Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains

Shih-Te Hung; Arnau Llobet Rosell; Daphne Jurriens; Marijn Siemons; Oleg Soloviev; Lukas C Kapitein; Kristin Grußmayer; Lukas J Neukomm; Michel Verhaegen; Carlas Smith

doi:10.3389/fnins.2022.954949

Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains

Shih-Te Hung, Arnau Llobet Rosell, Daphne Jurriens, Marijn Siemons, Oleg Soloviev, Lukas C Kapitein, Kristin Grußmayer, Lukas J Neukomm, Michel Verhaegen, Carlas Smith

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort the point spread function (PSF), and high background fluorescence decreases the localization precision. Here, we synergistically combine sensorless adaptive optics (AO), in-situ 3D-PSF calibration, and a single-objective lens inclined light sheet microscope (SOLEIL), termed (AO-SOLEIL), to mitigate deep tissue-induced deteriorations. We apply AO-SOLEIL on several dSTORM samples including brains of adult Drosophila. We observed a 2x improvement in the estimated axial localization precision with respect to widefield without aberration correction while we used synergistic solution. AO-SOLEIL enhances the overall imaging resolution and further facilitates the visualization of sub-cellular structures in tissue.

Original language	English
Article number	954949
Pages (from-to)	1-21
Journal	Frontiers in Neuroscience
Volume	16
DOIs	https://doi.org/10.3389/fnins.2022.954949
Publication status	Published - 6 Oct 2022

Bibliographical note

Keywords

Super-resolution Microscopy
localization microscopy
adaptive optics
Drosophila
brain

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Hung, S.-T., Llobet Rosell, A., Jurriens, D., Siemons, M., Soloviev, O., Kapitein, L. C., Grußmayer, K., Neukomm, L. J., Verhaegen, M., & Smith, C. (2022). Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains. Frontiers in Neuroscience, 16, 1-21. Article 954949. https://doi.org/10.3389/fnins.2022.954949

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title = "Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains",

abstract = "Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort the point spread function (PSF), and high background fluorescence decreases the localization precision. Here, we synergistically combine sensorless adaptive optics (AO), in-situ 3D-PSF calibration, and a single-objective lens inclined light sheet microscope (SOLEIL), termed (AO-SOLEIL), to mitigate deep tissue-induced deteriorations. We apply AO-SOLEIL on several dSTORM samples including brains of adult Drosophila. We observed a 2x improvement in the estimated axial localization precision with respect to widefield without aberration correction while we used synergistic solution. AO-SOLEIL enhances the overall imaging resolution and further facilitates the visualization of sub-cellular structures in tissue.",

keywords = "Super-resolution Microscopy, localization microscopy, adaptive optics, Drosophila, brain",

author = "Shih-Te Hung and \{Llobet Rosell\}, Arnau and Daphne Jurriens and Marijn Siemons and Oleg Soloviev and Kapitein, \{Lukas C\} and Kristin Gru{\ss}mayer and Neukomm, \{Lukas J\} and Michel Verhaegen and Carlas Smith",

note = "Copyright {\textcopyright} 2022 Hung, Llobet Rosell, Jurriens, Siemons, Soloviev, Kapitein, Gru{\ss}mayer, Neukomm, Verhaegen and Smith.",

year = "2022",

month = oct,

day = "6",

doi = "10.3389/fnins.2022.954949",

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Hung, S-T, Llobet Rosell, A, Jurriens, D, Siemons, M, Soloviev, O, Kapitein, LC, Grußmayer, K, Neukomm, LJ, Verhaegen, M & Smith, C 2022, 'Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains', Frontiers in Neuroscience, vol. 16, 954949, pp. 1-21. https://doi.org/10.3389/fnins.2022.954949

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T1 - Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains

AU - Hung, Shih-Te

AU - Llobet Rosell, Arnau

AU - Jurriens, Daphne

AU - Siemons, Marijn

AU - Soloviev, Oleg

AU - Kapitein, Lukas C

AU - Grußmayer, Kristin

AU - Neukomm, Lukas J

AU - Verhaegen, Michel

AU - Smith, Carlas

PY - 2022/10/6

Y1 - 2022/10/6

N2 - Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort the point spread function (PSF), and high background fluorescence decreases the localization precision. Here, we synergistically combine sensorless adaptive optics (AO), in-situ 3D-PSF calibration, and a single-objective lens inclined light sheet microscope (SOLEIL), termed (AO-SOLEIL), to mitigate deep tissue-induced deteriorations. We apply AO-SOLEIL on several dSTORM samples including brains of adult Drosophila. We observed a 2x improvement in the estimated axial localization precision with respect to widefield without aberration correction while we used synergistic solution. AO-SOLEIL enhances the overall imaging resolution and further facilitates the visualization of sub-cellular structures in tissue.

AB - Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort the point spread function (PSF), and high background fluorescence decreases the localization precision. Here, we synergistically combine sensorless adaptive optics (AO), in-situ 3D-PSF calibration, and a single-objective lens inclined light sheet microscope (SOLEIL), termed (AO-SOLEIL), to mitigate deep tissue-induced deteriorations. We apply AO-SOLEIL on several dSTORM samples including brains of adult Drosophila. We observed a 2x improvement in the estimated axial localization precision with respect to widefield without aberration correction while we used synergistic solution. AO-SOLEIL enhances the overall imaging resolution and further facilitates the visualization of sub-cellular structures in tissue.

KW - Super-resolution Microscopy

KW - localization microscopy

KW - adaptive optics

KW - Drosophila

KW - brain

U2 - 10.3389/fnins.2022.954949

DO - 10.3389/fnins.2022.954949

M3 - Article

C2 - 36278016

SN - 1662-4548

VL - 16

SP - 1

EP - 21

JO - Frontiers in Neuroscience

JF - Frontiers in Neuroscience

M1 - 954949

ER -

Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains

Abstract

Bibliographical note

Keywords

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