Bifidobacterium breve M-16 V and scGOS/lcFOS Supplementation to Dams Ameliorates Infant Rotavirus Infection in Early Life

Laura Sáez-Fuertes; Karla Rio-Aige; Malén Massot-Cladera; Margarida Castell; Karen Knipping; Johan Garssen; Raphaëlle Bourdet-Sicard; María José Rodríguez-Lagunas; María Carmen Collado; Francisco José Pérez-Cano

doi:10.1002/mnfr.202400377

Bifidobacterium breve M-16 V and scGOS/lcFOS Supplementation to Dams Ameliorates Infant Rotavirus Infection in Early Life

Laura Sáez-Fuertes, Karla Rio-Aige, Malén Massot-Cladera, Margarida Castell, Karen Knipping, Johan Garssen, Raphaëlle Bourdet-Sicard, María José Rodríguez-Lagunas^*, María Carmen Collado, Francisco José Pérez-Cano

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The immune system of newborns is underdeveloped, leaving them susceptible to infections like rotavirus (RV). Despite vaccines, RV remains a leading cause of child mortality, especially in developing countries. Maternal immunity is transferred during pregnancy and breastfeeding to the offspring providing protection against RV infection. This study aims to explore how the maternal diet can enhance the newborn's ability to fight early infections. Pregnant rats received orally Bifidobacterium breve M-16 V and short chain galacto-oligosaccharides (scGOS)/long chain fructo-oligosaccharides (lcFOS). At day 5 of life pups are infected with RV and at day 8, samples are collected for the infection analysis. Pups whose mothers received the synbiotic have lower RV infection severity. The levels of immunoglobulins (Ig) IgG2c and IgA are raised in pups' plasma and digested milk, respectively. Synbiotic supplementation improves intestinal maturation and increases gene expression of immune-related genes. In conclusion, the administration of this synbiotic to gestating and lactating mothers ameliorates the incidence and severity of the pup's diarrhea caused by the RV infection by improving their immunity.

Original language	English
Article number	2400377
Journal	Molecular Nutrition and Food Research
Volume	68
Issue number	22
Early online date	29 Oct 2024
DOIs	https://doi.org/10.1002/mnfr.202400377
Publication status	Published - Nov 2024

Bibliographical note

Publisher Copyright:
© 2024 The Author(s). Molecular Nutrition & Food Research published by Wiley-VCH GmbH.

Funding

The research described in this paper was supported by LaMarato-TV3 (DIM-2-ELI, ref. 2018-27/30-31). The authors would like to express their gratitude to Paula Cabre for her assistance. The authors are grateful to Danone Research & Innovation for providing the synbiotic mix. INSA-UB is a Maria de Maeztu Unit of Excellence (Grant CEX2021-001234-M) and IATA-CSIC a Severo Ochoa Excellence Center (Grant CEX2021-001189-S) funded by MICIN/AEI/FEDER, UE.

Funders	Funder number
LaMarato-TV3	DIM-2-ELI, 2018-27/30-31
Maria de Maeztu Unit of Excellence	CEX2021-001234-M
IATA-CSIC a Severo Ochoa Excellence Center - MICIN/AEI/FEDER, UE	CEX2021-001189-S

Keywords

Bifidobacterium breve M-16V
infancy
long chain fructo-oligosaccharides (lcFOS)
rotavirus
short chain galacto-oligosaccharides (scGOS)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/mnfr.202400377Licence: CC BY-NC-ND

Molecular Nutrition Food Res - 2024 - Sáez‐Fuertes - Bifidobacterium breve M‐16 V and scGOS lcFOS Supplementation to DamsFinal published version, 1.39 MBLicence: CC BY-NC-ND

Cite this

Sáez-Fuertes, L., Rio-Aige, K., Massot-Cladera, M., Castell, M., Knipping, K., Garssen, J., Bourdet-Sicard, R., Rodríguez-Lagunas, M. J., Collado, M. C., & Pérez-Cano, F. J. (2024). Bifidobacterium breve M-16 V and scGOS/lcFOS Supplementation to Dams Ameliorates Infant Rotavirus Infection in Early Life. Molecular Nutrition and Food Research, 68(22), Article 2400377. https://doi.org/10.1002/mnfr.202400377

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abstract = "The immune system of newborns is underdeveloped, leaving them susceptible to infections like rotavirus (RV). Despite vaccines, RV remains a leading cause of child mortality, especially in developing countries. Maternal immunity is transferred during pregnancy and breastfeeding to the offspring providing protection against RV infection. This study aims to explore how the maternal diet can enhance the newborn's ability to fight early infections. Pregnant rats received orally Bifidobacterium breve M-16 V and short chain galacto-oligosaccharides (scGOS)/long chain fructo-oligosaccharides (lcFOS). At day 5 of life pups are infected with RV and at day 8, samples are collected for the infection analysis. Pups whose mothers received the synbiotic have lower RV infection severity. The levels of immunoglobulins (Ig) IgG2c and IgA are raised in pups' plasma and digested milk, respectively. Synbiotic supplementation improves intestinal maturation and increases gene expression of immune-related genes. In conclusion, the administration of this synbiotic to gestating and lactating mothers ameliorates the incidence and severity of the pup's diarrhea caused by the RV infection by improving their immunity.",

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Sáez-Fuertes, L, Rio-Aige, K, Massot-Cladera, M, Castell, M, Knipping, K, Garssen, J, Bourdet-Sicard, R, Rodríguez-Lagunas, MJ, Collado, MC & Pérez-Cano, FJ 2024, 'Bifidobacterium breve M-16 V and scGOS/lcFOS Supplementation to Dams Ameliorates Infant Rotavirus Infection in Early Life', Molecular Nutrition and Food Research, vol. 68, no. 22, 2400377. https://doi.org/10.1002/mnfr.202400377

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AU - Garssen, Johan

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