Accurate molecular weight analysis of histones using FFE and RP-HPLC on monolithic capillary columns

E.J. Sneekes; J. Han; M. Elliot; J. Ausio; R. Swart; A.J.R. Heck; C. Borchers

doi:10.1002/jssc.200800627

Accurate molecular weight analysis of histones using FFE and RP-HPLC on monolithic capillary columns

E.J. Sneekes, J. Han, M. Elliot, J. Ausio, R. Swart, A.J.R. Heck, C. Borchers

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Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Due to their large diversity with respect to post‐translational modifications (PTMs), the family of histones provides a major analytical challenge in current proteomics research. Their function has a large impact on the transcription of DNA, and as a result, on the expression of proteins. The variation in PTMs regulates transcription, and, as a result, many methods are being employed for the in‐depth analysis of histones. In this paper, we present a separation strategy for histones based on free‐flow electrophoresis (FFE) followed by an RP separation on capillary monolithic PS‐DVB columns. The capillary columns are directly interfaced with an FT‐ICR MS providing an online system for the detection and accurate molecular weight analysis of intact histones.

Original language	English
Pages (from-to)	2691-2698
Number of pages	8
Journal	Journal of Separation Science
Volume	32
Issue number	16/16
DOIs	https://doi.org/10.1002/jssc.200800627
Publication status	Published - 2009

Keywords

Farmacie/Biofarmaceutische wetenschappen (FARM)
Farmacie(FARM)

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10.1002/jssc.200800627

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title = "Accurate molecular weight analysis of histones using FFE and RP-HPLC on monolithic capillary columns",

abstract = "Due to their large diversity with respect to post‐translational modifications (PTMs), the family of histones provides a major analytical challenge in current proteomics research. Their function has a large impact on the transcription of DNA, and as a result, on the expression of proteins. The variation in PTMs regulates transcription, and, as a result, many methods are being employed for the in‐depth analysis of histones. In this paper, we present a separation strategy for histones based on free‐flow electrophoresis (FFE) followed by an RP separation on capillary monolithic PS‐DVB columns. The capillary columns are directly interfaced with an FT‐ICR MS providing an online system for the detection and accurate molecular weight analysis of intact histones.",

keywords = "Farmacie/Biofarmaceutische wetenschappen (FARM), Farmacie(FARM)",

author = "E.J. Sneekes and J. Han and M. Elliot and J. Ausio and R. Swart and A.J.R. Heck and C. Borchers",

year = "2009",

doi = "10.1002/jssc.200800627",

language = "English",

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TY - JOUR

T1 - Accurate molecular weight analysis of histones using FFE and RP-HPLC on monolithic capillary columns

AU - Sneekes, E.J.

AU - Han, J.

AU - Elliot, M.

AU - Ausio, J.

AU - Swart, R.

AU - Heck, A.J.R.

AU - Borchers, C.

PY - 2009

Y1 - 2009

N2 - Due to their large diversity with respect to post‐translational modifications (PTMs), the family of histones provides a major analytical challenge in current proteomics research. Their function has a large impact on the transcription of DNA, and as a result, on the expression of proteins. The variation in PTMs regulates transcription, and, as a result, many methods are being employed for the in‐depth analysis of histones. In this paper, we present a separation strategy for histones based on free‐flow electrophoresis (FFE) followed by an RP separation on capillary monolithic PS‐DVB columns. The capillary columns are directly interfaced with an FT‐ICR MS providing an online system for the detection and accurate molecular weight analysis of intact histones.

AB - Due to their large diversity with respect to post‐translational modifications (PTMs), the family of histones provides a major analytical challenge in current proteomics research. Their function has a large impact on the transcription of DNA, and as a result, on the expression of proteins. The variation in PTMs regulates transcription, and, as a result, many methods are being employed for the in‐depth analysis of histones. In this paper, we present a separation strategy for histones based on free‐flow electrophoresis (FFE) followed by an RP separation on capillary monolithic PS‐DVB columns. The capillary columns are directly interfaced with an FT‐ICR MS providing an online system for the detection and accurate molecular weight analysis of intact histones.

KW - Farmacie/Biofarmaceutische wetenschappen (FARM)

KW - Farmacie(FARM)

U2 - 10.1002/jssc.200800627

DO - 10.1002/jssc.200800627

M3 - Article

SN - 1615-9306

VL - 32

SP - 2691

EP - 2698

JO - Journal of Separation Science

JF - Journal of Separation Science

IS - 16/16

ER -

Accurate molecular weight analysis of histones using FFE and RP-HPLC on monolithic capillary columns

Abstract

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