An enzyme cocktail for efficient protoplast formation in Aspergillus niger

Charissa de Bekker, Ad Wiebenga, Guillermo Aguilar, Han A.B. Wösten*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Novozym 234 has been traditionally used to prepare protoplasts for genetic transformation of fungi. Since it is no longer on the market, a new enzyme cocktail was defined to protoplast Aspergillus niger. The cocktail consists of lysing enzymes from Trichoderma harzianum, chitinase from Streptomyces griseus and β-glucuronidase from Helix pomatia.

Original languageEnglish
Pages (from-to)305-306
Number of pages2
JournalJournal of Microbiological Methods
Volume76
Issue number3
DOIs
Publication statusPublished - 1 Mar 2009

Keywords

  • Aspergillus niger
  • Fungi
  • Protoplast formation
  • Protoplast regeneration
  • Protoplast storage
  • Protoplast transformation

Fingerprint

Dive into the research topics of 'An enzyme cocktail for efficient protoplast formation in Aspergillus niger'. Together they form a unique fingerprint.

Cite this