A screening method for decoloration of xenobiotic dyes by fungi

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Abstract

Wood degrading fungi are often screened for their ability to degrade xenobiotics such as dyes. Dye decoloration by these fungi on solid media could until now only be assessed qualitatively. We here describe a fast quantitative method to screen for dye decoloration on such media. Decoloration of crystal violet (CV), malachite green (MG), orange G (OG), rose bengal (RB) and remazol brilliant blue R (RBBR) by 124 isolates of the basidiomycete Schizophyllum commune was quantified with a flatbed scanner and the CIE-L*a*b* model. Colour and intensity changes were calculated with the Euclidean distance formula. More than 10 strains showed high MG decoloration. Isolates 136, 140 and 213 showed superior CV decoloration, while OG was most effectively decolorized by isolates 183, 216 and 227. Six strains showed high RB decoloration with isolate 216 being superior. The latter strain was also highly active on RBBR together with isolates 177 and 227. Together, dye decoloration was highly variable between the 124 isolates but strain 216 showed high activity on 3 out of 5 dyes. The fast screening method described in this paper enables identification of strains effectively decolorizing dyes.

Original languageEnglish
Article number106301
Pages (from-to)1-4
JournalJournal of Microbiological Methods
Volume188
DOIs
Publication statusPublished - Sept 2021

Bibliographical note

Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Funding Information:
Part of this research was supported by Oasen that collaborates with Utrecht University on water purification technology.

Publisher Copyright:
© 2021 The Authors

Keywords

  • Basidiomycete
  • Bioremediation
  • Dye decoloration
  • Fungus
  • Schizophyllum commune
  • Xenobiotic degradation

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