Adhesion molecules: a new target for immunoliposome-mediated drug delivery

P. G.M. Bloemen; P. A.J. Henricks; L. van Bloois; M. C. van den Tweel; A. C. Bloem; F. P. Nijkamp; D. J.A. Crommelin; G. Storm

doi:10.1016/0014-5793(94)01350-A

Adhesion molecules: a new target for immunoliposome-mediated drug delivery

P. G.M. Bloemen^*
, P. A.J. Henricks
, L. van Bloois
, M. C. van den Tweel
, A. C. Bloem
, F. P. Nijkamp
, D. J.A. Crommelin
, G. Storm

^*Corresponding author for this work

Utrecht University

Research output: Contribution to journal › Letter › Academic › peer-review

Abstract

The anti-ICAM-1 monoclonal antibody F10.2 was conjugated to liposomes to target to cells expressing the cell adhesion molecule ICAM-1. We demonstrate that F10.2 immunoliposomes bind to human bronchial epithelial cells (BEAS-2B) and human umbilical vein endothelial cells (HUVEC) in a specific, dose- and time-dependent manner. It appears that the degree of ICAM-1 expression is the limiting factor in the degree of immunoliposome binding to the cells. These results are a first step in the strategy for specific drug delivery to target sites characterised by increased expression of adhesion molecules.

Original language	English
Pages (from-to)	140-144
Number of pages	5
Journal	FEBS Letters
Volume	357
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(94)01350-A
Publication status	Published - 3 Jan 1995

Bibliographical note

Funding Information:
Acknowledgments This work was supported in part by the Dutch Asthma Foundation

Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.

Funding

Acknowledgments This work was supported in part by the Dutch Asthma Foundation

Keywords

bovine serum albumine
BSA
CHOL
cholesterol
dimethylformamide
DMF
EDTA
egg-phosphatidylglycerol
Endothelium
EPG
Epithelium
ethylene diaminetetraacetic acid
FCS
fluorescein isothiocyanate-labeled goat anti-mouse mAb
foetal calf serum
GamFITC
HBS
HEPES-buffered saline
human umbilical vein endothelial cells
HUVEC
ICAM-1
IFN-γ
Immunoliposome
intercellular adhesion molecule-1
interferon-γ
mAb
mean fluorescence intensity
MFI
Monoclonal antibody
monoclonal antibody
MPB-PE
N-succinimidyl S-acetylthioacetate
N-[4-(p-maleimidophenyl)butyryl] phosphatidylethanolamine
partially hydrogenated egg l-α-phosphatidylcholine
PBS
PHEPC
phosphate-buffered saline
phospholipid
PL
SATA
TNF-α
tumor necrosis factor-α
vascular cell adhesion molecule-1
VCAM-1

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10.1016/0014-5793(94)01350-A

FEBS Letters - January 03 1995 - Bloemen - Adhesion molecules a new target for immunoliposome‐mediated drug deliveryFinal published version, 543 KBLicence: Taverne

Cite this

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title = "Adhesion molecules: a new target for immunoliposome-mediated drug delivery",

abstract = "The anti-ICAM-1 monoclonal antibody F10.2 was conjugated to liposomes to target to cells expressing the cell adhesion molecule ICAM-1. We demonstrate that F10.2 immunoliposomes bind to human bronchial epithelial cells (BEAS-2B) and human umbilical vein endothelial cells (HUVEC) in a specific, dose- and time-dependent manner. It appears that the degree of ICAM-1 expression is the limiting factor in the degree of immunoliposome binding to the cells. These results are a first step in the strategy for specific drug delivery to target sites characterised by increased expression of adhesion molecules.",

keywords = "bovine serum albumine, BSA, CHOL, cholesterol, dimethylformamide, DMF, EDTA, egg-phosphatidylglycerol, Endothelium, EPG, Epithelium, ethylene diaminetetraacetic acid, FCS, fluorescein isothiocyanate-labeled goat anti-mouse mAb, foetal calf serum, GamFITC, HBS, HEPES-buffered saline, human umbilical vein endothelial cells, HUVEC, ICAM-1, IFN-γ, Immunoliposome, intercellular adhesion molecule-1, interferon-γ, mAb, mean fluorescence intensity, MFI, Monoclonal antibody, monoclonal antibody, MPB-PE, N-succinimidyl S-acetylthioacetate, N-[4-(p-maleimidophenyl)butyryl] phosphatidylethanolamine, partially hydrogenated egg l-α-phosphatidylcholine, PBS, PHEPC, phosphate-buffered saline, phospholipid, PL, SATA, TNF-α, tumor necrosis factor-α, vascular cell adhesion molecule-1, VCAM-1",

author = "Bloemen, \{P. G.M.\} and Henricks, \{P. A.J.\} and \{van Bloois\}, L. and \{van den Tweel\}, \{M. C.\} and Bloem, \{A. C.\} and Nijkamp, \{F. P.\} and Crommelin, \{D. J.A.\} and G. Storm",

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doi = "10.1016/0014-5793(94)01350-A",

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AU - Bloemen, P. G.M.

AU - Henricks, P. A.J.

AU - van Bloois, L.

AU - van den Tweel, M. C.

AU - Bloem, A. C.

AU - Nijkamp, F. P.

AU - Crommelin, D. J.A.

AU - Storm, G.

PY - 1995/1/3

Y1 - 1995/1/3

N2 - The anti-ICAM-1 monoclonal antibody F10.2 was conjugated to liposomes to target to cells expressing the cell adhesion molecule ICAM-1. We demonstrate that F10.2 immunoliposomes bind to human bronchial epithelial cells (BEAS-2B) and human umbilical vein endothelial cells (HUVEC) in a specific, dose- and time-dependent manner. It appears that the degree of ICAM-1 expression is the limiting factor in the degree of immunoliposome binding to the cells. These results are a first step in the strategy for specific drug delivery to target sites characterised by increased expression of adhesion molecules.

AB - The anti-ICAM-1 monoclonal antibody F10.2 was conjugated to liposomes to target to cells expressing the cell adhesion molecule ICAM-1. We demonstrate that F10.2 immunoliposomes bind to human bronchial epithelial cells (BEAS-2B) and human umbilical vein endothelial cells (HUVEC) in a specific, dose- and time-dependent manner. It appears that the degree of ICAM-1 expression is the limiting factor in the degree of immunoliposome binding to the cells. These results are a first step in the strategy for specific drug delivery to target sites characterised by increased expression of adhesion molecules.

KW - bovine serum albumine

KW - BSA

KW - CHOL

KW - cholesterol

KW - dimethylformamide

KW - DMF

KW - EDTA

KW - egg-phosphatidylglycerol

KW - Endothelium

KW - EPG

KW - Epithelium

KW - ethylene diaminetetraacetic acid

KW - FCS

KW - fluorescein isothiocyanate-labeled goat anti-mouse mAb

KW - foetal calf serum

KW - GamFITC

KW - HBS

KW - HEPES-buffered saline

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KW - HUVEC

KW - ICAM-1

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KW - intercellular adhesion molecule-1

KW - interferon-γ

KW - mAb

KW - mean fluorescence intensity

KW - MFI

KW - Monoclonal antibody

KW - monoclonal antibody

KW - MPB-PE

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KW - N-[4-(p-maleimidophenyl)butyryl] phosphatidylethanolamine

KW - partially hydrogenated egg l-α-phosphatidylcholine

KW - PBS

KW - PHEPC

KW - phosphate-buffered saline

KW - phospholipid

KW - PL

KW - SATA

KW - TNF-α

KW - tumor necrosis factor-α

KW - vascular cell adhesion molecule-1

KW - VCAM-1

UR - https://www.scopus.com/pages/publications/0028878566

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DO - 10.1016/0014-5793(94)01350-A

M3 - Letter

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AN - SCOPUS:0028878566

SN - 0014-5793

VL - 357

SP - 140

EP - 144

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Adhesion molecules: a new target for immunoliposome-mediated drug delivery

Abstract

Bibliographical note

Funding

Keywords

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