Association of circulating microRNA-122 and microRNA-29a with stage of fibrosis and progression of chronic hepatitis in Labrador Retrievers

Manabu Sakai; Bart Spee; Guy C M Grinwis; Louis C Penning; Monique E van Wolferen; Luc J W van der Laan; Hille Fieten

doi:10.1111/jvim.15366

Association of circulating microRNA-122 and microRNA-29a with stage of fibrosis and progression of chronic hepatitis in Labrador Retrievers

Manabu Sakai, Bart Spee, Guy C M Grinwis, Louis C Penning, Monique E van Wolferen, Luc J W van der Laan, Hille Fieten

Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Kanagawa, Japan.
Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 108, 3584 CM, Utrecht, The Netherlands. Electronic address: [email protected].
Utrecht University
Erasmus University Medical Center Rotterdam

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: Chronic hepatitis (CH) in dogs is common and has the tendency to progress to liver cirrhosis (LC). Circulating microRNAs might have the potential as markers for disease progression.

OBJECTIVES: To investigate whether concentration of specific microRNAs in serum correlate with the stage and grade of CH in Labrador Retrievers.

ANIMALS: Twenty-two Labrador Retrievers with histological CH (n = 8), LC (n = 7), and normal liver (NL, n = 7).

METHODS: In this retrospective study, serum concentrations of miR-122, miR-29a, miR-133a, miR-181b, and miR-17-5p were measured by quantitative real-time PCR and evaluated using univariate linear regression in dogs. A multivariate model was fit including the grade of hepatitis and the stage of fibrosis.

RESULTS: Of the 5 microRNAs, only circulating miR-122 and miR-29a were significantly associated with the grade of hepatitis and the stage of fibrosis. A positive correlation was identified between the grade of hepatitis with miR-122 (rs = 0.79, P < .001) and miR-29a (rs = 0.78, P < .001). Both miR-122 (rs = 0.81, P < .001) and miR-29a (rs = 0.67, P < .001) showed a significant positive correlation with the stage of fibrosis. MiR-122 concentrations were significantly higher in the CH (P < .01) and LC groups (P < .001) compared to the NL group. MiR-29a concentrations were significantly higher in the CH (P < .001) and LC (P < .001) groups compared to the NL group.

CONCLUSIONS AND CLINICAL IMPORTANCE: Circulating miR-122 and miR-29a concentrations might be useful for monitoring the response to treatment and progression of canine CH.

Original language	English
Pages (from-to)	151-157
Journal	Journal of Veterinary Internal Medicine
Volume	33
Issue number	1
DOIs	https://doi.org/10.1111/jvim.15366
Publication status	Published - Jan 2019

Keywords

canine
cirrhosis
dog
fibrosis
liver injury

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/jvim.15366

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title = "Association of circulating microRNA-122 and microRNA-29a with stage of fibrosis and progression of chronic hepatitis in Labrador Retrievers",

abstract = "BACKGROUND: Chronic hepatitis (CH) in dogs is common and has the tendency to progress to liver cirrhosis (LC). Circulating microRNAs might have the potential as markers for disease progression.OBJECTIVES: To investigate whether concentration of specific microRNAs in serum correlate with the stage and grade of CH in Labrador Retrievers.ANIMALS: Twenty-two Labrador Retrievers with histological CH (n = 8), LC (n = 7), and normal liver (NL, n = 7).METHODS: In this retrospective study, serum concentrations of miR-122, miR-29a, miR-133a, miR-181b, and miR-17-5p were measured by quantitative real-time PCR and evaluated using univariate linear regression in dogs. A multivariate model was fit including the grade of hepatitis and the stage of fibrosis.RESULTS: Of the 5 microRNAs, only circulating miR-122 and miR-29a were significantly associated with the grade of hepatitis and the stage of fibrosis. A positive correlation was identified between the grade of hepatitis with miR-122 (rs = 0.79, P < .001) and miR-29a (rs = 0.78, P < .001). Both miR-122 (rs = 0.81, P < .001) and miR-29a (rs = 0.67, P < .001) showed a significant positive correlation with the stage of fibrosis. MiR-122 concentrations were significantly higher in the CH (P < .01) and LC groups (P < .001) compared to the NL group. MiR-29a concentrations were significantly higher in the CH (P < .001) and LC (P < .001) groups compared to the NL group.CONCLUSIONS AND CLINICAL IMPORTANCE: Circulating miR-122 and miR-29a concentrations might be useful for monitoring the response to treatment and progression of canine CH.",

keywords = "canine, cirrhosis, dog, fibrosis, liver injury",

author = "Manabu Sakai and Bart Spee and Grinwis, {Guy C M} and Penning, {Louis C} and {van Wolferen}, {Monique E} and {van der Laan}, {Luc J W} and Hille Fieten",

note = "{\textcopyright} 2018 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.",

year = "2019",

month = jan,

doi = "10.1111/jvim.15366",

language = "English",

volume = "33",

pages = "151--157",

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T1 - Association of circulating microRNA-122 and microRNA-29a with stage of fibrosis and progression of chronic hepatitis in Labrador Retrievers

AU - Sakai, Manabu

AU - Spee, Bart

AU - Grinwis, Guy C M

AU - Penning, Louis C

AU - van Wolferen, Monique E

AU - van der Laan, Luc J W

AU - Fieten, Hille

PY - 2019/1

Y1 - 2019/1

N2 - BACKGROUND: Chronic hepatitis (CH) in dogs is common and has the tendency to progress to liver cirrhosis (LC). Circulating microRNAs might have the potential as markers for disease progression.OBJECTIVES: To investigate whether concentration of specific microRNAs in serum correlate with the stage and grade of CH in Labrador Retrievers.ANIMALS: Twenty-two Labrador Retrievers with histological CH (n = 8), LC (n = 7), and normal liver (NL, n = 7).METHODS: In this retrospective study, serum concentrations of miR-122, miR-29a, miR-133a, miR-181b, and miR-17-5p were measured by quantitative real-time PCR and evaluated using univariate linear regression in dogs. A multivariate model was fit including the grade of hepatitis and the stage of fibrosis.RESULTS: Of the 5 microRNAs, only circulating miR-122 and miR-29a were significantly associated with the grade of hepatitis and the stage of fibrosis. A positive correlation was identified between the grade of hepatitis with miR-122 (rs = 0.79, P < .001) and miR-29a (rs = 0.78, P < .001). Both miR-122 (rs = 0.81, P < .001) and miR-29a (rs = 0.67, P < .001) showed a significant positive correlation with the stage of fibrosis. MiR-122 concentrations were significantly higher in the CH (P < .01) and LC groups (P < .001) compared to the NL group. MiR-29a concentrations were significantly higher in the CH (P < .001) and LC (P < .001) groups compared to the NL group.CONCLUSIONS AND CLINICAL IMPORTANCE: Circulating miR-122 and miR-29a concentrations might be useful for monitoring the response to treatment and progression of canine CH.

AB - BACKGROUND: Chronic hepatitis (CH) in dogs is common and has the tendency to progress to liver cirrhosis (LC). Circulating microRNAs might have the potential as markers for disease progression.OBJECTIVES: To investigate whether concentration of specific microRNAs in serum correlate with the stage and grade of CH in Labrador Retrievers.ANIMALS: Twenty-two Labrador Retrievers with histological CH (n = 8), LC (n = 7), and normal liver (NL, n = 7).METHODS: In this retrospective study, serum concentrations of miR-122, miR-29a, miR-133a, miR-181b, and miR-17-5p were measured by quantitative real-time PCR and evaluated using univariate linear regression in dogs. A multivariate model was fit including the grade of hepatitis and the stage of fibrosis.RESULTS: Of the 5 microRNAs, only circulating miR-122 and miR-29a were significantly associated with the grade of hepatitis and the stage of fibrosis. A positive correlation was identified between the grade of hepatitis with miR-122 (rs = 0.79, P < .001) and miR-29a (rs = 0.78, P < .001). Both miR-122 (rs = 0.81, P < .001) and miR-29a (rs = 0.67, P < .001) showed a significant positive correlation with the stage of fibrosis. MiR-122 concentrations were significantly higher in the CH (P < .01) and LC groups (P < .001) compared to the NL group. MiR-29a concentrations were significantly higher in the CH (P < .001) and LC (P < .001) groups compared to the NL group.CONCLUSIONS AND CLINICAL IMPORTANCE: Circulating miR-122 and miR-29a concentrations might be useful for monitoring the response to treatment and progression of canine CH.

KW - canine

KW - cirrhosis

KW - dog

KW - fibrosis

KW - liver injury

U2 - 10.1111/jvim.15366

DO - 10.1111/jvim.15366

M3 - Article

C2 - 30548329

SN - 0891-6640

VL - 33

SP - 151

EP - 157

JO - Journal of Veterinary Internal Medicine

JF - Journal of Veterinary Internal Medicine

IS - 1

ER -

Association of circulating microRNA-122 and microRNA-29a with stage of fibrosis and progression of chronic hepatitis in Labrador Retrievers

Abstract

Keywords

UN SDGs

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