Arabidopsis Topless-related 1 mitigates physiological damage and growth penalties of induced immunity

Thomas Griebel, Dmitry Lapin, Federica Locci, Barbara Kracher, Jaqueline Bautor, Lorenzo Concia, Moussa Benhamed, Jane E Parker

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Transcriptional corepressors of the Topless (TPL) family regulate plant hormone and immunity signaling. The lack of a genome-wide profile of their chromatin associations limits understanding of the TPL family roles in transcriptional regulation. Chromatin immunoprecipitation with sequencing (ChIP-Seq) was performed on Arabidopsis thaliana lines expressing GFP-tagged Topless-related 1 (TPR1-GFP) with and without constitutive immunity via Enhanced Disease Susceptibility 1 (EDS1). RNA-Seq profiling of the TPR1-GFP lines and pathogen-infected tpl/tpr mutants, combined with measuring immunity, growth, and physiological parameters was employed to investigate TPL/TPR roles in immunity and defense homeostasis. TPR1 was enriched at promoter regions of c. 1400 genes and c. 10% of the detected binding required EDS1 immunity signaling. In a tpr1 tpl tpr4 (t3) mutant, resistance to bacteria was slightly compromised, and defense-related transcriptional reprogramming was weakly reduced or enhanced, respectively, at early (< 1 h) and late 24 h stages of bacterial infection. The t3 plants challenged with bacteria or pathogen-associated molecular pattern nlp24 displayed photosystem II dysfunctions. Also, t3 plants were hypersensitive to phytocytokine pep1 at the level of root growth inhibition. Transgenic expression of TPR1 rescued these t3 physiological defects. We propose that TPR1 and TPL family proteins function in Arabidopsis to reduce detrimental effects associated with activated transcriptional immunity.

Original languageEnglish
Pages (from-to)1404-1419
Number of pages16
JournalNew Phytologist
Volume239
Issue number4
Early online date12 Jun 2023
DOIs
Publication statusPublished - Aug 2023

Keywords

  • ChIP-Seq
  • Topless-related 1
  • corepressor
  • immunity
  • resilience

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