A particulate membrane preparation from ovaries of preovulatory rainbow trout (Salmo gairdnerii): Binding studies with 125I-human chorionic gonadotropin

Rüdiger Schulz; Reiner Schlaghecke; Volker Blüm

doi:10.1016/0300-9629(85)90879-5

A particulate membrane preparation from ovaries of preovulatory rainbow trout (Salmo gairdnerii): Binding studies with ¹²⁵I-human chorionic gonadotropin

Rüdiger Schulz^*, Reiner Schlaghecke, Volker Blüm

^*Corresponding author for this work

Environmental Biology

Heinrich Heine University Düsseldorf

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

1. 1. In order to obtain a particulate membrane preparation from complete ovaries of previtellogenic and vitellogenic trout, gonads were homogenized in a phosphate-buffered 0.5 M NaCl solution. 2. 2. Radio-receptor assay studies were carried out with these preparations using ¹²⁵I-hCG as the ligand and binding-sites showed high-affinity (K_A = 1.6-2.9 × 10⁹), low-capacity (15.3-149.7 pg hCG/mg freshweight) and target-organ specificity. 3. 3. We conclude that the method of membrane preparation is suitable for processing yolk-laden ovaries and that the binding sites can be assayed-with some restrictions-using ¹²⁵I-hCG as the ligand.

Original language	English
Pages (from-to)	429-433
Number of pages	5
Journal	Comparative Biochemistry and Physiology -- Part A: Physiology
Volume	82
Issue number	2
DOIs	https://doi.org/10.1016/0300-9629(85)90879-5
Publication status	Published - 1985

Bibliographical note

Funding Information:
Acknowledgements-This study was supportedb y DFG-grantB l 62/15-l to V. B&m. R. Schulzi s indebtedt o the NIADDK (Baltimore,U SA) for the gift of highly purified hCG and to R. Oberstebrink-Scholflo r expert technical assistance.

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

Funding

Acknowledgements-This study was supportedb y DFG-grantB l 62/15-l to V. B&m. R. Schulzi s indebtedt o the NIADDK (Baltimore,U SA) for the gift of highly purified hCG and to R. Oberstebrink-Scholflo r expert technical assistance.

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10.1016/0300-9629(85)90879-5

Cite this

@article{10a5e0c176c14dc4ae7a2cb23935e864,

title = "A particulate membrane preparation from ovaries of preovulatory rainbow trout (Salmo gairdnerii): Binding studies with 125I-human chorionic gonadotropin",

abstract = "1. 1. In order to obtain a particulate membrane preparation from complete ovaries of previtellogenic and vitellogenic trout, gonads were homogenized in a phosphate-buffered 0.5 M NaCl solution. 2. 2. Radio-receptor assay studies were carried out with these preparations using 125I-hCG as the ligand and binding-sites showed high-affinity (KA = 1.6-2.9 × 109), low-capacity (15.3-149.7 pg hCG/mg freshweight) and target-organ specificity. 3. 3. We conclude that the method of membrane preparation is suitable for processing yolk-laden ovaries and that the binding sites can be assayed-with some restrictions-using 125I-hCG as the ligand.",

author = "R{\"u}diger Schulz and Reiner Schlaghecke and Volker Bl{\"u}m",

note = "Funding Information: Acknowledgements-This study was supportedb y DFG-grantB l 62/15-l to V. B&m. R. Schulzi s indebtedt o the NIADDK (Baltimore,U SA) for the gift of highly purified hCG and to R. Oberstebrink-Scholflo r expert technical assistance. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1985",

doi = "10.1016/0300-9629(85)90879-5",

language = "English",

volume = "82",

pages = "429--433",

journal = "Comparative Biochemistry and Physiology -- Part A: Physiology",

issn = "0300-9629",

publisher = "Elsevier Inc.",

number = "2",

}

A particulate membrane preparation from ovaries of preovulatory rainbow trout (Salmo gairdnerii): Binding studies with ¹²⁵I-human chorionic gonadotropin. / Schulz, Rüdiger; Schlaghecke, Reiner; Blüm, Volker.
In: Comparative Biochemistry and Physiology -- Part A: Physiology, Vol. 82, No. 2, 1985, p. 429-433.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - A particulate membrane preparation from ovaries of preovulatory rainbow trout (Salmo gairdnerii)

T2 - Binding studies with 125I-human chorionic gonadotropin

AU - Schulz, Rüdiger

AU - Schlaghecke, Reiner

AU - Blüm, Volker

N1 - Funding Information: Acknowledgements-This study was supportedb y DFG-grantB l 62/15-l to V. B&m. R. Schulzi s indebtedt o the NIADDK (Baltimore,U SA) for the gift of highly purified hCG and to R. Oberstebrink-Scholflo r expert technical assistance. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1985

Y1 - 1985

N2 - 1. 1. In order to obtain a particulate membrane preparation from complete ovaries of previtellogenic and vitellogenic trout, gonads were homogenized in a phosphate-buffered 0.5 M NaCl solution. 2. 2. Radio-receptor assay studies were carried out with these preparations using 125I-hCG as the ligand and binding-sites showed high-affinity (KA = 1.6-2.9 × 109), low-capacity (15.3-149.7 pg hCG/mg freshweight) and target-organ specificity. 3. 3. We conclude that the method of membrane preparation is suitable for processing yolk-laden ovaries and that the binding sites can be assayed-with some restrictions-using 125I-hCG as the ligand.

AB - 1. 1. In order to obtain a particulate membrane preparation from complete ovaries of previtellogenic and vitellogenic trout, gonads were homogenized in a phosphate-buffered 0.5 M NaCl solution. 2. 2. Radio-receptor assay studies were carried out with these preparations using 125I-hCG as the ligand and binding-sites showed high-affinity (KA = 1.6-2.9 × 109), low-capacity (15.3-149.7 pg hCG/mg freshweight) and target-organ specificity. 3. 3. We conclude that the method of membrane preparation is suitable for processing yolk-laden ovaries and that the binding sites can be assayed-with some restrictions-using 125I-hCG as the ligand.

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