Absorption spectroscopy of individual nano-objects and improved readout of DNA microarrays using photothermal detection

Stephane Berciaud*, Laurent Cognet, Gerhard A. Blab, David Lasne, José Remacle, Philippe Tamarat, Brahim Lounis

*Corresponding author for this work

Research output: Contribution to journalConference articleAcademicpeer-review

Abstract

We developed a photothermal method based on scattering around a nano-absorber that allows for the unprecedented detection of individual nano-objects such as gold nanoparticles with diameter down to 1.4 nm as well as CdSe nanocrystals. This method relies on the absorptive properties of the nano-object and does not suffer from the drawbacks of luminescence-based methods. We present here two different applications of this versatile detection method. First, we performed absorption spectroscopy of individual gold nanoparticles as small as 5nm and CdSe nanocrystals in the multiexcitonic regime. Second, we show the applicability of our method for new types of gold nanoparticles based DNA microarrays. In addition to the intrinsic signal stability due to the use of gold labelling, our technique does not require silver staining enhancement and permits to push the signal dynamics of such microarrays from the single nanoparticle detection to almost the full surface coverage.

Original languageEnglish
Article number60920A
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6092
DOIs
Publication statusPublished - 2006
EventUltrasensitive and Single-Molecule Detection Technologies - San Jose, CA, United States
Duration: 21 Jan 200624 Jan 2006

Keywords

  • Absorption spectroscopy
  • CdSe nanocrystals
  • DNA chips
  • Gold nanoparticles
  • Microscopy

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