A simple and convenient method for the hydrolysis of styrene-maleic anhydride copolymers to styrene-maleic acid copolymers

Adrian H Kopf; Martijn C Koorengevel; Cornelis A van Walree; Timothy R Dafforn; J Antoinette Killian

doi:10.1016/j.chemphyslip.2018.11.011

A simple and convenient method for the hydrolysis of styrene-maleic anhydride copolymers to styrene-maleic acid copolymers

Adrian H Kopf, Martijn C Koorengevel, Cornelis A van Walree, Timothy R Dafforn, J Antoinette Killian

School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B152TT, United Kingdom.

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Styrene-maleic acid (SMA) copolymers are increasingly gaining attention in the membrane protein field due to their ability to solubilize lipid membranes into discoidal nanoparticles. The copolymers are synthesized as styrene-maleic anhydride (SMAnh), and need to be converted to the free acid form (SMA) before they are capable of solubilizing membranes. This hydrolysis reaction is traditionally performed under rather cumbersome reflux conditions. Here we report an alternative method for the hydrolysis reaction using simple and readily available equipment found in virtually all biochemical laboratories, namely an autoclave. Based on the results we propose an optimum set of standard conditions for the hydrolysis reaction, that should make the method easily accessible to a wide scope of researchers.

Original language	English
Pages (from-to)	85-90
Number of pages	6
Journal	Chemistry and Physics of Lipids
Volume	218
Early online date	1 Dec 2018
DOIs	https://doi.org/10.1016/j.chemphyslip.2018.11.011
Publication status	Published - Jan 2019

Keywords

SMAnh
SMA
Hydrolysis reaction
SMALPs
Native nanodiscs
Autoclave

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10.1016/j.chemphyslip.2018.11.011

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title = "A simple and convenient method for the hydrolysis of styrene-maleic anhydride copolymers to styrene-maleic acid copolymers",

abstract = "Styrene-maleic acid (SMA) copolymers are increasingly gaining attention in the membrane protein field due to their ability to solubilize lipid membranes into discoidal nanoparticles. The copolymers are synthesized as styrene-maleic anhydride (SMAnh), and need to be converted to the free acid form (SMA) before they are capable of solubilizing membranes. This hydrolysis reaction is traditionally performed under rather cumbersome reflux conditions. Here we report an alternative method for the hydrolysis reaction using simple and readily available equipment found in virtually all biochemical laboratories, namely an autoclave. Based on the results we propose an optimum set of standard conditions for the hydrolysis reaction, that should make the method easily accessible to a wide scope of researchers.",

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doi = "10.1016/j.chemphyslip.2018.11.011",

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T1 - A simple and convenient method for the hydrolysis of styrene-maleic anhydride copolymers to styrene-maleic acid copolymers

AU - Kopf, Adrian H

AU - Koorengevel, Martijn C

AU - van Walree, Cornelis A

AU - Dafforn, Timothy R

AU - Killian, J Antoinette

PY - 2019/1

Y1 - 2019/1

N2 - Styrene-maleic acid (SMA) copolymers are increasingly gaining attention in the membrane protein field due to their ability to solubilize lipid membranes into discoidal nanoparticles. The copolymers are synthesized as styrene-maleic anhydride (SMAnh), and need to be converted to the free acid form (SMA) before they are capable of solubilizing membranes. This hydrolysis reaction is traditionally performed under rather cumbersome reflux conditions. Here we report an alternative method for the hydrolysis reaction using simple and readily available equipment found in virtually all biochemical laboratories, namely an autoclave. Based on the results we propose an optimum set of standard conditions for the hydrolysis reaction, that should make the method easily accessible to a wide scope of researchers.

AB - Styrene-maleic acid (SMA) copolymers are increasingly gaining attention in the membrane protein field due to their ability to solubilize lipid membranes into discoidal nanoparticles. The copolymers are synthesized as styrene-maleic anhydride (SMAnh), and need to be converted to the free acid form (SMA) before they are capable of solubilizing membranes. This hydrolysis reaction is traditionally performed under rather cumbersome reflux conditions. Here we report an alternative method for the hydrolysis reaction using simple and readily available equipment found in virtually all biochemical laboratories, namely an autoclave. Based on the results we propose an optimum set of standard conditions for the hydrolysis reaction, that should make the method easily accessible to a wide scope of researchers.

KW - SMAnh

KW - SMA

KW - Hydrolysis reaction

KW - SMALPs

KW - Native nanodiscs

KW - Autoclave

U2 - 10.1016/j.chemphyslip.2018.11.011

DO - 10.1016/j.chemphyslip.2018.11.011

M3 - Article

C2 - 30513281

SN - 0009-3084

VL - 218

SP - 85

EP - 90

JO - Chemistry and Physics of Lipids

JF - Chemistry and Physics of Lipids

ER -

A simple and convenient method for the hydrolysis of styrene-maleic anhydride copolymers to styrene-maleic acid copolymers

Abstract

Keywords

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