A role for potassium in TNF-induced apoptosis and gene-induction in human and rodent tumour cell lines

L C Penning; G Denecker; D Vercammen; W Declercq; R G Schipper; P Vandenabeele

doi:10.1006/cyto.1999.0626

A role for potassium in TNF-induced apoptosis and gene-induction in human and rodent tumour cell lines

L C Penning, G Denecker, D Vercammen, W Declercq, R G Schipper, P Vandenabeele

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Rat/mouse T cell hybridoma-derived PC60 R55/R75 cells were used as a model to study the role of intracellular potassium in TNF-induced apoptosis and gene induction. A reduction of intracellular potassium with nigericin or valinomycin (ionophores), or ouabain (Na(+)/K(+)-ATPase inhibitor) sensitized PC60 R55/R75 cells to TNF-induced apoptosis. TNF-induced GM-CSF release in PC60 R55/R75 cells was enhanced by nigericin or ouabain. Similar results were obtained with human cervix carcinoma cells HeLaH21 exposed to TNF. These results suggest a role for intracellular potassium in TNF-induced apoptosis and gene induction.

Original language	English
Pages (from-to)	747-50
Number of pages	4
Journal	Cytokine
Volume	12
Issue number	6
DOIs	https://doi.org/10.1006/cyto.1999.0626
Publication status	Published - Jun 2000

Keywords

Animals
Apoptosis
Cell Survival
Gene Expression Regulation, Neoplastic
Granulocyte-Macrophage Colony-Stimulating Factor
HeLa Cells
Humans
Hybridomas
Mice
Nigericin
Ouabain
Potassium
Recombinant Proteins
T-Lymphocytes
Transcriptional Activation
Tumor Necrosis Factor-alpha
Valinomycin

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10.1006/cyto.1999.0626

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title = "A role for potassium in TNF-induced apoptosis and gene-induction in human and rodent tumour cell lines",

abstract = "Rat/mouse T cell hybridoma-derived PC60 R55/R75 cells were used as a model to study the role of intracellular potassium in TNF-induced apoptosis and gene induction. A reduction of intracellular potassium with nigericin or valinomycin (ionophores), or ouabain (Na(+)/K(+)-ATPase inhibitor) sensitized PC60 R55/R75 cells to TNF-induced apoptosis. TNF-induced GM-CSF release in PC60 R55/R75 cells was enhanced by nigericin or ouabain. Similar results were obtained with human cervix carcinoma cells HeLaH21 exposed to TNF. These results suggest a role for intracellular potassium in TNF-induced apoptosis and gene induction.",

keywords = "Animals, Apoptosis, Cell Survival, Gene Expression Regulation, Neoplastic, Granulocyte-Macrophage Colony-Stimulating Factor, HeLa Cells, Humans, Hybridomas, Mice, Nigericin, Ouabain, Potassium, Recombinant Proteins, T-Lymphocytes, Transcriptional Activation, Tumor Necrosis Factor-alpha, Valinomycin",

author = "Penning, {L C} and G Denecker and D Vercammen and W Declercq and Schipper, {R G} and P Vandenabeele",

year = "2000",

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doi = "10.1006/cyto.1999.0626",

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AU - Penning, L C

AU - Denecker, G

AU - Vercammen, D

AU - Declercq, W

AU - Schipper, R G

AU - Vandenabeele, P

PY - 2000/6

Y1 - 2000/6

N2 - Rat/mouse T cell hybridoma-derived PC60 R55/R75 cells were used as a model to study the role of intracellular potassium in TNF-induced apoptosis and gene induction. A reduction of intracellular potassium with nigericin or valinomycin (ionophores), or ouabain (Na(+)/K(+)-ATPase inhibitor) sensitized PC60 R55/R75 cells to TNF-induced apoptosis. TNF-induced GM-CSF release in PC60 R55/R75 cells was enhanced by nigericin or ouabain. Similar results were obtained with human cervix carcinoma cells HeLaH21 exposed to TNF. These results suggest a role for intracellular potassium in TNF-induced apoptosis and gene induction.

AB - Rat/mouse T cell hybridoma-derived PC60 R55/R75 cells were used as a model to study the role of intracellular potassium in TNF-induced apoptosis and gene induction. A reduction of intracellular potassium with nigericin or valinomycin (ionophores), or ouabain (Na(+)/K(+)-ATPase inhibitor) sensitized PC60 R55/R75 cells to TNF-induced apoptosis. TNF-induced GM-CSF release in PC60 R55/R75 cells was enhanced by nigericin or ouabain. Similar results were obtained with human cervix carcinoma cells HeLaH21 exposed to TNF. These results suggest a role for intracellular potassium in TNF-induced apoptosis and gene induction.

KW - Animals

KW - Apoptosis

KW - Cell Survival

KW - Gene Expression Regulation, Neoplastic

KW - Granulocyte-Macrophage Colony-Stimulating Factor

KW - HeLa Cells

KW - Humans

KW - Hybridomas

KW - Mice

KW - Nigericin

KW - Ouabain

KW - Potassium

KW - Recombinant Proteins

KW - T-Lymphocytes

KW - Transcriptional Activation

KW - Tumor Necrosis Factor-alpha

KW - Valinomycin

U2 - 10.1006/cyto.1999.0626

DO - 10.1006/cyto.1999.0626

M3 - Article

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SN - 1043-4666

VL - 12

SP - 747

EP - 750

JO - Cytokine

JF - Cytokine

IS - 6

ER -

A role for potassium in TNF-induced apoptosis and gene-induction in human and rodent tumour cell lines

Abstract

Keywords

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