Asthmatic farm children show increased CD3+CD8low T-cells compared to non-asthmatic farm children

Monika Twardziok; Paul C Schröder; Johanna Krusche; Vera I Casaca; Sabina Illi; Andreas Böck; Georg J Loss; Michael Kabesch; Antoaneta A Toncheva; Caroline Roduit; Martin Depner; Jon Genuneit; Harald Renz; Marjut Roponen; Juliane Weber; Charlotte Braun-Fahrländer; Josef Riedler; Roger P. Lauener; Dominique Angèle Vuitton; Jean-Charles Dalphin; Juha Pekkanen; Erika von Mutius; Bianca Schaub; Anne Hyvärinen; Anne M Karvonen; Pirkka V Kirjavainen; Sami Remes; Vincent Kaulek; Marie-Laure Dalphin; Markus Johannes Ege; Petra Ina Pfefferle; Gert Doekes; PASTURE Study Group:

doi:10.1016/j.clim.2017.09.009

Asthmatic farm children show increased CD3+CD8low T-cells compared to non-asthmatic farm children

Monika Twardziok, Paul C Schröder, Johanna Krusche, Vera I Casaca, Sabina Illi, Andreas Böck, Georg J Loss, Michael Kabesch, Antoaneta A Toncheva, Caroline Roduit, Martin Depner, Jon Genuneit, Harald Renz, Marjut Roponen, Juliane Weber, Charlotte Braun-Fahrländer, Josef Riedler, Roger P. Lauener, Dominique Angèle Vuitton, Jean-Charles DalphinJuha Pekkanen, Erika von Mutius, Bianca Schaub, Anne Hyvärinen, Anne M Karvonen, Pirkka V Kirjavainen, Sami Remes, Vincent Kaulek, Marie-Laure Dalphin, Markus Johannes Ege, Petra Ina Pfefferle, Gert Doekes, PASTURE Study Group:

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Several studies report an important role of CD8+ cytotoxic T-cells in atopy. Farm children show protection against
atopy development, partly explained by CD4+ T-cell subtypes. Additional effects of CD8+ T-cells are unknown
being investigated in this study within the PASTURE/EFRAIM birth cohort in PBMCs from farming and non-farming
6-year-old (N=76) German children. CD3+CD8+CD25+ T-cells were analyzed by flow cytometry. Genotyping of
17q21 locus-SNPs associated with childhood asthma was performed. No differences in CD8+ T-cell subsets were
seen between farmers and non-farmers regardless of asthma. Among farmchildren, asthmatics displayed increased
CD3+CD8low(CD25+) T-cells compared to non-asthmatics. Asthmatic farm children exhibited a lower PI-induced
stimulatory capacity of CD3+CD8low(CD25+) cells and a lower IFN-γ secretion than non-asthmatic farm children.
Among farm children with GSDMB and ORMDL3 risk alleles, asthmatics displayed higher CD3+CD8low cells than
non-asthmatics. Our data indicates a specific role of CD8low T-cells in asthmatic farm children.

Original language	English
Pages (from-to)	285-292
Number of pages	8
Journal	Clinical Immunology
Volume	183
DOIs	https://doi.org/10.1016/j.clim.2017.09.009
Publication status	Published - Oct 2017

Keywords

CD8=+
CD8low
Childhood
farm-exposure

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10.1016/j.clim.2017.09.009

1-s2.0-S1521661617303017-mainFinal published version, 699 KBLicence: Taverne

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Twardziok, M., Schröder, P. C., Krusche, J., Casaca, V. I., Illi, S., Böck, A., Loss, G. J., Kabesch, M., Toncheva, A. A., Roduit, C., Depner, M., Genuneit, J., Renz, H., Roponen, M., Weber, J., Braun-Fahrländer, C., Riedler, J., Lauener, R. P., Vuitton, D. A., ... PASTURE Study Group: (2017). Asthmatic farm children show increased CD3+CD8low T-cells compared to non-asthmatic farm children. Clinical Immunology, 183, 285-292. https://doi.org/10.1016/j.clim.2017.09.009

@article{5e058ebbd2c34ab9a66f49f7554b88b2,

title = "Asthmatic farm children show increased CD3+CD8low T-cells compared to non-asthmatic farm children",

abstract = "Several studies report an important role of CD8+ cytotoxic T-cells in atopy. Farm children show protection against atopy development, partly explained by CD4+ T-cell subtypes. Additional effects of CD8+ T-cells are unknown being investigated in this study within the PASTURE/EFRAIM birth cohort in PBMCs from farming and non-farming 6-year-old (N=76) German children. CD3+CD8+CD25+ T-cells were analyzed by flow cytometry. Genotyping of 17q21 locus-SNPs associated with childhood asthma was performed. No differences in CD8+ T-cell subsets were seen between farmers and non-farmers regardless of asthma. Among farmchildren, asthmatics displayed increased CD3+CD8low(CD25+) T-cells compared to non-asthmatics. Asthmatic farm children exhibited a lower PI-induced stimulatory capacity of CD3+CD8low(CD25+) cells and a lower IFN-γ secretion than non-asthmatic farm children. Among farm children with GSDMB and ORMDL3 risk alleles, asthmatics displayed higher CD3+CD8low cells than non-asthmatics. Our data indicates a specific role of CD8low T-cells in asthmatic farm children.",

keywords = "CD8=+, CD8low, Childhood, farm-exposure",

author = "Monika Twardziok and Schr{\"o}der, \{Paul C\} and Johanna Krusche and Casaca, \{Vera I\} and Sabina Illi and Andreas B{\"o}ck and Loss, \{Georg J\} and Michael Kabesch and Toncheva, \{Antoaneta A\} and Caroline Roduit and Martin Depner and Jon Genuneit and Harald Renz and Marjut Roponen and Juliane Weber and Charlotte Braun-Fahrl{\"a}nder and Josef Riedler and Lauener, \{Roger P.\} and Vuitton, \{Dominique Ang{\`e}le\} and Jean-Charles Dalphin and Juha Pekkanen and \{von Mutius\}, Erika and Bianca Schaub and Anne Hyv{\"a}rinen and Karvonen, \{Anne M\} and Kirjavainen, \{Pirkka V\} and Sami Remes and Vincent Kaulek and Marie-Laure Dalphin and Ege, \{Markus Johannes\} and Pfefferle, \{Petra Ina\} and Gert Doekes and \{PASTURE Study Group:\}",

year = "2017",

month = oct,

doi = "10.1016/j.clim.2017.09.009",

language = "English",

volume = "183",

pages = "285--292",

journal = "Clinical Immunology",

issn = "1521-6616",

publisher = "Academic Press Inc.",

}

Twardziok, M, Schröder, PC, Krusche, J, Casaca, VI, Illi, S, Böck, A, Loss, GJ, Kabesch, M, Toncheva, AA, Roduit, C, Depner, M, Genuneit, J, Renz, H, Roponen, M, Weber, J, Braun-Fahrländer, C, Riedler, J, Lauener, RP, Vuitton, DA, Dalphin, J-C, Pekkanen, J, von Mutius, E, Schaub, B, Hyvärinen, A, Karvonen, AM, Kirjavainen, PV, Remes, S, Kaulek, V, Dalphin, M-L, Ege, MJ, Pfefferle, PI, Doekes, G & PASTURE Study Group: 2017, 'Asthmatic farm children show increased CD3+CD8low T-cells compared to non-asthmatic farm children', Clinical Immunology, vol. 183, pp. 285-292. https://doi.org/10.1016/j.clim.2017.09.009

TY - JOUR

T1 - Asthmatic farm children show increased CD3+CD8low T-cells compared to non-asthmatic farm children

AU - Twardziok, Monika

AU - Schröder, Paul C

AU - Krusche, Johanna

AU - Casaca, Vera I

AU - Illi, Sabina

AU - Böck, Andreas

AU - Loss, Georg J

AU - Kabesch, Michael

AU - Toncheva, Antoaneta A

AU - Roduit, Caroline

AU - Depner, Martin

AU - Genuneit, Jon

AU - Renz, Harald

AU - Roponen, Marjut

AU - Weber, Juliane

AU - Braun-Fahrländer, Charlotte

AU - Riedler, Josef

AU - Lauener, Roger P.

AU - Vuitton, Dominique Angèle

AU - Dalphin, Jean-Charles

AU - Pekkanen, Juha

AU - von Mutius, Erika

AU - Schaub, Bianca

AU - Hyvärinen, Anne

AU - Karvonen, Anne M

AU - Kirjavainen, Pirkka V

AU - Remes, Sami

AU - Kaulek, Vincent

AU - Dalphin, Marie-Laure

AU - Ege, Markus Johannes

AU - Pfefferle, Petra Ina

AU - Doekes, Gert

AU - PASTURE Study Group:

PY - 2017/10

Y1 - 2017/10

N2 - Several studies report an important role of CD8+ cytotoxic T-cells in atopy. Farm children show protection against atopy development, partly explained by CD4+ T-cell subtypes. Additional effects of CD8+ T-cells are unknown being investigated in this study within the PASTURE/EFRAIM birth cohort in PBMCs from farming and non-farming 6-year-old (N=76) German children. CD3+CD8+CD25+ T-cells were analyzed by flow cytometry. Genotyping of 17q21 locus-SNPs associated with childhood asthma was performed. No differences in CD8+ T-cell subsets were seen between farmers and non-farmers regardless of asthma. Among farmchildren, asthmatics displayed increased CD3+CD8low(CD25+) T-cells compared to non-asthmatics. Asthmatic farm children exhibited a lower PI-induced stimulatory capacity of CD3+CD8low(CD25+) cells and a lower IFN-γ secretion than non-asthmatic farm children. Among farm children with GSDMB and ORMDL3 risk alleles, asthmatics displayed higher CD3+CD8low cells than non-asthmatics. Our data indicates a specific role of CD8low T-cells in asthmatic farm children.

AB - Several studies report an important role of CD8+ cytotoxic T-cells in atopy. Farm children show protection against atopy development, partly explained by CD4+ T-cell subtypes. Additional effects of CD8+ T-cells are unknown being investigated in this study within the PASTURE/EFRAIM birth cohort in PBMCs from farming and non-farming 6-year-old (N=76) German children. CD3+CD8+CD25+ T-cells were analyzed by flow cytometry. Genotyping of 17q21 locus-SNPs associated with childhood asthma was performed. No differences in CD8+ T-cell subsets were seen between farmers and non-farmers regardless of asthma. Among farmchildren, asthmatics displayed increased CD3+CD8low(CD25+) T-cells compared to non-asthmatics. Asthmatic farm children exhibited a lower PI-induced stimulatory capacity of CD3+CD8low(CD25+) cells and a lower IFN-γ secretion than non-asthmatic farm children. Among farm children with GSDMB and ORMDL3 risk alleles, asthmatics displayed higher CD3+CD8low cells than non-asthmatics. Our data indicates a specific role of CD8low T-cells in asthmatic farm children.

KW - CD8=+

KW - CD8low

KW - Childhood

KW - farm-exposure

U2 - 10.1016/j.clim.2017.09.009

DO - 10.1016/j.clim.2017.09.009

M3 - Article

C2 - 28917722

SN - 1521-6616

VL - 183

SP - 285

EP - 292

JO - Clinical Immunology

JF - Clinical Immunology

ER -

Asthmatic farm children show increased CD3+CD8low T-cells compared to non-asthmatic farm children

Abstract

Keywords

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