Abstract
Rat/mouse T cell hybridoma-derived PC60 R55/R75 cells were used as a model to study the role of intracellular potassium in TNF-induced apoptosis and gene induction. A reduction of intracellular potassium with nigericin or valinomycin (ionophores), or ouabain (Na(+)/K(+)-ATPase inhibitor) sensitized PC60 R55/R75 cells to TNF-induced apoptosis. TNF-induced GM-CSF release in PC60 R55/R75 cells was enhanced by nigericin or ouabain. Similar results were obtained with human cervix carcinoma cells HeLaH21 exposed to TNF. These results suggest a role for intracellular potassium in TNF-induced apoptosis and gene induction.
| Original language | English |
|---|---|
| Pages (from-to) | 747-50 |
| Number of pages | 4 |
| Journal | Cytokine |
| Volume | 12 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 2000 |
Bibliographical note
Copyright 2000 Academic Press.Keywords
- Animals
- Apoptosis
- Cell Survival
- Gene Expression Regulation, Neoplastic
- Granulocyte-Macrophage Colony-Stimulating Factor
- HeLa Cells
- Humans
- Hybridomas
- Mice
- Nigericin
- Ouabain
- Potassium
- Recombinant Proteins
- T-Lymphocytes
- Transcriptional Activation
- Tumor Necrosis Factor-alpha
- Valinomycin
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