Binding of von Willebrand factor and plasma proteins to the eggshell of Schistosoma mansoni

Saskia Dewalick; Paul J Hensbergen; Michiel L Bexkens; Christina Grosserichter-Wagener; Cornelis H Hokke; André M Deelder; Philip G de Groot; Aloysius G M Tielens; Jaap J van Hellemond

doi:10.1016/j.ijpara.2013.12.006

Binding of von Willebrand factor and plasma proteins to the eggshell of Schistosoma mansoni

Saskia Dewalick, Paul J Hensbergen, Michiel L Bexkens, Christina Grosserichter-Wagener, Cornelis H Hokke, André M Deelder, Philip G de Groot, Aloysius G M Tielens, Jaap J van Hellemond

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Schistosoma mansoni eggs have to cross the endothelium and intestinal wall to leave the host and continue the life cycle. Mechanisms involved in this essential step are largely unknown. Here we describe direct binding to the S. mansoni eggshell of von Willebrand factor and other plasma proteins involved in haemostasis. Using deletion-mutants, we demonstrated that it is the A1 domain of von Willebrand factor that binds to the eggshell. Our results suggest that binding of plasma proteins to the eggshell promotes binding to the endothelium, initiating the passage of the egg through the blood-vessel wall to be excreted in the end.

Original language	English
Pages (from-to)	263-8
Number of pages	6
Journal	International Journal for Parasitology
Volume	44
Issue number	5
DOIs	https://doi.org/10.1016/j.ijpara.2013.12.006
Publication status	Published - Apr 2014

Keywords

Adult
Animals
Binding Sites
Blood Proteins
Humans
Protein Binding
Schistosoma mansoni
Sequence Deletion
Zygote
von Willebrand Factor

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title = "Binding of von Willebrand factor and plasma proteins to the eggshell of Schistosoma mansoni",

abstract = "Schistosoma mansoni eggs have to cross the endothelium and intestinal wall to leave the host and continue the life cycle. Mechanisms involved in this essential step are largely unknown. Here we describe direct binding to the S. mansoni eggshell of von Willebrand factor and other plasma proteins involved in haemostasis. Using deletion-mutants, we demonstrated that it is the A1 domain of von Willebrand factor that binds to the eggshell. Our results suggest that binding of plasma proteins to the eggshell promotes binding to the endothelium, initiating the passage of the egg through the blood-vessel wall to be excreted in the end.",

keywords = "Adult, Animals, Binding Sites, Blood Proteins, Humans, Protein Binding, Schistosoma mansoni, Sequence Deletion, Zygote, von Willebrand Factor",

author = "Saskia Dewalick and Hensbergen, {Paul J} and Bexkens, {Michiel L} and Christina Grosserichter-Wagener and Hokke, {Cornelis H} and Deelder, {Andr{\'e} M} and {de Groot}, {Philip G} and Tielens, {Aloysius G M} and {van Hellemond}, {Jaap J}",

year = "2014",

month = apr,

doi = "10.1016/j.ijpara.2013.12.006",

language = "English",

volume = "44",

pages = "263--8",

journal = "International Journal for Parasitology",

issn = "0020-7519",

publisher = "Elsevier Ltd",

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TY - JOUR

T1 - Binding of von Willebrand factor and plasma proteins to the eggshell of Schistosoma mansoni

AU - Dewalick, Saskia

AU - Hensbergen, Paul J

AU - Bexkens, Michiel L

AU - Grosserichter-Wagener, Christina

AU - Hokke, Cornelis H

AU - Deelder, André M

AU - de Groot, Philip G

AU - Tielens, Aloysius G M

AU - van Hellemond, Jaap J

PY - 2014/4

Y1 - 2014/4

N2 - Schistosoma mansoni eggs have to cross the endothelium and intestinal wall to leave the host and continue the life cycle. Mechanisms involved in this essential step are largely unknown. Here we describe direct binding to the S. mansoni eggshell of von Willebrand factor and other plasma proteins involved in haemostasis. Using deletion-mutants, we demonstrated that it is the A1 domain of von Willebrand factor that binds to the eggshell. Our results suggest that binding of plasma proteins to the eggshell promotes binding to the endothelium, initiating the passage of the egg through the blood-vessel wall to be excreted in the end.

AB - Schistosoma mansoni eggs have to cross the endothelium and intestinal wall to leave the host and continue the life cycle. Mechanisms involved in this essential step are largely unknown. Here we describe direct binding to the S. mansoni eggshell of von Willebrand factor and other plasma proteins involved in haemostasis. Using deletion-mutants, we demonstrated that it is the A1 domain of von Willebrand factor that binds to the eggshell. Our results suggest that binding of plasma proteins to the eggshell promotes binding to the endothelium, initiating the passage of the egg through the blood-vessel wall to be excreted in the end.

KW - Adult

KW - Animals

KW - Binding Sites

KW - Blood Proteins

KW - Humans

KW - Protein Binding

KW - Schistosoma mansoni

KW - Sequence Deletion

KW - Zygote

KW - von Willebrand Factor

U2 - 10.1016/j.ijpara.2013.12.006

DO - 10.1016/j.ijpara.2013.12.006

M3 - Article

C2 - 24560918

SN - 0020-7519

VL - 44

SP - 263

EP - 268

JO - International Journal for Parasitology

JF - International Journal for Parasitology

IS - 5

ER -

Binding of von Willebrand factor and plasma proteins to the eggshell of Schistosoma mansoni

Abstract

Keywords

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