Aurora B controls microtubule stability to regulate abscission dynamics in stem cells

Snježana Kodba; Amber Öztop; Eri van Berkum; Eugene A. Katrukha; Malina K. Iwanski; Wilco Nijenhuis; Lukas C. Kapitein; Agathe Chaigne

doi:10.1016/j.celrep.2025.115238

Aurora B controls microtubule stability to regulate abscission dynamics in stem cells

Snježana Kodba
, Amber Öztop
, Eri van Berkum
, Eugene A. Katrukha
, Malina K. Iwanski
, Wilco Nijenhuis
, Lukas C. Kapitein
, Agathe Chaigne^*

^*Corresponding author for this work

Utrecht University

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Abscission is the last step of cell division. It separates the two sister cells and consists of cutting the cytoplasmic bridge. Abscission is mediated by the ESCRT membrane remodeling machinery, which also triggers the severing of a thick bundle of microtubules. Here, we show that rather than being passive actors in abscission, microtubules control abscission speed. Using mouse embryonic stem cells, which transition from slow to fast abscission during exit from naive pluripotency, we investigate the molecular mechanism for the regulation of abscission dynamics and identify crosstalk between Aurora B activity and microtubule stability. We demonstrate that naive stem cells maintain high Aurora B activity on the bridge after cytokinesis. This high Aurora B activity leads to transient microtubule stabilization that delays abscission by decreasing MCAK recruitment to the midbody. In turn, stable microtubules promote the activity of Aurora B. Overall, our data demonstrate that Aurora B-dependent microtubule stability controls abscission dynamics.

Original language	English
Article number	115238
Journal	Cell Reports
Volume	44
Issue number	2
DOIs	https://doi.org/10.1016/j.celrep.2025.115238
Publication status	Published - 25 Feb 2025

Bibliographical note

Publisher Copyright:
© 2025 The Authors

Funding

This work was supported by Utrecht University and NWO (NWO-Vidi to A.C.), the European Research Council (ERC Consolidator Grant 819219 to L.C.K.), and the Centre for Living Technologies, a part of the Alliance TU/e, WUR, UU, and UMC Utrecht (Eindhoven University of Technology, Wageningen University & Research, Utrecht University, and University Medical Center Utrecht; www.ewuu.nl).

Funders	Funder number
Nederlandse Organisatie voor Wetenschappelijk Onderzoek
Centre for Living Technologies
Universitair Medisch Centrum Utrecht
Universiteit Utrecht
University Medical Center Utrecht
European Research Council	819219

Keywords

abscission
Aurora B
CP: Cell biology
CP: Stem cell research
cytoplasmic bridges
MCAK
microtubules
stem cells

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Cite this

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title = "Aurora B controls microtubule stability to regulate abscission dynamics in stem cells",

abstract = "Abscission is the last step of cell division. It separates the two sister cells and consists of cutting the cytoplasmic bridge. Abscission is mediated by the ESCRT membrane remodeling machinery, which also triggers the severing of a thick bundle of microtubules. Here, we show that rather than being passive actors in abscission, microtubules control abscission speed. Using mouse embryonic stem cells, which transition from slow to fast abscission during exit from naive pluripotency, we investigate the molecular mechanism for the regulation of abscission dynamics and identify crosstalk between Aurora B activity and microtubule stability. We demonstrate that naive stem cells maintain high Aurora B activity on the bridge after cytokinesis. This high Aurora B activity leads to transient microtubule stabilization that delays abscission by decreasing MCAK recruitment to the midbody. In turn, stable microtubules promote the activity of Aurora B. Overall, our data demonstrate that Aurora B-dependent microtubule stability controls abscission dynamics.",

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author = "Snje{\v z}ana Kodba and Amber {\"O}ztop and \{van Berkum\}, Eri and Katrukha, \{Eugene A.\} and Iwanski, \{Malina K.\} and Wilco Nijenhuis and Kapitein, \{Lukas C.\} and Agathe Chaigne",

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doi = "10.1016/j.celrep.2025.115238",

language = "English",

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T1 - Aurora B controls microtubule stability to regulate abscission dynamics in stem cells

AU - Kodba, Snježana

AU - Öztop, Amber

AU - van Berkum, Eri

AU - Katrukha, Eugene A.

AU - Iwanski, Malina K.

AU - Nijenhuis, Wilco

AU - Kapitein, Lukas C.

AU - Chaigne, Agathe

PY - 2025/2/25

Y1 - 2025/2/25

N2 - Abscission is the last step of cell division. It separates the two sister cells and consists of cutting the cytoplasmic bridge. Abscission is mediated by the ESCRT membrane remodeling machinery, which also triggers the severing of a thick bundle of microtubules. Here, we show that rather than being passive actors in abscission, microtubules control abscission speed. Using mouse embryonic stem cells, which transition from slow to fast abscission during exit from naive pluripotency, we investigate the molecular mechanism for the regulation of abscission dynamics and identify crosstalk between Aurora B activity and microtubule stability. We demonstrate that naive stem cells maintain high Aurora B activity on the bridge after cytokinesis. This high Aurora B activity leads to transient microtubule stabilization that delays abscission by decreasing MCAK recruitment to the midbody. In turn, stable microtubules promote the activity of Aurora B. Overall, our data demonstrate that Aurora B-dependent microtubule stability controls abscission dynamics.

AB - Abscission is the last step of cell division. It separates the two sister cells and consists of cutting the cytoplasmic bridge. Abscission is mediated by the ESCRT membrane remodeling machinery, which also triggers the severing of a thick bundle of microtubules. Here, we show that rather than being passive actors in abscission, microtubules control abscission speed. Using mouse embryonic stem cells, which transition from slow to fast abscission during exit from naive pluripotency, we investigate the molecular mechanism for the regulation of abscission dynamics and identify crosstalk between Aurora B activity and microtubule stability. We demonstrate that naive stem cells maintain high Aurora B activity on the bridge after cytokinesis. This high Aurora B activity leads to transient microtubule stabilization that delays abscission by decreasing MCAK recruitment to the midbody. In turn, stable microtubules promote the activity of Aurora B. Overall, our data demonstrate that Aurora B-dependent microtubule stability controls abscission dynamics.

KW - abscission

KW - Aurora B

KW - CP: Cell biology

KW - CP: Stem cell research

KW - cytoplasmic bridges

KW - MCAK

KW - microtubules

KW - stem cells

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DO - 10.1016/j.celrep.2025.115238

M3 - Article

AN - SCOPUS:85215628682

SN - 2639-1856

VL - 44

JO - Cell Reports

JF - Cell Reports

IS - 2

M1 - 115238

ER -

Aurora B controls microtubule stability to regulate abscission dynamics in stem cells

Abstract

Bibliographical note

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Keywords

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