Analysis of ORF5 sequences of Porcine Reproductive and Respiratory Syndrome virus (PRRSV) circulating within swine farms in Costa Rica

Mónica Guzmán; Ronald Meléndez; Carlos Jiménez; Marta Piche; Emily Jiménez; Bernal León; Juan M Cordero; Lisbeth Ramirez-Carvajal; Alberto Uribe; Arie Van Nes; Arjan Stegeman; Juan José Romero

doi:10.1186/s12917-021-02925-7

Analysis of ORF5 sequences of Porcine Reproductive and Respiratory Syndrome virus (PRRSV) circulating within swine farms in Costa Rica

Mónica Guzmán
, Ronald Meléndez
, Carlos Jiménez
, Marta Piche
, Emily Jiménez
, Bernal León
, Juan M Cordero
, Lisbeth Ramirez-Carvajal
, Alberto Uribe
, Arie Van Nes
, Arjan Stegeman
, Juan José Romero

Department of Veterinary Diagnostics (DDV)
Utrecht University, University Medical Center Utrecht
School of Veterinary Medicine (EMV)
Faryvet Company
Boehringer Ingelheim GmbH

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: Worldwide, Porcine Reproductive and Respiratory Syndrome (PRRS) is among the diseases that cause the highest economic impact in modern pig production. PRRS was first detected in Costa Rica in 1996 and has since then severely affected the local swine industry. Studies of the molecular characterization of circulating strains, correlation with clinical records, and associations with pathogens associated with Porcine Respiratory Disease Complex (PRDC) have not been done in Costa Rica.

RESULTS: Sequencing and phylogenetic analysis of ORF5 proved that PRRSV-2 was the only species detected in all locations analyzed. These sequences were grouped into three clusters. When comparing samples from San Jose, Alejuela, and Puntarenas to historical isolates of the previously described lineages (1 to 9), it has been shown that these were closely related to each other and belonged to Lineage 5, along with the samples from Heredia. Intriguingly, samples from Cartago clustered in a separate clade, phylogenetically related to Lineage 1. Epitope analysis conducted on the GP5 sequence of field isolates from Costa Rica revealed seven peptides with at least 80% amino acid sequence identity with previously described and experimentally validated immunogenic regions. Previously described epitopes A, B, and C, were detected in the Santa Barbara-Heredia isolate.

CONCLUSIONS: Our data suggest that the virus has three distinct origins or introductions to the country. Future studies will elucidate how recently introduced vaccines will shape the evolutionary change of circulating field strains.

Original language	English
Article number	217
Pages (from-to)	1-11
Journal	BMC Veterinary Research
Volume	17
Issue number	1
DOIs	https://doi.org/10.1186/s12917-021-02925-7
Publication status	Published - Dec 2021

Bibliographical note

Funding Information:
Was a recipient of a from the Ministry of Science and Technology and Telecommunications (MICITT) of Costa Rica. This work was funded by PND-018-15-2 from PINN POSGRADOS Ministerio de Ciencia, Tecnología y Telecomunicaciones (MICITT), a Doctoral scholarship for RM. Financial contribution to this study also came from 0174-10 CRIPAS, UNA Costa Rica and the Department of Population Health Sciences of Utrecht University.

Funding Information:
The authors acknowledge the contribution of the technical staff from the Virology laboratory EMV, UNA, and the Biosecurity Laboratory, LANASEVE, SENASA. Also, thanks go to Susana Ure?a for helping in sample collection and epidemiology data from farms used in this study. The authors thank Prof Hans Nauwynck and Boehringer Ingelheim for providing laboratory supplies and technical assistance and Sietske Ruijgh for the editing.

Publisher Copyright:
© 2021, The Author(s).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Costa Rica
Glycoprotein 5(GP5)
Open reading frame 5 (ORF5)
Porcine Reproductive and Respiratory Syndrome (PRRS)
Porcine Respiratory Disease Complex (PRDC)
phylogenetic tree

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Guzmán, M., Meléndez, R., Jiménez, C., Piche, M., Jiménez, E., León, B., Cordero, J. M., Ramirez-Carvajal, L., Uribe, A., Van Nes, A., Stegeman, A., & Romero, J. J. (2021). Analysis of ORF5 sequences of Porcine Reproductive and Respiratory Syndrome virus (PRRSV) circulating within swine farms in Costa Rica. BMC Veterinary Research, 17(1), 1-11. Article 217. https://doi.org/10.1186/s12917-021-02925-7

@article{47b5bfb36fd6431e80b173d26a00786d,

title = "Analysis of ORF5 sequences of Porcine Reproductive and Respiratory Syndrome virus (PRRSV) circulating within swine farms in Costa Rica",

abstract = "BACKGROUND: Worldwide, Porcine Reproductive and Respiratory Syndrome (PRRS) is among the diseases that cause the highest economic impact in modern pig production. PRRS was first detected in Costa Rica in 1996 and has since then severely affected the local swine industry. Studies of the molecular characterization of circulating strains, correlation with clinical records, and associations with pathogens associated with Porcine Respiratory Disease Complex (PRDC) have not been done in Costa Rica.RESULTS: Sequencing and phylogenetic analysis of ORF5 proved that PRRSV-2 was the only species detected in all locations analyzed. These sequences were grouped into three clusters. When comparing samples from San Jose, Alejuela, and Puntarenas to historical isolates of the previously described lineages (1 to 9), it has been shown that these were closely related to each other and belonged to Lineage 5, along with the samples from Heredia. Intriguingly, samples from Cartago clustered in a separate clade, phylogenetically related to Lineage 1. Epitope analysis conducted on the GP5 sequence of field isolates from Costa Rica revealed seven peptides with at least 80\% amino acid sequence identity with previously described and experimentally validated immunogenic regions. Previously described epitopes A, B, and C, were detected in the Santa Barbara-Heredia isolate.CONCLUSIONS: Our data suggest that the virus has three distinct origins or introductions to the country. Future studies will elucidate how recently introduced vaccines will shape the evolutionary change of circulating field strains.",

keywords = "Costa Rica, Glycoprotein 5(GP5), Open reading frame 5 (ORF5), Porcine Reproductive and Respiratory Syndrome (PRRS), Porcine Respiratory Disease Complex (PRDC), phylogenetic tree",

author = "M{\'o}nica Guzm{\'a}n and Ronald Mel{\'e}ndez and Carlos Jim{\'e}nez and Marta Piche and Emily Jim{\'e}nez and Bernal Le{\'o}n and Cordero, \{Juan M\} and Lisbeth Ramirez-Carvajal and Alberto Uribe and \{Van Nes\}, Arie and Arjan Stegeman and Romero, \{Juan Jos{\'e}\}",

note = "Funding Information: Was a recipient of a from the Ministry of Science and Technology and Telecommunications (MICITT) of Costa Rica. This work was funded by PND-018-15-2 from PINN POSGRADOS Ministerio de Ciencia, Tecnolog{\'i}a y Telecomunicaciones (MICITT), a Doctoral scholarship for RM. Financial contribution to this study also came from 0174-10 CRIPAS, UNA Costa Rica and the Department of Population Health Sciences of Utrecht University. Funding Information: The authors acknowledge the contribution of the technical staff from the Virology laboratory EMV, UNA, and the Biosecurity Laboratory, LANASEVE, SENASA. Also, thanks go to Susana Ure?a for helping in sample collection and epidemiology data from farms used in this study. The authors thank Prof Hans Nauwynck and Boehringer Ingelheim for providing laboratory supplies and technical assistance and Sietske Ruijgh for the editing. Publisher Copyright: {\textcopyright} 2021, The Author(s).",

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month = dec,

doi = "10.1186/s12917-021-02925-7",

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Guzmán, M, Meléndez, R, Jiménez, C, Piche, M, Jiménez, E, León, B, Cordero, JM, Ramirez-Carvajal, L, Uribe, A, Van Nes, A , Stegeman, A & Romero, JJ 2021, 'Analysis of ORF5 sequences of Porcine Reproductive and Respiratory Syndrome virus (PRRSV) circulating within swine farms in Costa Rica', BMC Veterinary Research, vol. 17, no. 1, 217, pp. 1-11. https://doi.org/10.1186/s12917-021-02925-7

TY - JOUR

T1 - Analysis of ORF5 sequences of Porcine Reproductive and Respiratory Syndrome virus (PRRSV) circulating within swine farms in Costa Rica

AU - Guzmán, Mónica

AU - Meléndez, Ronald

AU - Jiménez, Carlos

AU - Piche, Marta

AU - Jiménez, Emily

AU - León, Bernal

AU - Cordero, Juan M

AU - Ramirez-Carvajal, Lisbeth

AU - Uribe, Alberto

AU - Van Nes, Arie

AU - Stegeman, Arjan

AU - Romero, Juan José

N1 - Funding Information: Was a recipient of a from the Ministry of Science and Technology and Telecommunications (MICITT) of Costa Rica. This work was funded by PND-018-15-2 from PINN POSGRADOS Ministerio de Ciencia, Tecnología y Telecomunicaciones (MICITT), a Doctoral scholarship for RM. Financial contribution to this study also came from 0174-10 CRIPAS, UNA Costa Rica and the Department of Population Health Sciences of Utrecht University. Funding Information: The authors acknowledge the contribution of the technical staff from the Virology laboratory EMV, UNA, and the Biosecurity Laboratory, LANASEVE, SENASA. Also, thanks go to Susana Ure?a for helping in sample collection and epidemiology data from farms used in this study. The authors thank Prof Hans Nauwynck and Boehringer Ingelheim for providing laboratory supplies and technical assistance and Sietske Ruijgh for the editing. Publisher Copyright: © 2021, The Author(s).

PY - 2021/12

Y1 - 2021/12

N2 - BACKGROUND: Worldwide, Porcine Reproductive and Respiratory Syndrome (PRRS) is among the diseases that cause the highest economic impact in modern pig production. PRRS was first detected in Costa Rica in 1996 and has since then severely affected the local swine industry. Studies of the molecular characterization of circulating strains, correlation with clinical records, and associations with pathogens associated with Porcine Respiratory Disease Complex (PRDC) have not been done in Costa Rica.RESULTS: Sequencing and phylogenetic analysis of ORF5 proved that PRRSV-2 was the only species detected in all locations analyzed. These sequences were grouped into three clusters. When comparing samples from San Jose, Alejuela, and Puntarenas to historical isolates of the previously described lineages (1 to 9), it has been shown that these were closely related to each other and belonged to Lineage 5, along with the samples from Heredia. Intriguingly, samples from Cartago clustered in a separate clade, phylogenetically related to Lineage 1. Epitope analysis conducted on the GP5 sequence of field isolates from Costa Rica revealed seven peptides with at least 80% amino acid sequence identity with previously described and experimentally validated immunogenic regions. Previously described epitopes A, B, and C, were detected in the Santa Barbara-Heredia isolate.CONCLUSIONS: Our data suggest that the virus has three distinct origins or introductions to the country. Future studies will elucidate how recently introduced vaccines will shape the evolutionary change of circulating field strains.

AB - BACKGROUND: Worldwide, Porcine Reproductive and Respiratory Syndrome (PRRS) is among the diseases that cause the highest economic impact in modern pig production. PRRS was first detected in Costa Rica in 1996 and has since then severely affected the local swine industry. Studies of the molecular characterization of circulating strains, correlation with clinical records, and associations with pathogens associated with Porcine Respiratory Disease Complex (PRDC) have not been done in Costa Rica.RESULTS: Sequencing and phylogenetic analysis of ORF5 proved that PRRSV-2 was the only species detected in all locations analyzed. These sequences were grouped into three clusters. When comparing samples from San Jose, Alejuela, and Puntarenas to historical isolates of the previously described lineages (1 to 9), it has been shown that these were closely related to each other and belonged to Lineage 5, along with the samples from Heredia. Intriguingly, samples from Cartago clustered in a separate clade, phylogenetically related to Lineage 1. Epitope analysis conducted on the GP5 sequence of field isolates from Costa Rica revealed seven peptides with at least 80% amino acid sequence identity with previously described and experimentally validated immunogenic regions. Previously described epitopes A, B, and C, were detected in the Santa Barbara-Heredia isolate.CONCLUSIONS: Our data suggest that the virus has three distinct origins or introductions to the country. Future studies will elucidate how recently introduced vaccines will shape the evolutionary change of circulating field strains.

KW - Costa Rica

KW - Glycoprotein 5(GP5)

KW - Open reading frame 5 (ORF5)

KW - Porcine Reproductive and Respiratory Syndrome (PRRS)

KW - Porcine Respiratory Disease Complex (PRDC)

KW - phylogenetic tree

UR - https://www.scopus.com/pages/publications/85107853548

U2 - 10.1186/s12917-021-02925-7

DO - 10.1186/s12917-021-02925-7

M3 - Article

C2 - 34118903

SN - 1746-6148

VL - 17

SP - 1

EP - 11

JO - BMC Veterinary Research

JF - BMC Veterinary Research

IS - 1

M1 - 217

ER -

Analysis of ORF5 sequences of Porcine Reproductive and Respiratory Syndrome virus (PRRSV) circulating within swine farms in Costa Rica

Abstract

Bibliographical note

UN SDGs

Keywords

Access to Document

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