A novel cetacean adenovirus in stranded harbour porpoises from the North Sea: detection and molecular characterization

Steven J van Beurden; Lonneke L IJsseldijk; Marco W G van de Bildt; Lineke Begeman; James F X Wellehan; Thomas B Waltzek; Geert de Vrieze; Andrea Gröne; Thijs Kuiken; M Hélène Verheije; Judit J Penzes

doi:10.1007/s00705-017-3310-8

A novel cetacean adenovirus in stranded harbour porpoises from the North Sea: detection and molecular characterization

Steven J van Beurden, Lonneke L IJsseldijk, Marco W G van de Bildt, Lineke Begeman, James F X Wellehan, Thomas B Waltzek, Geert de Vrieze, Andrea Gröne, Thijs Kuiken, M Hélène Verheije^*, Judit J Penzes

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Harbour porpoises (Phocoena phocoena) are the most prevalent cetaceans in the North Sea. The fecal viral flora of 21 harbour porpoises stranded along the Dutch coastline was analyzed by a metagenomics approach. Sequences of a novel cetacean mastadenovirus, designated harbour porpoise adenovirus 1 (HpAdV-1), were detected. The sequence of a 23-kbp genomic region, spanning the conserved late region, was determined using primer walking. Phylogenetic analysis indicated that HpAdV-1 is most closely related to bottlenose dolphin adenovirus and clusters with Cetartiodactyla adenoviruses. The prevalence of HpAdV-1 was low (2.6%) based on targeted PCR-screening of the intestinal contents of 151 harbour porpoises stranded between 2010 and 2013.

Original language	English
Pages (from-to)	2035–2040
Journal	Archives of Virology
Volume	162
Issue number	7
DOIs	https://doi.org/10.1007/s00705-017-3310-8
Publication status	Published - 2017

Keywords

Intestinal Conten
Bottlenose Dolphin
Harbour Porpoise
Protease Cleavage Site
Bowhead Whale

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10.1007/s00705-017-3310-8

10.1007%2Fs00705-017-3310-8Final published version, 507 KBLicence: Taverne

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van Beurden, S. J., IJsseldijk, L. L., van de Bildt, M. W. G., Begeman, L., Wellehan, J. F. X., Waltzek, T. B., de Vrieze, G., Gröne, A., Kuiken, T., Verheije, M. H., & Penzes, J. J. (2017). A novel cetacean adenovirus in stranded harbour porpoises from the North Sea: detection and molecular characterization. Archives of Virology, 162(7), 2035–2040. https://doi.org/10.1007/s00705-017-3310-8

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title = "A novel cetacean adenovirus in stranded harbour porpoises from the North Sea: detection and molecular characterization",

abstract = "Harbour porpoises (Phocoena phocoena) are the most prevalent cetaceans in the North Sea. The fecal viral flora of 21 harbour porpoises stranded along the Dutch coastline was analyzed by a metagenomics approach. Sequences of a novel cetacean mastadenovirus, designated harbour porpoise adenovirus 1 (HpAdV-1), were detected. The sequence of a 23-kbp genomic region, spanning the conserved late region, was determined using primer walking. Phylogenetic analysis indicated that HpAdV-1 is most closely related to bottlenose dolphin adenovirus and clusters with Cetartiodactyla adenoviruses. The prevalence of HpAdV-1 was low (2.6\%) based on targeted PCR-screening of the intestinal contents of 151 harbour porpoises stranded between 2010 and 2013.",

keywords = "Intestinal Conten, Bottlenose Dolphin , Harbour Porpoise , Protease Cleavage Site , Bowhead Whale",

author = "\{van Beurden\}, \{Steven J\} and IJsseldijk, \{Lonneke L\} and \{van de Bildt\}, \{Marco W G\} and Lineke Begeman and Wellehan, \{James F X\} and Waltzek, \{Thomas B\} and \{de Vrieze\}, Geert and Andrea Gr{\"o}ne and Thijs Kuiken and Verheije, \{M H{\'e}l{\`e}ne\} and Penzes, \{Judit J\}",

year = "2017",

doi = "10.1007/s00705-017-3310-8",

language = "English",

volume = "162",

pages = "2035–2040",

journal = "Archives of Virology",

issn = "0304-8608",

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van Beurden, SJ, IJsseldijk, LL, van de Bildt, MWG, Begeman, L, Wellehan, JFX, Waltzek, TB, de Vrieze, G, Gröne, A, Kuiken, T, Verheije, MH & Penzes, JJ 2017, 'A novel cetacean adenovirus in stranded harbour porpoises from the North Sea: detection and molecular characterization', Archives of Virology, vol. 162, no. 7, pp. 2035–2040. https://doi.org/10.1007/s00705-017-3310-8

TY - JOUR

T1 - A novel cetacean adenovirus in stranded harbour porpoises from the North Sea

T2 - detection and molecular characterization

AU - van Beurden, Steven J

AU - IJsseldijk, Lonneke L

AU - van de Bildt, Marco W G

AU - Begeman, Lineke

AU - Wellehan, James F X

AU - Waltzek, Thomas B

AU - de Vrieze, Geert

AU - Gröne, Andrea

AU - Kuiken, Thijs

AU - Verheije, M Hélène

AU - Penzes, Judit J

PY - 2017

Y1 - 2017

N2 - Harbour porpoises (Phocoena phocoena) are the most prevalent cetaceans in the North Sea. The fecal viral flora of 21 harbour porpoises stranded along the Dutch coastline was analyzed by a metagenomics approach. Sequences of a novel cetacean mastadenovirus, designated harbour porpoise adenovirus 1 (HpAdV-1), were detected. The sequence of a 23-kbp genomic region, spanning the conserved late region, was determined using primer walking. Phylogenetic analysis indicated that HpAdV-1 is most closely related to bottlenose dolphin adenovirus and clusters with Cetartiodactyla adenoviruses. The prevalence of HpAdV-1 was low (2.6%) based on targeted PCR-screening of the intestinal contents of 151 harbour porpoises stranded between 2010 and 2013.

AB - Harbour porpoises (Phocoena phocoena) are the most prevalent cetaceans in the North Sea. The fecal viral flora of 21 harbour porpoises stranded along the Dutch coastline was analyzed by a metagenomics approach. Sequences of a novel cetacean mastadenovirus, designated harbour porpoise adenovirus 1 (HpAdV-1), were detected. The sequence of a 23-kbp genomic region, spanning the conserved late region, was determined using primer walking. Phylogenetic analysis indicated that HpAdV-1 is most closely related to bottlenose dolphin adenovirus and clusters with Cetartiodactyla adenoviruses. The prevalence of HpAdV-1 was low (2.6%) based on targeted PCR-screening of the intestinal contents of 151 harbour porpoises stranded between 2010 and 2013.

KW - Intestinal Conten

KW - Bottlenose Dolphin

KW - Harbour Porpoise

KW - Protease Cleavage Site

KW - Bowhead Whale

U2 - 10.1007/s00705-017-3310-8

DO - 10.1007/s00705-017-3310-8

M3 - Article

C2 - 28283815

SN - 0304-8608

VL - 162

SP - 2035

EP - 2040

JO - Archives of Virology

JF - Archives of Virology

IS - 7

ER -

A novel cetacean adenovirus in stranded harbour porpoises from the North Sea: detection and molecular characterization

Abstract

Keywords

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