes through xylanases action. They show prebiotic properties that add value to commercial products, such as beverages and foods. Soy husk, rice bran, and corn cob were pretreated and hydrolyzed by recombinant xylanases with high catalytic activity, and the XOS were quantified. Finally, XOS were evaluated for their ability to selectively stimulate the growth of the probiotic Lactobacillus acidophilus. Three recombinant xylanases were produced, with emphasis on SM2 xylanase, which showed activity of 21,853.10 U/mL. Hydrolysis of corn cob generated the higher XOS concentrations, while rice bran saccharification led to high XOS diversity. Soybean husks showed a lower yield concerning XOS release, but it stood out in generating xylohexose. The in vitro prebiotic assay showed that L. acidophilus was able to grow and to ferment the produced XOS, generating mainly propionic acid, which was not observed for the pathogens.

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KW - Lignocellulosic biomass

KW - Recombinant xylanases

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JO - Biomass Conversion and Biorefinery

JF - Biomass Conversion and Biorefinery

IS - 12

ER -

Biotechnology approach for conversion of agro-industrial wastes into emerging source of xylooligosaccharides

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

Access to Document

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