Assessing the involvement of selected phenotypes of pseudomonas simiae PICF7 in olive root colonization and biological control of verticillium dahliae

Nuria Montes-Osuna; Carmen Gómez Lama Cabanás; Antonio Valverde-Corredor; Roeland L. Berendsen; Pilar Prieto; Jesús Mercado-Blanco

doi:10.3390/plants10020412

Assessing the involvement of selected phenotypes of pseudomonas simiae PICF7 in olive root colonization and biological control of verticillium dahliae

Nuria Montes-Osuna, Carmen Gómez Lama Cabanás, Antonio Valverde-Corredor, Roeland L. Berendsen, Pilar Prieto, Jesús Mercado-Blanco^*

^*Corresponding author for this work

CSIC - Institute for Sustainable Agriculture

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Pseudomonas simiae PICF7 is an indigenous inhabitant of the olive ( Olea europaea L.) rhizosphere/root endosphere and an effective biocontrol agent against Verticillium wilt of olive (VWO), caused by the soil-borne fungus Verticillium dahliae. This study aimed to evaluate the potential involvement of selected phenotypes of strain PICF7 in root colonization ability and VWO biocontrol. Therefore, a random transposon-insertion mutant bank of P. simiae PICF7 was screened for the loss of phenotypes likely involved in rhizosphere/soil persistence (copper resistance), root colonization (biofilm formation) and plant growth promotion (phytase activity). Transposon insertions in genes putatively coding for the transcriptional regulator CusR or the chemotaxis protein CheV were found to affect copper resistance, whereas an insertion in fleQ gene putatively encoding a flagellar regulatory protein hampered the ability to form a biofilm. However, these mutants displayed the same antagonistic effect against V. dahliae as the parental strain. Remarkably, two mutants impaired in biofilm formation were never found inside olive roots, whereas their ability to colonize the root exterior and to control VWO remained unaffected. Endophytic colonization of olive roots was unaltered in mutants impaired in copper resistance and phytase production. Results demonstrated that the phenotypes studied were irrelevant for VWO biocontrol.

Original language	English
Article number	412
Pages (from-to)	1-23
Journal	Plants
Volume	10
Issue number	2
DOIs	https://doi.org/10.3390/plants10020412
Publication status	Published - 23 Feb 2021

Bibliographical note

Funding Information:
Funding: This research was funded by the Spanish Ministerio de Economía, Industria y Competi-tividad/Agencia Estatal de Investigación, grant number AGL2016-75729-C2-1-R, and Junta de An-dalucía (Consejería de Economía, Innovación y Ciencia), grant number P12-AGR-0667, both co-financed by the European Regional Development Fund (ERDF).

Funding Information:
This research was funded by the Spanish Ministerio de Econom?a, Industria y Competitividad/Agencia Estatal de Investigaci?n, grant number AGL2016-75729-C2-1-R, and Junta de Andaluc?a (Consejer?a de Econom?a, Innovaci?n y Ciencia), grant number P12-AGR-0667, both co-financed by the European Regional Development Fund (ERDF). We thank to Mar?a Jos? Soto Misffut (Estaci?n Experimental del Zaid?n, CSIC) for her support and advice to perform the biofilm production assays, and for the gift of strain Pseudomonas putida KT2440 used in these experiments.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Funding

Funding: This research was funded by the Spanish Ministerio de Economía, Industria y Competi-tividad/Agencia Estatal de Investigación, grant number AGL2016-75729-C2-1-R, and Junta de An-dalucía (Consejería de Economía, Innovación y Ciencia), grant number P12-AGR-0667, both co-financed by the European Regional Development Fund (ERDF). This research was funded by the Spanish Ministerio de Econom?a, Industria y Competitividad/Agencia Estatal de Investigaci?n, grant number AGL2016-75729-C2-1-R, and Junta de Andaluc?a (Consejer?a de Econom?a, Innovaci?n y Ciencia), grant number P12-AGR-0667, both co-financed by the European Regional Development Fund (ERDF). We thank to Mar?a Jos? Soto Misffut (Estaci?n Experimental del Zaid?n, CSIC) for her support and advice to perform the biofilm production assays, and for the gift of strain Pseudomonas putida KT2440 used in these experiments.

Keywords

Biocontrol
Biofilm
Confocal laser scanning microscopy (CLSM)
Copper tolerance
Defoliating (D) pathotype
Endophyte
Integrated disease management
Olea europaea L
Phytase activity
Verticillium wilt of olive

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10.3390/plants10020412Licence: CC BY

plants-10-00412-v3Final published version, 2.91 MBLicence: CC BY

Cite this

@article{2222bb33f8ae40c19e9a12911959380a,

title = "Assessing the involvement of selected phenotypes of pseudomonas simiae PICF7 in olive root colonization and biological control of verticillium dahliae",

abstract = " Pseudomonas simiae PICF7 is an indigenous inhabitant of the olive ( Olea europaea L.) rhizosphere/root endosphere and an effective biocontrol agent against Verticillium wilt of olive (VWO), caused by the soil-borne fungus Verticillium dahliae. This study aimed to evaluate the potential involvement of selected phenotypes of strain PICF7 in root colonization ability and VWO biocontrol. Therefore, a random transposon-insertion mutant bank of P. simiae PICF7 was screened for the loss of phenotypes likely involved in rhizosphere/soil persistence (copper resistance), root colonization (biofilm formation) and plant growth promotion (phytase activity). Transposon insertions in genes putatively coding for the transcriptional regulator CusR or the chemotaxis protein CheV were found to affect copper resistance, whereas an insertion in fleQ gene putatively encoding a flagellar regulatory protein hampered the ability to form a biofilm. However, these mutants displayed the same antagonistic effect against V. dahliae as the parental strain. Remarkably, two mutants impaired in biofilm formation were never found inside olive roots, whereas their ability to colonize the root exterior and to control VWO remained unaffected. Endophytic colonization of olive roots was unaltered in mutants impaired in copper resistance and phytase production. Results demonstrated that the phenotypes studied were irrelevant for VWO biocontrol. ",

keywords = "Biocontrol, Biofilm, Confocal laser scanning microscopy (CLSM), Copper tolerance, Defoliating (D) pathotype, Endophyte, Integrated disease management, Olea europaea L, Phytase activity, Verticillium wilt of olive",

author = "Nuria Montes-Osuna and Caban{\'a}s, {Carmen G{\'o}mez Lama} and Antonio Valverde-Corredor and Berendsen, {Roeland L.} and Pilar Prieto and Jes{\'u}s Mercado-Blanco",

note = "Funding Information: Funding: This research was funded by the Spanish Ministerio de Econom{\'i}a, Industria y Competi-tividad/Agencia Estatal de Investigaci{\'o}n, grant number AGL2016-75729-C2-1-R, and Junta de An-daluc{\'i}a (Consejer{\'i}a de Econom{\'i}a, Innovaci{\'o}n y Ciencia), grant number P12-AGR-0667, both co-financed by the European Regional Development Fund (ERDF). Funding Information: This research was funded by the Spanish Ministerio de Econom?a, Industria y Competitividad/Agencia Estatal de Investigaci?n, grant number AGL2016-75729-C2-1-R, and Junta de Andaluc?a (Consejer?a de Econom?a, Innovaci?n y Ciencia), grant number P12-AGR-0667, both co-financed by the European Regional Development Fund (ERDF). We thank to Mar?a Jos? Soto Misffut (Estaci?n Experimental del Zaid?n, CSIC) for her support and advice to perform the biofilm production assays, and for the gift of strain Pseudomonas putida KT2440 used in these experiments. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = feb,

day = "23",

doi = "10.3390/plants10020412",

language = "English",

volume = "10",

pages = "1--23",

journal = "Plants",

issn = "2223-7747",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "2",

}

Assessing the involvement of selected phenotypes of pseudomonas simiae PICF7 in olive root colonization and biological control of verticillium dahliae. / Montes-Osuna, Nuria; Cabanás, Carmen Gómez Lama; Valverde-Corredor, Antonio et al.
In: Plants, Vol. 10, No. 2, 412, 23.02.2021, p. 1-23.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Assessing the involvement of selected phenotypes of pseudomonas simiae PICF7 in olive root colonization and biological control of verticillium dahliae

AU - Montes-Osuna, Nuria

AU - Cabanás, Carmen Gómez Lama

AU - Valverde-Corredor, Antonio

AU - Berendsen, Roeland L.

AU - Prieto, Pilar

AU - Mercado-Blanco, Jesús

N1 - Funding Information: Funding: This research was funded by the Spanish Ministerio de Economía, Industria y Competi-tividad/Agencia Estatal de Investigación, grant number AGL2016-75729-C2-1-R, and Junta de An-dalucía (Consejería de Economía, Innovación y Ciencia), grant number P12-AGR-0667, both co-financed by the European Regional Development Fund (ERDF). Funding Information: This research was funded by the Spanish Ministerio de Econom?a, Industria y Competitividad/Agencia Estatal de Investigaci?n, grant number AGL2016-75729-C2-1-R, and Junta de Andaluc?a (Consejer?a de Econom?a, Innovaci?n y Ciencia), grant number P12-AGR-0667, both co-financed by the European Regional Development Fund (ERDF). We thank to Mar?a Jos? Soto Misffut (Estaci?n Experimental del Zaid?n, CSIC) for her support and advice to perform the biofilm production assays, and for the gift of strain Pseudomonas putida KT2440 used in these experiments. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/2/23

Y1 - 2021/2/23

N2 - Pseudomonas simiae PICF7 is an indigenous inhabitant of the olive ( Olea europaea L.) rhizosphere/root endosphere and an effective biocontrol agent against Verticillium wilt of olive (VWO), caused by the soil-borne fungus Verticillium dahliae. This study aimed to evaluate the potential involvement of selected phenotypes of strain PICF7 in root colonization ability and VWO biocontrol. Therefore, a random transposon-insertion mutant bank of P. simiae PICF7 was screened for the loss of phenotypes likely involved in rhizosphere/soil persistence (copper resistance), root colonization (biofilm formation) and plant growth promotion (phytase activity). Transposon insertions in genes putatively coding for the transcriptional regulator CusR or the chemotaxis protein CheV were found to affect copper resistance, whereas an insertion in fleQ gene putatively encoding a flagellar regulatory protein hampered the ability to form a biofilm. However, these mutants displayed the same antagonistic effect against V. dahliae as the parental strain. Remarkably, two mutants impaired in biofilm formation were never found inside olive roots, whereas their ability to colonize the root exterior and to control VWO remained unaffected. Endophytic colonization of olive roots was unaltered in mutants impaired in copper resistance and phytase production. Results demonstrated that the phenotypes studied were irrelevant for VWO biocontrol.

AB - Pseudomonas simiae PICF7 is an indigenous inhabitant of the olive ( Olea europaea L.) rhizosphere/root endosphere and an effective biocontrol agent against Verticillium wilt of olive (VWO), caused by the soil-borne fungus Verticillium dahliae. This study aimed to evaluate the potential involvement of selected phenotypes of strain PICF7 in root colonization ability and VWO biocontrol. Therefore, a random transposon-insertion mutant bank of P. simiae PICF7 was screened for the loss of phenotypes likely involved in rhizosphere/soil persistence (copper resistance), root colonization (biofilm formation) and plant growth promotion (phytase activity). Transposon insertions in genes putatively coding for the transcriptional regulator CusR or the chemotaxis protein CheV were found to affect copper resistance, whereas an insertion in fleQ gene putatively encoding a flagellar regulatory protein hampered the ability to form a biofilm. However, these mutants displayed the same antagonistic effect against V. dahliae as the parental strain. Remarkably, two mutants impaired in biofilm formation were never found inside olive roots, whereas their ability to colonize the root exterior and to control VWO remained unaffected. Endophytic colonization of olive roots was unaltered in mutants impaired in copper resistance and phytase production. Results demonstrated that the phenotypes studied were irrelevant for VWO biocontrol.

KW - Biocontrol

KW - Biofilm

KW - Confocal laser scanning microscopy (CLSM)

KW - Copper tolerance

KW - Defoliating (D) pathotype

KW - Endophyte

KW - Integrated disease management

KW - Olea europaea L

KW - Phytase activity

KW - Verticillium wilt of olive

U2 - 10.3390/plants10020412

DO - 10.3390/plants10020412

M3 - Article

C2 - 33672351

AN - SCOPUS:85101203537

SN - 2223-7747

VL - 10

SP - 1

EP - 23

JO - Plants

JF - Plants

IS - 2

M1 - 412

ER -

Assessing the involvement of selected phenotypes of pseudomonas simiae PICF7 in olive root colonization and biological control of verticillium dahliae

Abstract

Bibliographical note

Funding

Keywords

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