A monoclonal antibody that neutralizes poliovirus by cross-linking virions

A A Thomas, P Brioen, A Boeyé

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The neutralization of type 1 poliovirus by monoclonal antibody 35-1f4 was studied. The virions were rapidly linked by antibody into oligomers and larger aggregates, followed by slow redistribution of antibody between the immune complexes. The antibody content and infectivity of immune complexes were determined. Remaining single virions were fully infectious and free of antibody. The oligomers and larger aggregates did not significantly contribute to the residual infectivity, which therefore correlated with the number of remaining single virions. Papain digestion of neutralized poliovirus released fully infectious, antibody-free virions from the immune complexes. Anti-immunoglobulin antibodies reneutralized these virions. Polymerization was shown to occur even at virus concentrations of less than 10(3) PFU per ml.

Original languageEnglish
Pages (from-to)7-13
Number of pages7
JournalJournal of Virology
Volume54
Issue number1
Publication statusPublished - Apr 1985

Keywords

  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Antigen-Antibody Complex
  • Macromolecular Substances
  • Papain
  • Poliovirus

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