Application of minimal sequence quality values prevents misidentification of the blaSHV type in single bacterial isolates carrying different SHV extended-spectrum beta-lactamase genes

Nashwan Al Naiemi; Kim Schipper; Birgitta Duim; Aldert Bart

doi:10.1128/JCM.44.5.1896-1898.2006

Application of minimal sequence quality values prevents misidentification of the blaSHV type in single bacterial isolates carrying different SHV extended-spectrum beta-lactamase genes

Nashwan Al Naiemi, Kim Schipper, Birgitta Duim, Aldert Bart

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Nucleotide sequencing is the standard molecular method for determination of the beta-lactamase gene present in an isolate. Using minimal sequence quality values prevents misidentification of bla(SHV) genes, as illustrated by three strains of three different species that each contained two different bla(SHV) alleles, SHV-2 and SHV-12.

Original language	English
Pages (from-to)	1896-8
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	44
Issue number	5
DOIs	https://doi.org/10.1128/JCM.44.5.1896-1898.2006
Publication status	Published - 2006

Keywords

Alleles
Bacteria
Base Sequence
DNA, Bacterial
Enterobacter cloacae
Escherichia coli
Genes, Bacterial
Humans
Polymerase Chain Reaction
Pseudomonas aeruginosa
beta-Lactamases

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10.1128/JCM.44.5.1896-1898.2006

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title = "Application of minimal sequence quality values prevents misidentification of the blaSHV type in single bacterial isolates carrying different SHV extended-spectrum beta-lactamase genes",

abstract = "Nucleotide sequencing is the standard molecular method for determination of the beta-lactamase gene present in an isolate. Using minimal sequence quality values prevents misidentification of bla(SHV) genes, as illustrated by three strains of three different species that each contained two different bla(SHV) alleles, SHV-2 and SHV-12.",

keywords = "Alleles, Bacteria, Base Sequence, DNA, Bacterial, Enterobacter cloacae, Escherichia coli, Genes, Bacterial, Humans, Polymerase Chain Reaction, Pseudomonas aeruginosa, beta-Lactamases",

author = "{Al Naiemi}, Nashwan and Kim Schipper and Birgitta Duim and Aldert Bart",

year = "2006",

doi = "10.1128/JCM.44.5.1896-1898.2006",

language = "English",

volume = "44",

pages = "1896--8",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "5",

}

Application of minimal sequence quality values prevents misidentification of the blaSHV type in single bacterial isolates carrying different SHV extended-spectrum beta-lactamase genes. / Al Naiemi, Nashwan; Schipper, Kim; Duim, Birgitta et al.
In: Journal of Clinical Microbiology, Vol. 44, No. 5, 2006, p. 1896-8.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Application of minimal sequence quality values prevents misidentification of the blaSHV type in single bacterial isolates carrying different SHV extended-spectrum beta-lactamase genes

AU - Al Naiemi, Nashwan

AU - Schipper, Kim

AU - Duim, Birgitta

AU - Bart, Aldert

PY - 2006

Y1 - 2006

N2 - Nucleotide sequencing is the standard molecular method for determination of the beta-lactamase gene present in an isolate. Using minimal sequence quality values prevents misidentification of bla(SHV) genes, as illustrated by three strains of three different species that each contained two different bla(SHV) alleles, SHV-2 and SHV-12.

AB - Nucleotide sequencing is the standard molecular method for determination of the beta-lactamase gene present in an isolate. Using minimal sequence quality values prevents misidentification of bla(SHV) genes, as illustrated by three strains of three different species that each contained two different bla(SHV) alleles, SHV-2 and SHV-12.

KW - Alleles

KW - Bacteria

KW - Base Sequence

KW - DNA, Bacterial

KW - Enterobacter cloacae

KW - Escherichia coli

KW - Genes, Bacterial

KW - Humans

KW - Polymerase Chain Reaction

KW - Pseudomonas aeruginosa

KW - beta-Lactamases

U2 - 10.1128/JCM.44.5.1896-1898.2006

DO - 10.1128/JCM.44.5.1896-1898.2006

M3 - Article

C2 - 16672434

SN - 0095-1137

VL - 44

SP - 1896

EP - 1898

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 5

ER -

Application of minimal sequence quality values prevents misidentification of the blaSHV type in single bacterial isolates carrying different SHV extended-spectrum beta-lactamase genes

Abstract

Keywords

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