Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365

Dujuan Liu; Sandra Garrigues; Helena Culleton; Vincent A. McKie; Ronald P. de Vries

doi:10.1016/j.nbt.2024.04.003

Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365

Dujuan Liu, Sandra Garrigues, Helena Culleton, Vincent A. McKie, Ronald P. de Vries^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Aspergillus vadensis CBS 113365, a close relative of A. niger, has been suggested as a more favourable alternative for recombinant protein production as it does not acidify the culture medium and produces very low levels of extracellular proteases. The aim of this study was to investigate the underlying cause of the non-amylolytic and non-proteolytic phenotype of A. vadensis CBS 113365. Our results demonstrate that the non-functionality of the amylolytic transcription factor AmyR in A. vadensis CBS 113365 is primarily attributed to the lack of functionality of its gene's promoter sequence. In contrast, a different mechanism is likely causing the lack of PrtT activity, which is the main transcriptional regulator of protease production. The findings presented here not only expand our understanding of the genetic basis behind the distinct characteristics of A. vadensis CBS 113365, but also underscore its potential as a favourable alternative for recombinant protein production.

Original language	English
Pages (from-to)	25-32
Number of pages	8
Journal	New Biotechnology
Volume	82
DOIs	https://doi.org/10.1016/j.nbt.2024.04.003
Publication status	Published - 25 Sept 2024

Bibliographical note

Publisher Copyright:
© 2024 The Authors

Funding

This research was supported by the China Scholarship Council for financial support (grant no. 202107720032) . SG. was supported by a grant of the Applied Science Division (TTW) of NWO and the Biotechnology and Safety Program of the Ministry of Infrastructure and Water Management 15807 to RPdV. SG currently holds a Juan de la Cierva Incorporacion grant (IJC 2020-042749-I) funded by MCIN/AEI/10.13039/501100011033 and the European Union Next Generation EU/PRTR.

Funders	Funder number
China Scholarship Council	202107720032
Applied Science Division (TTW) of NWO
Biotechnology and Safety Program of the Ministry of Infrastructure and Water Management	15807
Juan de la Cierva Incorporacion -MCIN/AEI/	IJC 2020-042749-I
European Union Next Generation EU/PRTR

Keywords

AmyR
Aspergillus vadensis
Gene regulation
Genetic swaps
PrtT
Transcriptional activator

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1-s2.0-S1871678424000141-mainFinal published version, 3.61 MBLicence: CC BY

Cite this

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title = "Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365",

abstract = "Aspergillus vadensis CBS 113365, a close relative of A. niger, has been suggested as a more favourable alternative for recombinant protein production as it does not acidify the culture medium and produces very low levels of extracellular proteases. The aim of this study was to investigate the underlying cause of the non-amylolytic and non-proteolytic phenotype of A. vadensis CBS 113365. Our results demonstrate that the non-functionality of the amylolytic transcription factor AmyR in A. vadensis CBS 113365 is primarily attributed to the lack of functionality of its gene's promoter sequence. In contrast, a different mechanism is likely causing the lack of PrtT activity, which is the main transcriptional regulator of protease production. The findings presented here not only expand our understanding of the genetic basis behind the distinct characteristics of A. vadensis CBS 113365, but also underscore its potential as a favourable alternative for recombinant protein production.",

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author = "Dujuan Liu and Sandra Garrigues and Helena Culleton and McKie, {Vincent A.} and {de Vries}, {Ronald P.}",

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AU - Culleton, Helena

AU - McKie, Vincent A.

AU - de Vries, Ronald P.

PY - 2024/9/25

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Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365

Abstract

Bibliographical note

Funding

Keywords

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