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L. Vervelde; M.G.R. Matthijs; D.A. van Haarlem; Sjaak de Wit; C.A. Jansen

doi:10.1016/j.vetimm.2012.11.012

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L. Vervelde, M.G.R. Matthijs, D.A. van Haarlem, Sjaak de Wit, C.A. Jansen

Animal Health Service Deventer

Research output: Contribution to journal › Article › Academic

Abstract

Natural killer (NK) cells are cytotoxic lymphocytes and play an important role in the early
defence against viruses. In this study we focussed on NK cell and interferon (IFN) responses
after infection with infectious bronchitis virus (IBV). Based on surface expression of CD107+,
enhanced activation of lung NK cells was observed at 1 dpi, whereas in blood prolonged NKcell
activation was found. IFN- and IFN- mRNA and proteins were not rapidly induced
whereas IFN- production in lung, measured by Elispot assay, increased over time at 2 and
4 dpi. In contrast, IFN- production in blood was highest at 1 dpi and decreased over time
down to levels comparable to uninfected birds at 4 dpi. Collectively, infection with IBV-M41
resulted in activation of NK cells in the lung and blood and rapid production of IFN- and
not IFN- and IFN- compared to uninfected birds.

Original language	English
Pages (from-to)	337-341
Journal	Veterinary Immunology and Immunopathology
Volume	151
DOIs	https://doi.org/10.1016/j.vetimm.2012.11.012
Publication status	Published - 2013

Keywords

Infectious bronchitis virus
Natural killer cells
Interferon
Mucosal immunity

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10.1016/j.vetimm.2012.11.012

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Vervelde et al vet immunol immunopathol
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title = "add electronic version",

abstract = "Natural killer (NK) cells are cytotoxic lymphocytes and play an important role in the earlydefence against viruses. In this study we focussed on NK cell and interferon (IFN) responsesafter infection with infectious bronchitis virus (IBV). Based on surface expression of CD107+,enhanced activation of lung NK cells was observed at 1 dpi, whereas in blood prolonged NKcellactivation was found. IFN- and IFN- mRNA and proteins were not rapidly inducedwhereas IFN- production in lung, measured by Elispot assay, increased over time at 2 and4 dpi. In contrast, IFN- production in blood was highest at 1 dpi and decreased over timedown to levels comparable to uninfected birds at 4 dpi. Collectively, infection with IBV-M41resulted in activation of NK cells in the lung and blood and rapid production of IFN- andnot IFN- and IFN- compared to uninfected birds.",

keywords = "Infectious bronchitis virus, Natural killer cells, Interferon, Mucosal immunity",

author = "L. Vervelde and M.G.R. Matthijs and {van Haarlem}, D.A. and {de Wit}, Sjaak and C.A. Jansen",

year = "2013",

doi = "10.1016/j.vetimm.2012.11.012",

language = "English",

volume = "151",

pages = "337--341",

journal = "Veterinary Immunology and Immunopathology",

issn = "0165-2427",

publisher = "Elsevier",

}

TY - JOUR

T1 - add electronic version

AU - Vervelde, L.

AU - Matthijs, M.G.R.

AU - van Haarlem, D.A.

AU - de Wit, Sjaak

AU - Jansen, C.A.

PY - 2013

Y1 - 2013

N2 - Natural killer (NK) cells are cytotoxic lymphocytes and play an important role in the earlydefence against viruses. In this study we focussed on NK cell and interferon (IFN) responsesafter infection with infectious bronchitis virus (IBV). Based on surface expression of CD107+,enhanced activation of lung NK cells was observed at 1 dpi, whereas in blood prolonged NKcellactivation was found. IFN- and IFN- mRNA and proteins were not rapidly inducedwhereas IFN- production in lung, measured by Elispot assay, increased over time at 2 and4 dpi. In contrast, IFN- production in blood was highest at 1 dpi and decreased over timedown to levels comparable to uninfected birds at 4 dpi. Collectively, infection with IBV-M41resulted in activation of NK cells in the lung and blood and rapid production of IFN- andnot IFN- and IFN- compared to uninfected birds.

AB - Natural killer (NK) cells are cytotoxic lymphocytes and play an important role in the earlydefence against viruses. In this study we focussed on NK cell and interferon (IFN) responsesafter infection with infectious bronchitis virus (IBV). Based on surface expression of CD107+,enhanced activation of lung NK cells was observed at 1 dpi, whereas in blood prolonged NKcellactivation was found. IFN- and IFN- mRNA and proteins were not rapidly inducedwhereas IFN- production in lung, measured by Elispot assay, increased over time at 2 and4 dpi. In contrast, IFN- production in blood was highest at 1 dpi and decreased over timedown to levels comparable to uninfected birds at 4 dpi. Collectively, infection with IBV-M41resulted in activation of NK cells in the lung and blood and rapid production of IFN- andnot IFN- and IFN- compared to uninfected birds.

KW - Infectious bronchitis virus

KW - Natural killer cells

KW - Interferon

KW - Mucosal immunity

U2 - 10.1016/j.vetimm.2012.11.012

DO - 10.1016/j.vetimm.2012.11.012

M3 - Article

SN - 0165-2427

VL - 151

SP - 337

EP - 341

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

ER -

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