A single amino acid substitution in the transmembrane envelope glycoprotein of feline immunodeficiency virus alters cellular tropism

T W Vahlenkamp; E J Verschoor; N N Schuurman; A L van Vliet; M C Horzinek; H F Egberink; A de Ronde

doi:10.1128/JVI.71.9.7132-7135.1997

A single amino acid substitution in the transmembrane envelope glycoprotein of feline immunodeficiency virus alters cellular tropism

T W Vahlenkamp, E J Verschoor, N N Schuurman, A L van Vliet, M C Horzinek, H F Egberink, A de Ronde

Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, The Netherlands. [email protected]

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The cellular tropism of the feline immunodeficiency virus (FIV) is affected by changes in variable region 3 (V3) of the surface (SU) envelope glycoprotein (Verschoor, E. J., et al., J. Virol. 69:4752-4757, 1995). By using high-dose DNA transfection, an FIV molecular clone with a non-CRFK-tropic V3 acquired the ability to replicate in CRFK cells. A single point mutation from a methionine to a threonine in the ectodomain of its transmembrane (TM) envelope glycoprotein was responsible for this change in viral tropism. This substitution is located in the putative SU interactive region, between the fusion peptide and the membrane-spanning region. Our results show that this region of the TM envelope glycoprotein constitutes an additional determinant for cell tropism.

Original language	English
Pages (from-to)	7132-5
Number of pages	4
Journal	Journal of Virology
Volume	71
Issue number	9
DOIs	https://doi.org/10.1128/JVI.71.9.7132-7135.1997
Publication status	Published - Sept 1997

Keywords

Amino Acid Sequence
Animals
Base Sequence
Cats
DNA
Glycoproteins/genetics
Immunodeficiency Virus, Feline/physiology
Methionine/metabolism
Molecular Sequence Data
Mutagenesis, Site-Directed
Viral Envelope Proteins/genetics

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10.1128/JVI.71.9.7132-7135.1997

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title = "A single amino acid substitution in the transmembrane envelope glycoprotein of feline immunodeficiency virus alters cellular tropism",

abstract = "The cellular tropism of the feline immunodeficiency virus (FIV) is affected by changes in variable region 3 (V3) of the surface (SU) envelope glycoprotein (Verschoor, E. J., et al., J. Virol. 69:4752-4757, 1995). By using high-dose DNA transfection, an FIV molecular clone with a non-CRFK-tropic V3 acquired the ability to replicate in CRFK cells. A single point mutation from a methionine to a threonine in the ectodomain of its transmembrane (TM) envelope glycoprotein was responsible for this change in viral tropism. This substitution is located in the putative SU interactive region, between the fusion peptide and the membrane-spanning region. Our results show that this region of the TM envelope glycoprotein constitutes an additional determinant for cell tropism.",

keywords = "Amino Acid Sequence, Animals, Base Sequence, Cats, DNA, Glycoproteins/genetics, Immunodeficiency Virus, Feline/physiology, Methionine/metabolism, Molecular Sequence Data, Mutagenesis, Site-Directed, Viral Envelope Proteins/genetics",

author = "Vahlenkamp, {T W} and Verschoor, {E J} and Schuurman, {N N} and {van Vliet}, {A L} and Horzinek, {M C} and Egberink, {H F} and {de Ronde}, A",

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T1 - A single amino acid substitution in the transmembrane envelope glycoprotein of feline immunodeficiency virus alters cellular tropism

AU - Vahlenkamp, T W

AU - Verschoor, E J

AU - Schuurman, N N

AU - van Vliet, A L

AU - Horzinek, M C

AU - Egberink, H F

AU - de Ronde, A

PY - 1997/9

Y1 - 1997/9

N2 - The cellular tropism of the feline immunodeficiency virus (FIV) is affected by changes in variable region 3 (V3) of the surface (SU) envelope glycoprotein (Verschoor, E. J., et al., J. Virol. 69:4752-4757, 1995). By using high-dose DNA transfection, an FIV molecular clone with a non-CRFK-tropic V3 acquired the ability to replicate in CRFK cells. A single point mutation from a methionine to a threonine in the ectodomain of its transmembrane (TM) envelope glycoprotein was responsible for this change in viral tropism. This substitution is located in the putative SU interactive region, between the fusion peptide and the membrane-spanning region. Our results show that this region of the TM envelope glycoprotein constitutes an additional determinant for cell tropism.

AB - The cellular tropism of the feline immunodeficiency virus (FIV) is affected by changes in variable region 3 (V3) of the surface (SU) envelope glycoprotein (Verschoor, E. J., et al., J. Virol. 69:4752-4757, 1995). By using high-dose DNA transfection, an FIV molecular clone with a non-CRFK-tropic V3 acquired the ability to replicate in CRFK cells. A single point mutation from a methionine to a threonine in the ectodomain of its transmembrane (TM) envelope glycoprotein was responsible for this change in viral tropism. This substitution is located in the putative SU interactive region, between the fusion peptide and the membrane-spanning region. Our results show that this region of the TM envelope glycoprotein constitutes an additional determinant for cell tropism.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - Cats

KW - DNA

KW - Glycoproteins/genetics

KW - Immunodeficiency Virus, Feline/physiology

KW - Methionine/metabolism

KW - Molecular Sequence Data

KW - Mutagenesis, Site-Directed

KW - Viral Envelope Proteins/genetics

U2 - 10.1128/JVI.71.9.7132-7135.1997

DO - 10.1128/JVI.71.9.7132-7135.1997

M3 - Article

C2 - 9261450

SN - 0022-538X

VL - 71

SP - 7132

EP - 7135

JO - Journal of Virology

JF - Journal of Virology

IS - 9

ER -

A single amino acid substitution in the transmembrane envelope glycoprotein of feline immunodeficiency virus alters cellular tropism

Abstract

Keywords

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