A vaccine antigen central in influenza A(H5) virus antigenic space confers subtype-wide immunity

Adinda Kok, Samuel H Wilks, Sina Tureli, Sarah L James, Theo M Bestebroer, David F Burke, Mathis Funk, Stefan van der Vliet, Monique I Spronken, Willemijn F Rijnink, David Pattinson, Dennis de Meulder, Miruna E Rosu, Pascal Lexmond, Judith M A van den Brand, Sander Herfst, Derek J Smith, Ron A M Fouchier, Mathilde Richard*

*Corresponding author for this work

Research output: Working paperPreprintAcademic

Abstract

Highly pathogenic avian influenza A(H5) viruses globally impact wild and domestic birds, and mammals, including humans, underscoring their pandemic potential. The antigenic evolution of the A(H5) hemagglutinin (HA) poses challenges for pandemic preparedness and vaccine design. Here, the global antigenic evolution of the A(H5) HA was captured in a high-resolution antigenic map. The map was used to engineer immunogenic and antigenically central vaccine HA antigens, eliciting antibody responses that broadly cover the A(H5) antigenic space. In ferrets, a central antigen protected as well as homologous vaccines against heterologous infection with two antigenically distinct viruses. This work showcases the rational design of subtype-wide influenza A(H5) pre-pandemic vaccines and demonstrates the value of antigenic maps for the evaluation of vaccine-induced immune responses through antibody profiles.

Original languageEnglish
PublisherbioRxiv
Number of pages26
DOIs
Publication statusPublished - 6 Aug 2024

Publication series

NamebioRxiv
PublisherCold Spring Harbor Laboratory
ISSN (Print)2692-8205

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