Analysis of Neutral Oligosaccharides by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Bernd Stahl; Martin Steup; Michael Karas; Franz Hillenkamp

doi:10.1021/ac00014a022

Analysis of Neutral Oligosaccharides by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Bernd Stahl, Martin Steup, Michael Karas^*, Franz Hillenkamp

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Matrix-assisted laser desorption ionization mass spectrometry (LDI-MS) was used for mass determination of neutral nonderivatized oligosaccharides and mixtures thereof. Dihydroxybenzoic acid or caffeic acid served as matrix. A nitrogen laser (λ = 337 nm, τ = 5 ns) was used for ion desorption. For comparison ollgoglucans were analyzed by both high-performance and thin-layer chromatography. With LDI-MS glucan species were generally detected as monosodium and monopotassium carbohydrate adduct ions. Addition of lithium chloride to the analyte resulted In the formation of an additional monolithium glucan adduct ion. No fragmentation of the analyte was observed. LDI-MS as described here was also applied to analyze ollgofructans, mannose-containing oligosaccharides and glycolipids. LDI-MS has the advantages of being able to resolve complex carbohydrate mixtures, of high sensitivity and simplicity of analyte preparation; sample pretreatment, such as desalting is usually not required for LDI-MS.

Original language	English
Pages (from-to)	1463-1466
Number of pages	4
Journal	Analytical Chemistry
Volume	63
Issue number	14
DOIs	https://doi.org/10.1021/ac00014a022
Publication status	Published - 1 Jul 1991
Externally published	Yes

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title = "Analysis of Neutral Oligosaccharides by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry",

abstract = "Matrix-assisted laser desorption ionization mass spectrometry (LDI-MS) was used for mass determination of neutral nonderivatized oligosaccharides and mixtures thereof. Dihydroxybenzoic acid or caffeic acid served as matrix. A nitrogen laser (λ = 337 nm, τ = 5 ns) was used for ion desorption. For comparison ollgoglucans were analyzed by both high-performance and thin-layer chromatography. With LDI-MS glucan species were generally detected as monosodium and monopotassium carbohydrate adduct ions. Addition of lithium chloride to the analyte resulted In the formation of an additional monolithium glucan adduct ion. No fragmentation of the analyte was observed. LDI-MS as described here was also applied to analyze ollgofructans, mannose-containing oligosaccharides and glycolipids. LDI-MS has the advantages of being able to resolve complex carbohydrate mixtures, of high sensitivity and simplicity of analyte preparation; sample pretreatment, such as desalting is usually not required for LDI-MS.",

author = "Bernd Stahl and Martin Steup and Michael Karas and Franz Hillenkamp",

year = "1991",

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doi = "10.1021/ac00014a022",

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T1 - Analysis of Neutral Oligosaccharides by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

AU - Stahl, Bernd

AU - Steup, Martin

AU - Karas, Michael

AU - Hillenkamp, Franz

PY - 1991/7/1

Y1 - 1991/7/1

N2 - Matrix-assisted laser desorption ionization mass spectrometry (LDI-MS) was used for mass determination of neutral nonderivatized oligosaccharides and mixtures thereof. Dihydroxybenzoic acid or caffeic acid served as matrix. A nitrogen laser (λ = 337 nm, τ = 5 ns) was used for ion desorption. For comparison ollgoglucans were analyzed by both high-performance and thin-layer chromatography. With LDI-MS glucan species were generally detected as monosodium and monopotassium carbohydrate adduct ions. Addition of lithium chloride to the analyte resulted In the formation of an additional monolithium glucan adduct ion. No fragmentation of the analyte was observed. LDI-MS as described here was also applied to analyze ollgofructans, mannose-containing oligosaccharides and glycolipids. LDI-MS has the advantages of being able to resolve complex carbohydrate mixtures, of high sensitivity and simplicity of analyte preparation; sample pretreatment, such as desalting is usually not required for LDI-MS.

AB - Matrix-assisted laser desorption ionization mass spectrometry (LDI-MS) was used for mass determination of neutral nonderivatized oligosaccharides and mixtures thereof. Dihydroxybenzoic acid or caffeic acid served as matrix. A nitrogen laser (λ = 337 nm, τ = 5 ns) was used for ion desorption. For comparison ollgoglucans were analyzed by both high-performance and thin-layer chromatography. With LDI-MS glucan species were generally detected as monosodium and monopotassium carbohydrate adduct ions. Addition of lithium chloride to the analyte resulted In the formation of an additional monolithium glucan adduct ion. No fragmentation of the analyte was observed. LDI-MS as described here was also applied to analyze ollgofructans, mannose-containing oligosaccharides and glycolipids. LDI-MS has the advantages of being able to resolve complex carbohydrate mixtures, of high sensitivity and simplicity of analyte preparation; sample pretreatment, such as desalting is usually not required for LDI-MS.

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JO - Analytical Chemistry

JF - Analytical Chemistry

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ER -

Analysis of Neutral Oligosaccharides by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Abstract

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