A novel heterocyst glycolipid detected in a pelagic N2-fixing cyanobacterium of the genus Calothrix

Nicole J. Bale; Ellen C. Hopmans; Denise Dorhout; Lucas J. Stal; Michele Grego; Judith van Bleijswijk; Jaap S. Sinninghe Damsté; Stefan Schouten

doi:10.1016/j.orggeochem.2018.06.009

A novel heterocyst glycolipid detected in a pelagic N₂-fixing cyanobacterium of the genus Calothrix

Nicole J. Bale^*
, Ellen C. Hopmans
, Denise Dorhout
, Lucas J. Stal
, Michele Grego
, Judith van Bleijswijk
, Jaap S. Sinninghe Damsté
, Stefan Schouten

^*Corresponding author for this work

Royal Netherlands Institute for Sea Research - NIOZ

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Previous studies have shown that heterocyst glycolipids (HGs) are unique markers for N₂-fixing heterocystous cyanobacteria. In this study, the HGs of a marine pelagic Calothrix sp. CCY1611 isolated from the tropical western North Atlantic were analyzed by ultra-high pressure liquid chromatography–high resolution mass spectrometry and it was shown that this organism contains an unusual C₂₈ triol HG with a methylated C₆ sugar (methyl-HG₂₈ triol) head group. Gas chromatography–mass spectrometry analysis of the sugar released from the novel HG by acid methanolysis revealed that the sugar is likely 6-O-methyl-β-D-glucopyranose. We propose that this methyl-HG₂₈ triol is a potential biomarker for pelagic members of the genus Calothrix.

Original language	English
Pages (from-to)	44-47
Number of pages	4
Journal	Organic Geochemistry
Volume	123
DOIs	https://doi.org/10.1016/j.orggeochem.2018.06.009
Publication status	Published - 1 Sept 2018

Funding

We would like to thank John Volkman and Bart van Dongen for their helpful comments. We thank J. Ossebaar, A. Mets and S. Vreugdenhil for analytical and technical support. We thank Professor T. Villareal for helpful discussions on this subject. This work was supported by the Netherlands Organisation for Scientific Research (NWO) through grant 822.01.017 to S.S. This project has also received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No 694569 to J.S.S.D). S.S. and J.S.S.D were financially supported by the Netherlands Earth Systems Science Centre (NESSC) , a Gravitation grant ( 024.002.001 ) from the Dutch Ministry of Education, Culture and Science.

Funders	Funder number
Nederlandse Organisatie voor Wetenschappelijke Onderzoek (NWO)	822.01.017
European Research Council (ERC) under the European Union	694569
Netherlands Earth Systems Science Centre (NESSC), from the Dutch Ministry of Education, Culture and Science	024.002.001
European Research Council	694569

Keywords

6-O-methyl-β-D-glucopyranose
Calothrix
Heterocyst glycolipids
Heterocystous cyanobacteria
N-fixation
Pelagic

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.orggeochem.2018.06.009

A novelFinal published version, 276 KBLicence: Taverne

Cite this

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title = "A novel heterocyst glycolipid detected in a pelagic N2-fixing cyanobacterium of the genus Calothrix",

abstract = "Previous studies have shown that heterocyst glycolipids (HGs) are unique markers for N2-fixing heterocystous cyanobacteria. In this study, the HGs of a marine pelagic Calothrix sp. CCY1611 isolated from the tropical western North Atlantic were analyzed by ultra-high pressure liquid chromatography–high resolution mass spectrometry and it was shown that this organism contains an unusual C28 triol HG with a methylated C6 sugar (methyl-HG28 triol) head group. Gas chromatography–mass spectrometry analysis of the sugar released from the novel HG by acid methanolysis revealed that the sugar is likely 6-O-methyl-β-D-glucopyranose. We propose that this methyl-HG28 triol is a potential biomarker for pelagic members of the genus Calothrix.",

keywords = "6-O-methyl-β-D-glucopyranose, Calothrix, Heterocyst glycolipids, Heterocystous cyanobacteria, N-fixation, Pelagic",

author = "Bale, \{Nicole J.\} and Hopmans, \{Ellen C.\} and Denise Dorhout and Stal, \{Lucas J.\} and Michele Grego and \{van Bleijswijk\}, Judith and \{Sinninghe Damst{\'e}\}, \{Jaap S.\} and Stefan Schouten",

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doi = "10.1016/j.orggeochem.2018.06.009",

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T1 - A novel heterocyst glycolipid detected in a pelagic N2-fixing cyanobacterium of the genus Calothrix

AU - Bale, Nicole J.

AU - Hopmans, Ellen C.

AU - Dorhout, Denise

AU - Stal, Lucas J.

AU - Grego, Michele

AU - van Bleijswijk, Judith

AU - Sinninghe Damsté, Jaap S.

AU - Schouten, Stefan

PY - 2018/9/1

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AB - Previous studies have shown that heterocyst glycolipids (HGs) are unique markers for N2-fixing heterocystous cyanobacteria. In this study, the HGs of a marine pelagic Calothrix sp. CCY1611 isolated from the tropical western North Atlantic were analyzed by ultra-high pressure liquid chromatography–high resolution mass spectrometry and it was shown that this organism contains an unusual C28 triol HG with a methylated C6 sugar (methyl-HG28 triol) head group. Gas chromatography–mass spectrometry analysis of the sugar released from the novel HG by acid methanolysis revealed that the sugar is likely 6-O-methyl-β-D-glucopyranose. We propose that this methyl-HG28 triol is a potential biomarker for pelagic members of the genus Calothrix.

KW - 6-O-methyl-β-D-glucopyranose

KW - Calothrix

KW - Heterocyst glycolipids

KW - Heterocystous cyanobacteria

KW - N-fixation

KW - Pelagic

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