Bacterial Infection Disrupts Clock Gene Expression to Attenuate Immune Responses

María José de Leone; C. Esteban Hernando; Andrés Romanowski; Daniel A. Careno; Ana Faigón Soverna; Hequan Sun; Nicolás G. Bologna; Martín Vázquez; Korbinian Schneeberger; Marcelo J. Yanovsky

doi:10.1016/j.cub.2020.02.058

Bacterial Infection Disrupts Clock Gene Expression to Attenuate Immune Responses

María José de Leone
, C. Esteban Hernando
, Andrés Romanowski
, Daniel A. Careno
, Ana Faigón Soverna
, Hequan Sun
, Nicolás G. Bologna
, Martín Vázquez
, Korbinian Schneeberger
, Marcelo J. Yanovsky^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Little is known regarding the mechanisms involved in the clock-immunity crosstalk. de Leone et al. report that the defense mutant eds4 carries a mutation in NUCLEOPORIN205 which affects its circadian function, and that leads to an attenuation of the transcriptional reprogramming in core clock genes that take place soon after a bacterial infection.

Original language	English
Pages (from-to)	1740-1747.e6
Journal	Current Biology
Volume	30
Issue number	9
DOIs	https://doi.org/10.1016/j.cub.2020.02.058
Publication status	Published - 4 May 2020
Externally published	Yes

Bibliographical note

Funding Information:
We thank J. Hildebrandt, D. Lundberg, E. Symeonidi, and the Weigel laboratory at the Max Planck Institute for Developmental Biology in Tübingen for technical assistance in the DNA sequencing; J. Mateos and S. Mora-García for helpful discussion on the project; and P. Cerdán for suggestions for the mapping process. This work was supported by grants from the Agencia Nacional de Promoción Científica y Tecnológica of Argentina (to M.J.Y.).

Funding Information:
We thank J. Hildebrandt, D. Lundberg, E. Symeonidi, and the Weigel laboratory at the Max Planck Institute for Developmental Biology in T?bingen for technical assistance in the DNA sequencing; J. Mateos and S. Mora-Garc?a for helpful discussion on the project; and P. Cerd?n for suggestions for the mapping process. This work was supported by grants from the Agencia Nacional de Promoci?n Cient?fica y Tecnol?gica of Argentina (to M.J.Y.). M.J.d.L. C.E.H. D.A.C. and A.F.S. performed the experiments and statistical analysis. A.R. obtained LNK quadruple mutant. C.E.H. constructed the complementary DNA (cDNA) libraries and performed the bioinformatic analysis of the RNAseq data. M.V. performed the RNA-seq protocols. H.S. executed and K.S. supervised SHOREmapping. N.G.B. performed the nuclear and total RNA extraction. M.J.d.L. and M.J.Y. wrote the paper. M.J.Y. supervised the project. All authors discussed the results and commented on the manuscript. The authors declare no competing interests.

Publisher Copyright:
© 2020 Elsevier Inc.

Funding

We thank J. Hildebrandt, D. Lundberg, E. Symeonidi, and the Weigel laboratory at the Max Planck Institute for Developmental Biology in Tübingen for technical assistance in the DNA sequencing; J. Mateos and S. Mora-García for helpful discussion on the project; and P. Cerdán for suggestions for the mapping process. This work was supported by grants from the Agencia Nacional de Promoción Científica y Tecnológica of Argentina (to M.J.Y.). We thank J. Hildebrandt, D. Lundberg, E. Symeonidi, and the Weigel laboratory at the Max Planck Institute for Developmental Biology in T?bingen for technical assistance in the DNA sequencing; J. Mateos and S. Mora-Garc?a for helpful discussion on the project; and P. Cerd?n for suggestions for the mapping process. This work was supported by grants from the Agencia Nacional de Promoci?n Cient?fica y Tecnol?gica of Argentina (to M.J.Y.). M.J.d.L. C.E.H. D.A.C. and A.F.S. performed the experiments and statistical analysis. A.R. obtained LNK quadruple mutant. C.E.H. constructed the complementary DNA (cDNA) libraries and performed the bioinformatic analysis of the RNAseq data. M.V. performed the RNA-seq protocols. H.S. executed and K.S. supervised SHOREmapping. N.G.B. performed the nuclear and total RNA extraction. M.J.d.L. and M.J.Y. wrote the paper. M.J.Y. supervised the project. All authors discussed the results and commented on the manuscript. The authors declare no competing interests.

Keywords

eds4, circadian clock
Enhanced Disease Susceptibility 4
LNK
NIGHT LIGHT-INDUCIBLE AND CLOCK-REGULATED
NPC
Nuclear Pore Complex
plant-pathogen interactions

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PIIS0960982220302657Final published version, 2.82 MB

Cite this

@article{d5a5516f28c94a9b86b8747bffd9d3ed,

title = "Bacterial Infection Disrupts Clock Gene Expression to Attenuate Immune Responses",

abstract = "Little is known regarding the mechanisms involved in the clock-immunity crosstalk. de Leone et al. report that the defense mutant eds4 carries a mutation in NUCLEOPORIN205 which affects its circadian function, and that leads to an attenuation of the transcriptional reprogramming in core clock genes that take place soon after a bacterial infection.",

keywords = "eds4, circadian clock, Enhanced Disease Susceptibility 4, LNK, NIGHT LIGHT-INDUCIBLE AND CLOCK-REGULATED, NPC, Nuclear Pore Complex, plant-pathogen interactions",

author = "\{de Leone\}, \{Mar{\'i}a Jos{\'e}\} and Hernando, \{C. Esteban\} and Andr{\'e}s Romanowski and Careno, \{Daniel A.\} and Soverna, \{Ana Faig{\'o}n\} and Hequan Sun and Bologna, \{Nicol{\'a}s G.\} and Mart{\'i}n V{\'a}zquez and Korbinian Schneeberger and Yanovsky, \{Marcelo J.\}",

note = "Funding Information: We thank J. Hildebrandt, D. Lundberg, E. Symeonidi, and the Weigel laboratory at the Max Planck Institute for Developmental Biology in T{\"u}bingen for technical assistance in the DNA sequencing; J. Mateos and S. Mora-Garc{\'i}a for helpful discussion on the project; and P. Cerd{\'a}n for suggestions for the mapping process. This work was supported by grants from the Agencia Nacional de Promoci{\'o}n Cient{\'i}fica y Tecnol{\'o}gica of Argentina (to M.J.Y.). Funding Information: We thank J. Hildebrandt, D. Lundberg, E. Symeonidi, and the Weigel laboratory at the Max Planck Institute for Developmental Biology in T?bingen for technical assistance in the DNA sequencing; J. Mateos and S. Mora-Garc?a for helpful discussion on the project; and P. Cerd?n for suggestions for the mapping process. This work was supported by grants from the Agencia Nacional de Promoci?n Cient?fica y Tecnol?gica of Argentina (to M.J.Y.). M.J.d.L. C.E.H. D.A.C. and A.F.S. performed the experiments and statistical analysis. A.R. obtained LNK quadruple mutant. C.E.H. constructed the complementary DNA (cDNA) libraries and performed the bioinformatic analysis of the RNAseq data. M.V. performed the RNA-seq protocols. H.S. executed and K.S. supervised SHOREmapping. N.G.B. performed the nuclear and total RNA extraction. M.J.d.L. and M.J.Y. wrote the paper. M.J.Y. supervised the project. All authors discussed the results and commented on the manuscript. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

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doi = "10.1016/j.cub.2020.02.058",

language = "English",

volume = "30",

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journal = "Current Biology",

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T1 - Bacterial Infection Disrupts Clock Gene Expression to Attenuate Immune Responses

AU - de Leone, María José

AU - Hernando, C. Esteban

AU - Romanowski, Andrés

AU - Careno, Daniel A.

AU - Soverna, Ana Faigón

AU - Sun, Hequan

AU - Bologna, Nicolás G.

AU - Vázquez, Martín

AU - Schneeberger, Korbinian

AU - Yanovsky, Marcelo J.

N1 - Funding Information: We thank J. Hildebrandt, D. Lundberg, E. Symeonidi, and the Weigel laboratory at the Max Planck Institute for Developmental Biology in Tübingen for technical assistance in the DNA sequencing; J. Mateos and S. Mora-García for helpful discussion on the project; and P. Cerdán for suggestions for the mapping process. This work was supported by grants from the Agencia Nacional de Promoción Científica y Tecnológica of Argentina (to M.J.Y.). Funding Information: We thank J. Hildebrandt, D. Lundberg, E. Symeonidi, and the Weigel laboratory at the Max Planck Institute for Developmental Biology in T?bingen for technical assistance in the DNA sequencing; J. Mateos and S. Mora-Garc?a for helpful discussion on the project; and P. Cerd?n for suggestions for the mapping process. This work was supported by grants from the Agencia Nacional de Promoci?n Cient?fica y Tecnol?gica of Argentina (to M.J.Y.). M.J.d.L. C.E.H. D.A.C. and A.F.S. performed the experiments and statistical analysis. A.R. obtained LNK quadruple mutant. C.E.H. constructed the complementary DNA (cDNA) libraries and performed the bioinformatic analysis of the RNAseq data. M.V. performed the RNA-seq protocols. H.S. executed and K.S. supervised SHOREmapping. N.G.B. performed the nuclear and total RNA extraction. M.J.d.L. and M.J.Y. wrote the paper. M.J.Y. supervised the project. All authors discussed the results and commented on the manuscript. The authors declare no competing interests. Publisher Copyright: © 2020 Elsevier Inc.

PY - 2020/5/4

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N2 - Little is known regarding the mechanisms involved in the clock-immunity crosstalk. de Leone et al. report that the defense mutant eds4 carries a mutation in NUCLEOPORIN205 which affects its circadian function, and that leads to an attenuation of the transcriptional reprogramming in core clock genes that take place soon after a bacterial infection.

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KW - eds4, circadian clock

KW - Enhanced Disease Susceptibility 4

KW - LNK

KW - NIGHT LIGHT-INDUCIBLE AND CLOCK-REGULATED

KW - NPC

KW - Nuclear Pore Complex

KW - plant-pathogen interactions

UR - https://www.scopus.com/pages/publications/85083017098

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SN - 0960-9822

VL - 30

SP - 1740-1747.e6

JO - Current Biology

JF - Current Biology

IS - 9

ER -

Bacterial Infection Disrupts Clock Gene Expression to Attenuate Immune Responses

Abstract

Bibliographical note

Funding

Keywords

Access to Document

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