Activated CD4+T cells enter the splenic T-cell zone and induce autoantibody-producing germinal centers through bystander activation

CD4+T (helper) cells migrate in huge numbers through lymphoid organs. However, little is known about traffic routes and kinetics of CD4+T-cell subsets within different organ compartments. Such information is important because there are indications that CD4+T cells may influence the function of microenvironments depending on their developmental stage. Therefore, we investigated the migration of resting (naïve), activated, and recently activated (memory) CD4+T cells through the different compartments of the spleen. Resting and recently activated CD4+T cells were separated from thoracic duct lymph and activated CD4+T cells were generated in vitro by cross-linking the T-cell receptor and CD28. The present study shows that all three CD4+T-cell subsets selectively accumulate in the T-cell zone of the spleen. However, only activated T cells induce the formation of germinal centers (GCs) and autoantibodies in rats and mice. Our results suggest that in a two-step process they first activate B cells independent of the T-cell receptor repertoire and CD40 ligand (CD154) expression. The activated B cells then form GCs whereby CD154-dependend T-cell help is needed. Thus, activated T cells may contribute to the development of autoimmune diseases by activating autoreactive B cells in an Ag-independent manner. European Journal of Immunology published by Wiley-VCH Verlag GmbH & Co. KGaA Weinheim.