An enzyme cocktail for efficient protoplast formation in Aspergillus niger

Charissa de Bekker; Ad Wiebenga; Guillermo Aguilar; Han A.B. Wösten

doi:10.1016/j.mimet.2008.11.001

An enzyme cocktail for efficient protoplast formation in Aspergillus niger

Charissa de Bekker, Ad Wiebenga, Guillermo Aguilar, Han A.B. Wösten^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Novozym 234 has been traditionally used to prepare protoplasts for genetic transformation of fungi. Since it is no longer on the market, a new enzyme cocktail was defined to protoplast Aspergillus niger. The cocktail consists of lysing enzymes from Trichoderma harzianum, chitinase from Streptomyces griseus and β-glucuronidase from Helix pomatia.

Original language	English
Pages (from-to)	305-306
Number of pages	2
Journal	Journal of Microbiological Methods
Volume	76
Issue number	3
DOIs	https://doi.org/10.1016/j.mimet.2008.11.001
Publication status	Published - 1 Mar 2009

Funding

This work was supported by The Netherlands Technology Foundation (STW) project 7453.

Keywords

Aspergillus niger
Fungi
Protoplast formation
Protoplast regeneration
Protoplast storage
Protoplast transformation

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10.1016/j.mimet.2008.11.001

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title = "An enzyme cocktail for efficient protoplast formation in Aspergillus niger",

abstract = "Novozym 234 has been traditionally used to prepare protoplasts for genetic transformation of fungi. Since it is no longer on the market, a new enzyme cocktail was defined to protoplast Aspergillus niger. The cocktail consists of lysing enzymes from Trichoderma harzianum, chitinase from Streptomyces griseus and β-glucuronidase from Helix pomatia.",

keywords = "Aspergillus niger, Fungi, Protoplast formation, Protoplast regeneration, Protoplast storage, Protoplast transformation",

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AU - Wiebenga, Ad

AU - Aguilar, Guillermo

AU - Wösten, Han A.B.

PY - 2009/3/1

Y1 - 2009/3/1

N2 - Novozym 234 has been traditionally used to prepare protoplasts for genetic transformation of fungi. Since it is no longer on the market, a new enzyme cocktail was defined to protoplast Aspergillus niger. The cocktail consists of lysing enzymes from Trichoderma harzianum, chitinase from Streptomyces griseus and β-glucuronidase from Helix pomatia.

AB - Novozym 234 has been traditionally used to prepare protoplasts for genetic transformation of fungi. Since it is no longer on the market, a new enzyme cocktail was defined to protoplast Aspergillus niger. The cocktail consists of lysing enzymes from Trichoderma harzianum, chitinase from Streptomyces griseus and β-glucuronidase from Helix pomatia.

KW - Aspergillus niger

KW - Fungi

KW - Protoplast formation

KW - Protoplast regeneration

KW - Protoplast storage

KW - Protoplast transformation

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JF - Journal of Microbiological Methods

IS - 3

ER -

An enzyme cocktail for efficient protoplast formation in Aspergillus niger

Abstract

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Keywords

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