A versatile genome-scale PCR-based pipeline for high-definition DNA FISH.

M. Bienko; N. Crosetto; L. Teytelman; S. Klemm; S. Itzkovitz; A. van Oudenaarden

A versatile genome-scale PCR-based pipeline for high-definition DNA FISH.

M. Bienko
, N. Crosetto
, L. Teytelman
, S. Klemm
, S. Itzkovitz
, A. van Oudenaarden

extern
University Medical Center Utrecht

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We developed a cost-effective genome-scale PCR-based method for high-definition DNA FISH (HD-FISH). We visualized gene loci with diffraction-limited resolution, chromosomes as spot clusters and single genes together with transcripts by combining HD-FISH with single-molecule RNA FISH. We provide a database of over 4.3 million primer pairs targeting the human and mouse genomes that is readily usable for rapid and flexible generation of probes.

Original language	Undefined/Unknown
Pages (from-to)	122-4
Number of pages	3
Journal	Nature Methods
Volume	10
Issue number	2
Publication status	Published - 2013
Externally published	Yes

Access to Document

http://www.ncbi.nlm.nih.gov/pubmed/23263692

Cite this

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title = "A versatile genome-scale PCR-based pipeline for high-definition DNA FISH.",

abstract = "We developed a cost-effective genome-scale PCR-based method for high-definition DNA FISH (HD-FISH). We visualized gene loci with diffraction-limited resolution, chromosomes as spot clusters and single genes together with transcripts by combining HD-FISH with single-molecule RNA FISH. We provide a database of over 4.3 million primer pairs targeting the human and mouse genomes that is readily usable for rapid and flexible generation of probes.",

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AU - Bienko, M.

AU - Crosetto, N.

AU - Teytelman, L.

AU - Klemm, S.

AU - Itzkovitz, S.

AU - van Oudenaarden, A.

PY - 2013

Y1 - 2013

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AB - We developed a cost-effective genome-scale PCR-based method for high-definition DNA FISH (HD-FISH). We visualized gene loci with diffraction-limited resolution, chromosomes as spot clusters and single genes together with transcripts by combining HD-FISH with single-molecule RNA FISH. We provide a database of over 4.3 million primer pairs targeting the human and mouse genomes that is readily usable for rapid and flexible generation of probes.

M3 - Article

SN - 1548-7091

VL - 10

SP - 122

EP - 124

JO - Nature Methods

JF - Nature Methods

IS - 2

ER -