TY - JOUR
T1 - A particulate membrane preparation from ovaries of preovulatory rainbow trout (Salmo gairdnerii)
T2 - Binding studies with 125I-human chorionic gonadotropin
AU - Schulz, Rüdiger
AU - Schlaghecke, Reiner
AU - Blüm, Volker
N1 - Funding Information:
Acknowledgements-This study was supportedb y DFG-grantB l 62/15-l to V. B&m. R. Schulzi s indebtedt o the NIADDK (Baltimore,U SA) for the gift of highly purified hCG and to R. Oberstebrink-Scholflo r expert technical assistance.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1985
Y1 - 1985
N2 - 1. 1. In order to obtain a particulate membrane preparation from complete ovaries of previtellogenic and vitellogenic trout, gonads were homogenized in a phosphate-buffered 0.5 M NaCl solution. 2. 2. Radio-receptor assay studies were carried out with these preparations using 125I-hCG as the ligand and binding-sites showed high-affinity (KA = 1.6-2.9 × 109), low-capacity (15.3-149.7 pg hCG/mg freshweight) and target-organ specificity. 3. 3. We conclude that the method of membrane preparation is suitable for processing yolk-laden ovaries and that the binding sites can be assayed-with some restrictions-using 125I-hCG as the ligand.
AB - 1. 1. In order to obtain a particulate membrane preparation from complete ovaries of previtellogenic and vitellogenic trout, gonads were homogenized in a phosphate-buffered 0.5 M NaCl solution. 2. 2. Radio-receptor assay studies were carried out with these preparations using 125I-hCG as the ligand and binding-sites showed high-affinity (KA = 1.6-2.9 × 109), low-capacity (15.3-149.7 pg hCG/mg freshweight) and target-organ specificity. 3. 3. We conclude that the method of membrane preparation is suitable for processing yolk-laden ovaries and that the binding sites can be assayed-with some restrictions-using 125I-hCG as the ligand.
UR - http://www.scopus.com/inward/record.url?scp=0021970634&partnerID=8YFLogxK
U2 - 10.1016/0300-9629(85)90879-5
DO - 10.1016/0300-9629(85)90879-5
M3 - Article
AN - SCOPUS:0021970634
SN - 0300-9629
VL - 82
SP - 429
EP - 433
JO - Comparative Biochemistry and Physiology -- Part A: Physiology
JF - Comparative Biochemistry and Physiology -- Part A: Physiology
IS - 2
ER -