Analysis of caffeine and paraxanthine in human saliva with ultra-high-performance liquid chromatography for CYP1A2 phenotyping

Nan Yeun Jordan; Jolet Y. Mimpen; Willie J. M. van den Bogaard; Frits M. Flesch; Michiel H. M. van de Meent; Javier Sastre Torano

doi:10.1016/j.jchromb.2015.05.020

Analysis of caffeine and paraxanthine in human saliva with ultra-high-performance liquid chromatography for CYP1A2 phenotyping

Nan Yeun Jordan, Jolet Y. Mimpen, Willie J. M. van den Bogaard, Frits M. Flesch, Michiel H. M. van de Meent, Javier Sastre Torano^*

^*Corresponding author for this work

Sub Onderwijsinstituut Farmacie

Utrecht University

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Cytochrome P450 1A2 (CYP1A2) plays an important role in drug metabolism. Caffeine (CAF) is converted into paraxanthine (PX) by this enzyme and is used as a xenobiotic substrate to determine the CYP1A2 phenotype in humans. A method for the quantification of CAF and PX in saliva was developed using liquid-liquid extraction with ethyl acetate and analysis with ultra-high-performance liquid chromatography. Peaks from CAF, PX and internal standard were resolved within 6 min. The method was validated from 0.05 to 5 mu g mL(-1) CAF and 0.025-2.5 mu g mL(-1) PX. Inter- and intra-day accuracies ranged from 91.2 to 107.2% with precisions

Original language	English
Pages (from-to)	70-73
Number of pages	4
Journal	Journal of chromatography. B
Volume	995
DOIs	https://doi.org/10.1016/j.jchromb.2015.05.020
Publication status	Published - 15 Jul 2015

Keywords

Caffeine
Paraxanthine
CYP1A2
Phenotyping
Saliva
UHPLC
IN-VIVO
PLASMA
THEOPHYLLINE
METABOLITES
CYP2E1
RATIO
DRUG

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10.1016/j.jchromb.2015.05.020

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@article{f2ee0ced66ee4918b071145e027902bc,

title = "Analysis of caffeine and paraxanthine in human saliva with ultra-high-performance liquid chromatography for CYP1A2 phenotyping",

abstract = "Cytochrome P450 1A2 (CYP1A2) plays an important role in drug metabolism. Caffeine (CAF) is converted into paraxanthine (PX) by this enzyme and is used as a xenobiotic substrate to determine the CYP1A2 phenotype in humans. A method for the quantification of CAF and PX in saliva was developed using liquid-liquid extraction with ethyl acetate and analysis with ultra-high-performance liquid chromatography. Peaks from CAF, PX and internal standard were resolved within 6 min. The method was validated from 0.05 to 5 mu g mL(-1) CAF and 0.025-2.5 mu g mL(-1) PX. Inter- and intra-day accuracies ranged from 91.2 to 107.2% with precisions",

keywords = "Caffeine, Paraxanthine, CYP1A2, Phenotyping, Saliva, UHPLC, IN-VIVO, PLASMA, THEOPHYLLINE, METABOLITES, CYP2E1, RATIO, DRUG",

author = "Jordan, {Nan Yeun} and Mimpen, {Jolet Y.} and {van den Bogaard}, {Willie J. M.} and Flesch, {Frits M.} and {van de Meent}, {Michiel H. M.} and Torano, {Javier Sastre}",

year = "2015",

month = jul,

day = "15",

doi = "10.1016/j.jchromb.2015.05.020",

language = "English",

volume = "995",

pages = "70--73",

journal = "Journal of chromatography. B",

issn = "1570-0232",

publisher = "Elsevier",

}

TY - JOUR

T1 - Analysis of caffeine and paraxanthine in human saliva with ultra-high-performance liquid chromatography for CYP1A2 phenotyping

AU - Jordan, Nan Yeun

AU - Mimpen, Jolet Y.

AU - van den Bogaard, Willie J. M.

AU - Flesch, Frits M.

AU - van de Meent, Michiel H. M.

AU - Torano, Javier Sastre

PY - 2015/7/15

Y1 - 2015/7/15

N2 - Cytochrome P450 1A2 (CYP1A2) plays an important role in drug metabolism. Caffeine (CAF) is converted into paraxanthine (PX) by this enzyme and is used as a xenobiotic substrate to determine the CYP1A2 phenotype in humans. A method for the quantification of CAF and PX in saliva was developed using liquid-liquid extraction with ethyl acetate and analysis with ultra-high-performance liquid chromatography. Peaks from CAF, PX and internal standard were resolved within 6 min. The method was validated from 0.05 to 5 mu g mL(-1) CAF and 0.025-2.5 mu g mL(-1) PX. Inter- and intra-day accuracies ranged from 91.2 to 107.2% with precisions

AB - Cytochrome P450 1A2 (CYP1A2) plays an important role in drug metabolism. Caffeine (CAF) is converted into paraxanthine (PX) by this enzyme and is used as a xenobiotic substrate to determine the CYP1A2 phenotype in humans. A method for the quantification of CAF and PX in saliva was developed using liquid-liquid extraction with ethyl acetate and analysis with ultra-high-performance liquid chromatography. Peaks from CAF, PX and internal standard were resolved within 6 min. The method was validated from 0.05 to 5 mu g mL(-1) CAF and 0.025-2.5 mu g mL(-1) PX. Inter- and intra-day accuracies ranged from 91.2 to 107.2% with precisions

KW - Caffeine

KW - Paraxanthine

KW - CYP1A2

KW - Phenotyping

KW - Saliva

KW - UHPLC

KW - IN-VIVO

KW - PLASMA

KW - THEOPHYLLINE

KW - METABOLITES

KW - CYP2E1

KW - RATIO

KW - DRUG

U2 - 10.1016/j.jchromb.2015.05.020

DO - 10.1016/j.jchromb.2015.05.020

M3 - Article

SN - 1570-0232

VL - 995

SP - 70

EP - 73

JO - Journal of chromatography. B

JF - Journal of chromatography. B

ER -

Analysis of caffeine and paraxanthine in human saliva with ultra-high-performance liquid chromatography for CYP1A2 phenotyping

Abstract

Keywords

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