Angiogenic capacity of human adipose-derived stromal cells during adipogenic differentiation: an in vitro study

Femke Verseijden, Holger Jahr, Sandra J Posthumus-van Sluijs, Timo L Ten Hagen, Steven E R Hovius, Ann L B Seynhaeve, Johan W van Neck, Gerjo J V M van Osch, Stefan O P Hofer

Research output: Contribution to journalArticleAcademicpeer-review


BACKGROUND: Improving vascularization of engineered adipose tissue constructs is a major challenge in the field of plastic surgery. Although human adipose-derived stromal cells (hASCs) are known to release factors that stimulate new blood vessel formation, detailed information about the effects of adipogenic differentiation on the angiogenic potential of hASCs remains largely unknown. In the present study, we studied the expression and secretion of a large panel of angiogenic factors during hASC differentiation and evaluated the effects of hASC-conditioned medium (hASC-CM) on endothelial cells.

METHODS: hASCs were cultured on adipogenic medium or basal medium. Conditioned medium was collected, and cells were harvested following 0, 3, 7, 14, and 22 days of culture. The stage of adipogenic differentiation of hASC was assessed using Oil Red O staining, fatty acid binding protein-4 gene expression, and glycerol-3-phosphate dehydrogenase activity.

RESULTS: Gene expression of vascular endothelial growth factor (VEGF), placental growth factor, angiopoietin-1 (ANGPT1), angiopoietin-2 (ANGPT2), and protein secretion of VEGF significantly increased during short-term adipogenic differentiation of hASCs. Moreover, conditioned medium from differentiated hASCs strongly enhanced endothelial cell numbers compared to conditioned medium from undifferentiated hASCs.

CONCLUSION: In vitro adipogenic differentiation of hASCs improves their ability to support endothelial viable cell numbers and suggests that hASCs differentiated for a short period potentially improve angiogenic responses for in vivo implantation.

Original languageEnglish
Pages (from-to)445-52
Number of pages8
JournalTissue Engineering - Part A.
Issue number2
Publication statusPublished - Feb 2009
Externally publishedYes


  • Adipocytes
  • Adipogenesis
  • Adipose Tissue
  • Adult
  • Angiogenesis Inducing Agents
  • Azo Compounds
  • Cell Count
  • Cell Lineage
  • Cell Proliferation
  • Cell Survival
  • Cells, Cultured
  • Culture Media, Conditioned
  • Endothelial Cells
  • Fatty Acid-Binding Proteins
  • Gene Expression Regulation
  • Glycerolphosphate Dehydrogenase
  • Humans
  • Middle Aged
  • Neovascularization, Physiologic
  • Staining and Labeling
  • Stromal Cells
  • Vascular Endothelial Growth Factor A
  • Journal Article
  • Research Support, Non-U.S. Gov't


Dive into the research topics of 'Angiogenic capacity of human adipose-derived stromal cells during adipogenic differentiation: an in vitro study'. Together they form a unique fingerprint.

Cite this