Accurate molecular mass determination by mass spectrometrical techniques: Differentiation of soybean lipoxygenase isoenzymes

G. Scheller; B. Stahl; S. Weyd; M. Karas; F. Hillenkamp; P. Schreier

doi:10.1002/pca.2800050602

Accurate molecular mass determination by mass spectrometrical techniques: Differentiation of soybean lipoxygenase isoenzymes

G. Scheller, B. Stahl, S. Weyd, M. Karas, F. Hillenkamp, P. Schreier^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Molecular mass determinations for the four lipoxygenase isoenzymes from soybean seeds were compared using two mass spectrometrical (MS) techniques as well as gel permeation chromatography and sodium dodecylsulphate‐polyacrylamide gel electrophoresis. The data obtained by MS techniques were in good agreement with the molecular masses evaluated from the corresponding cDNA. The MS techniques allowed to distinguish between the lipoxygenase isoenzymes and to check the purity of enzyme preparations.

Original language	English
Pages (from-to)	281-285
Number of pages	5
Journal	Phytochemical Analysis
Volume	5
Issue number	6
DOIs	https://doi.org/10.1002/pca.2800050602
Publication status	Published - 1994
Externally published	Yes

Keywords

electrospray ionization
Isoenzymes
mass spectrometry
matrix assisted laser desorption/ionization
molecular mass determination
soybean lipoxygenase

Access to Document

10.1002/pca.2800050602

Cite this

@article{a5634cfb55aa4f938ba5e2779718a1a4,

title = "Accurate molecular mass determination by mass spectrometrical techniques: Differentiation of soybean lipoxygenase isoenzymes",

abstract = "Molecular mass determinations for the four lipoxygenase isoenzymes from soybean seeds were compared using two mass spectrometrical (MS) techniques as well as gel permeation chromatography and sodium dodecylsulphate‐polyacrylamide gel electrophoresis. The data obtained by MS techniques were in good agreement with the molecular masses evaluated from the corresponding cDNA. The MS techniques allowed to distinguish between the lipoxygenase isoenzymes and to check the purity of enzyme preparations.",

keywords = "electrospray ionization, Isoenzymes, mass spectrometry, matrix assisted laser desorption/ionization, molecular mass determination, soybean lipoxygenase",

author = "G. Scheller and B. Stahl and S. Weyd and M. Karas and F. Hillenkamp and P. Schreier",

year = "1994",

doi = "10.1002/pca.2800050602",

language = "English",

volume = "5",

pages = "281--285",

journal = "Phytochemical Analysis",

issn = "0958-0344",

publisher = "John Wiley and Sons Ltd",

number = "6",

}

TY - JOUR

T1 - Accurate molecular mass determination by mass spectrometrical techniques

T2 - Differentiation of soybean lipoxygenase isoenzymes

AU - Scheller, G.

AU - Stahl, B.

AU - Weyd, S.

AU - Karas, M.

AU - Hillenkamp, F.

AU - Schreier, P.

PY - 1994

Y1 - 1994

N2 - Molecular mass determinations for the four lipoxygenase isoenzymes from soybean seeds were compared using two mass spectrometrical (MS) techniques as well as gel permeation chromatography and sodium dodecylsulphate‐polyacrylamide gel electrophoresis. The data obtained by MS techniques were in good agreement with the molecular masses evaluated from the corresponding cDNA. The MS techniques allowed to distinguish between the lipoxygenase isoenzymes and to check the purity of enzyme preparations.

AB - Molecular mass determinations for the four lipoxygenase isoenzymes from soybean seeds were compared using two mass spectrometrical (MS) techniques as well as gel permeation chromatography and sodium dodecylsulphate‐polyacrylamide gel electrophoresis. The data obtained by MS techniques were in good agreement with the molecular masses evaluated from the corresponding cDNA. The MS techniques allowed to distinguish between the lipoxygenase isoenzymes and to check the purity of enzyme preparations.

KW - electrospray ionization

KW - Isoenzymes

KW - mass spectrometry

KW - matrix assisted laser desorption/ionization

KW - molecular mass determination

KW - soybean lipoxygenase

UR - http://www.scopus.com/inward/record.url?scp=0028191648&partnerID=8YFLogxK

U2 - 10.1002/pca.2800050602

DO - 10.1002/pca.2800050602

M3 - Article

AN - SCOPUS:0028191648

SN - 0958-0344

VL - 5

SP - 281

EP - 285

JO - Phytochemical Analysis

JF - Phytochemical Analysis

IS - 6

ER -

Accurate molecular mass determination by mass spectrometrical techniques: Differentiation of soybean lipoxygenase isoenzymes

Abstract

Keywords

Access to Document

Fingerprint

Cite this