Band-selective editing of exchange-relay in protein-water NOE experiments

G Melacini; R Boelens; R Kaptein

doi:10.1023/A:1008350918005

Band-selective editing of exchange-relay in protein-water NOE experiments

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A pulse sequence is proposed which uses a train of band-selective pulses for the editing of slow chemical exchange-relay effects in experiments designed to study water-macromolecule interactions. Compared to previous methods, this experiment does not require knowledge of the exact chemical shift of the relaying labile protons and needs only the recording of a single experiment to edit the relay through different exchanging groups resonating at different frequencies. The pulse sequence has been implemented using Gaussian cascades and was applied to the study of the hydration of HEW lysozyme.

Original language	English
Pages (from-to)	67-71
Number of pages	5
Journal	Journal of Biomolecular NMR
Volume	13
Issue number	1
DOIs	https://doi.org/10.1023/A:1008350918005
Publication status	Published - Jan 1999

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title = "Band-selective editing of exchange-relay in protein-water NOE experiments",

abstract = "A pulse sequence is proposed which uses a train of band-selective pulses for the editing of slow chemical exchange-relay effects in experiments designed to study water-macromolecule interactions. Compared to previous methods, this experiment does not require knowledge of the exact chemical shift of the relaying labile protons and needs only the recording of a single experiment to edit the relay through different exchanging groups resonating at different frequencies. The pulse sequence has been implemented using Gaussian cascades and was applied to the study of the hydration of HEW lysozyme.",

author = "G Melacini and R Boelens and R Kaptein",

year = "1999",

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doi = "10.1023/A:1008350918005",

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AU - Boelens, R

AU - Kaptein, R

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AB - A pulse sequence is proposed which uses a train of band-selective pulses for the editing of slow chemical exchange-relay effects in experiments designed to study water-macromolecule interactions. Compared to previous methods, this experiment does not require knowledge of the exact chemical shift of the relaying labile protons and needs only the recording of a single experiment to edit the relay through different exchanging groups resonating at different frequencies. The pulse sequence has been implemented using Gaussian cascades and was applied to the study of the hydration of HEW lysozyme.

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DO - 10.1023/A:1008350918005

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SP - 67

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JO - Journal of Biomolecular NMR

JF - Journal of Biomolecular NMR

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Band-selective editing of exchange-relay in protein-water NOE experiments

Abstract

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