Assembly of HCV E1 and E2 glycoproteins into coronavirus VLPs

N. Jourdan; G.J. Godeke; M. Penaud; G. Mottola; A. Sorrentino; P.J.M. Rottier; S Bonatti

doi:10.1007/s00705-006-0769-0

Assembly of HCV E1 and E2 glycoproteins into coronavirus VLPs

N. Jourdan
, G.J. Godeke
, M. Penaud
, G. Mottola
, A. Sorrentino
, P.J.M. Rottier
, S Bonatti

extern

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Hepatitis C virus (HCV) is believed to assemble by budding into membranes of the early secretory pathway, consistent with the membrane location where the viral envelope glycoproteins E1 and E2 accumulate when expressed. Coronavirus assembly also takes place at pre-Golgi membranes. Here, we generated coronavirus-like particles carrying in their envelope chimeric HCV glycoproteins composed of the ectodomains of E1 and E2, each fused to the transmembrane plus endodomain of the mouse hepatitis coronavirus spike glycoprotein. The chimeric particle system will enable structural and functional studies of the HCV glycoproteins.

Original language	English
Pages (from-to)	2085-2094
Number of pages	10
Journal	Archives of Virology
Volume	151
Issue number	10
DOIs	https://doi.org/10.1007/s00705-006-0769-0
Publication status	Published - 2006

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SDG 3 Good Health and Well-being

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abstract = "Hepatitis C virus (HCV) is believed to assemble by budding into membranes of the early secretory pathway, consistent with the membrane location where the viral envelope glycoproteins E1 and E2 accumulate when expressed. Coronavirus assembly also takes place at pre-Golgi membranes. Here, we generated coronavirus-like particles carrying in their envelope chimeric HCV glycoproteins composed of the ectodomains of E1 and E2, each fused to the transmembrane plus endodomain of the mouse hepatitis coronavirus spike glycoprotein. The chimeric particle system will enable structural and functional studies of the HCV glycoproteins.",

author = "N. Jourdan and G.J. Godeke and M. Penaud and G. Mottola and A. Sorrentino and P.J.M. Rottier and S Bonatti",

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AU - Jourdan, N.

AU - Godeke, G.J.

AU - Penaud, M.

AU - Mottola, G.

AU - Sorrentino, A.

AU - Rottier, P.J.M.

AU - Bonatti, S

PY - 2006

Y1 - 2006

N2 - Hepatitis C virus (HCV) is believed to assemble by budding into membranes of the early secretory pathway, consistent with the membrane location where the viral envelope glycoproteins E1 and E2 accumulate when expressed. Coronavirus assembly also takes place at pre-Golgi membranes. Here, we generated coronavirus-like particles carrying in their envelope chimeric HCV glycoproteins composed of the ectodomains of E1 and E2, each fused to the transmembrane plus endodomain of the mouse hepatitis coronavirus spike glycoprotein. The chimeric particle system will enable structural and functional studies of the HCV glycoproteins.

AB - Hepatitis C virus (HCV) is believed to assemble by budding into membranes of the early secretory pathway, consistent with the membrane location where the viral envelope glycoproteins E1 and E2 accumulate when expressed. Coronavirus assembly also takes place at pre-Golgi membranes. Here, we generated coronavirus-like particles carrying in their envelope chimeric HCV glycoproteins composed of the ectodomains of E1 and E2, each fused to the transmembrane plus endodomain of the mouse hepatitis coronavirus spike glycoprotein. The chimeric particle system will enable structural and functional studies of the HCV glycoproteins.

U2 - 10.1007/s00705-006-0769-0

DO - 10.1007/s00705-006-0769-0

M3 - Article

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JO - Archives of Virology

JF - Archives of Virology

IS - 10

ER -

Assembly of HCV E1 and E2 glycoproteins into coronavirus VLPs

Abstract

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