Antibodies against MERS coronavirus in dromedaries, United Arab Emirates, 2003 and 2013

Benjamin Meyer; Marcel A. Müller; Victor M. Corman; Chantal B E M Reusken; Daniel Ritz; Gert Jan Godeke; Erik Lattwein; Stephan Kallies; Artem Siemens; Janko van Beek; Jan F. Drexler; Doreen Muth; Berend Jan Bosch; Ulrich Wernery; Marion P G Koopmans; Renate Wernery; Christian Drosten

doi:10.3201/eid2004.131746

Antibodies against MERS coronavirus in dromedaries, United Arab Emirates, 2003 and 2013

Benjamin Meyer, Marcel A. Müller, Victor M. Corman, Chantal B E M Reusken, Daniel Ritz, Gert Jan Godeke, Erik Lattwein, Stephan Kallies, Artem Siemens, Janko van Beek, Jan F. Drexler, Doreen Muth, Berend Jan Bosch, Ulrich Wernery, Marion P G Koopmans, Renate Wernery, Christian Drosten

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Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) has caused an ongoing outbreak of severe acute respiratory tract infection in humans in the Arabian Peninsula since 2012. Dromedary camels have been implicated as possible viral reservoirs. We used serologic assays to analyze 651 dromedary camel serum samples from the United Arab Emirates; 151 of 651 samples were obtained in 2003, well before onset of the current epidemic, and 500 serum samples were obtained in 2013. Recombinant spike protein-specific immunofluorescence and virus neutralization tests enabled clear discrimination between MERS-CoV and bovine CoV infections. Most (632/651, 97.1%) camels had antibodies against MERS-CoV. This result included all 151 serum samples obtained in 2003. Most (389/651, 59.8%) serum samples had MERS-CoV-neutralizing antibody titers >1,280. Dromedary camels from the United Arab Emirates were infected at high rates with MERS-CoV or a closely related, probably conspecific, virus long before the first human MERS cases.

Original language	English
Pages (from-to)	552-559
Number of pages	8
Journal	Emerging Infectious Diseases
Volume	20
Issue number	4
DOIs	https://doi.org/10.3201/eid2004.131746
Publication status	Published - 2014

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Meyer, B., Müller, M. A., Corman, V. M., Reusken, C. B. E. M., Ritz, D., Godeke, G. J., Lattwein, E., Kallies, S., Siemens, A., van Beek, J., Drexler, J. F., Muth, D., Bosch, B. J., Wernery, U., Koopmans, M. P. G., Wernery, R., & Drosten, C. (2014). Antibodies against MERS coronavirus in dromedaries, United Arab Emirates, 2003 and 2013. Emerging Infectious Diseases, 20(4), 552-559. https://doi.org/10.3201/eid2004.131746

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title = "Antibodies against MERS coronavirus in dromedaries, United Arab Emirates, 2003 and 2013",

abstract = "Middle East respiratory syndrome coronavirus (MERS-CoV) has caused an ongoing outbreak of severe acute respiratory tract infection in humans in the Arabian Peninsula since 2012. Dromedary camels have been implicated as possible viral reservoirs. We used serologic assays to analyze 651 dromedary camel serum samples from the United Arab Emirates; 151 of 651 samples were obtained in 2003, well before onset of the current epidemic, and 500 serum samples were obtained in 2013. Recombinant spike protein-specific immunofluorescence and virus neutralization tests enabled clear discrimination between MERS-CoV and bovine CoV infections. Most (632/651, 97.1%) camels had antibodies against MERS-CoV. This result included all 151 serum samples obtained in 2003. Most (389/651, 59.8%) serum samples had MERS-CoV-neutralizing antibody titers >1,280. Dromedary camels from the United Arab Emirates were infected at high rates with MERS-CoV or a closely related, probably conspecific, virus long before the first human MERS cases.",

author = "Benjamin Meyer and M{\"u}ller, {Marcel A.} and Corman, {Victor M.} and Reusken, {Chantal B E M} and Daniel Ritz and Godeke, {Gert Jan} and Erik Lattwein and Stephan Kallies and Artem Siemens and {van Beek}, Janko and Drexler, {Jan F.} and Doreen Muth and Bosch, {Berend Jan} and Ulrich Wernery and Koopmans, {Marion P G} and Renate Wernery and Christian Drosten",

year = "2014",

doi = "10.3201/eid2004.131746",

language = "English",

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Meyer, B, Müller, MA, Corman, VM, Reusken, CBEM, Ritz, D, Godeke, GJ, Lattwein, E, Kallies, S, Siemens, A, van Beek, J, Drexler, JF, Muth, D, Bosch, BJ, Wernery, U, Koopmans, MPG, Wernery, R & Drosten, C 2014, 'Antibodies against MERS coronavirus in dromedaries, United Arab Emirates, 2003 and 2013', Emerging Infectious Diseases, vol. 20, no. 4, pp. 552-559. https://doi.org/10.3201/eid2004.131746

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T1 - Antibodies against MERS coronavirus in dromedaries, United Arab Emirates, 2003 and 2013

AU - Meyer, Benjamin

AU - Müller, Marcel A.

AU - Corman, Victor M.

AU - Reusken, Chantal B E M

AU - Ritz, Daniel

AU - Godeke, Gert Jan

AU - Lattwein, Erik

AU - Kallies, Stephan

AU - Siemens, Artem

AU - van Beek, Janko

AU - Drexler, Jan F.

AU - Muth, Doreen

AU - Bosch, Berend Jan

AU - Wernery, Ulrich

AU - Koopmans, Marion P G

AU - Wernery, Renate

AU - Drosten, Christian

PY - 2014

Y1 - 2014

N2 - Middle East respiratory syndrome coronavirus (MERS-CoV) has caused an ongoing outbreak of severe acute respiratory tract infection in humans in the Arabian Peninsula since 2012. Dromedary camels have been implicated as possible viral reservoirs. We used serologic assays to analyze 651 dromedary camel serum samples from the United Arab Emirates; 151 of 651 samples were obtained in 2003, well before onset of the current epidemic, and 500 serum samples were obtained in 2013. Recombinant spike protein-specific immunofluorescence and virus neutralization tests enabled clear discrimination between MERS-CoV and bovine CoV infections. Most (632/651, 97.1%) camels had antibodies against MERS-CoV. This result included all 151 serum samples obtained in 2003. Most (389/651, 59.8%) serum samples had MERS-CoV-neutralizing antibody titers >1,280. Dromedary camels from the United Arab Emirates were infected at high rates with MERS-CoV or a closely related, probably conspecific, virus long before the first human MERS cases.

AB - Middle East respiratory syndrome coronavirus (MERS-CoV) has caused an ongoing outbreak of severe acute respiratory tract infection in humans in the Arabian Peninsula since 2012. Dromedary camels have been implicated as possible viral reservoirs. We used serologic assays to analyze 651 dromedary camel serum samples from the United Arab Emirates; 151 of 651 samples were obtained in 2003, well before onset of the current epidemic, and 500 serum samples were obtained in 2013. Recombinant spike protein-specific immunofluorescence and virus neutralization tests enabled clear discrimination between MERS-CoV and bovine CoV infections. Most (632/651, 97.1%) camels had antibodies against MERS-CoV. This result included all 151 serum samples obtained in 2003. Most (389/651, 59.8%) serum samples had MERS-CoV-neutralizing antibody titers >1,280. Dromedary camels from the United Arab Emirates were infected at high rates with MERS-CoV or a closely related, probably conspecific, virus long before the first human MERS cases.

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DO - 10.3201/eid2004.131746

M3 - Article

C2 - 24655412

SN - 1080-6040

VL - 20

SP - 552

EP - 559

JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

IS - 4

ER -

Antibodies against MERS coronavirus in dromedaries, United Arab Emirates, 2003 and 2013

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