A Map of the Interactome Network of the Metazoan C. elegans

Siming Li; Christopher M. Armstrong; Nicolas Bertin; Hui Ge; Stuart Milstein; Mike Boxem; Pierre-Olivier Vidalain; Jing-Dong J. Han; Alban Chesneau; Tong Hao; Debra S. Goldberg; Ning Li; Monica Martinez; Jean-François Rual; Philippe Lamesch; Lai Xu; Muneesh Tewari; Sharyl L. Wong; Lan V. Zhang; Gabriel F. Berriz; Laurent Jacotot; Philippe Vaglio; Jérôme Reboul; Tomoko Hirozane-Kishikawa; Qianru Li; Harrison W. Gabel; Ahmed Elewa; Bridget Baumgartner; Debra J. Rose; Haiyuan Yu; Stephanie Bosak; Reynaldo Sequerra; Andrew Fraser; Susan E. Mango; William M. Saxton; Susan Strome; Sander van den Heuvel; Fabio Piano; Jean Vandenhaute; Claude Sardet; Mark Gerstein; Lynn Doucette-Stamm; Kristin C. Gunsalus; J. Wade Harper; Michael E. Cusick; Frederick P. Roth; David E. Hill; Marc Vidal

doi:10.1126/science.1091403

Laurent Jacotot, Philippe Vaglio, Jérôme Reboul, Tomoko Hirozane-Kishikawa, Qianru Li, Harrison W. Gabel, Ahmed Elewa, Bridget Baumgartner, Debra J. Rose, Haiyuan Yu, Stephanie Bosak, Reynaldo Sequerra, Andrew Fraser, Susan E. Mango, William M. Saxton, Susan Strome, Sander van den Heuvel, Fabio Piano, Jean Vandenhaute, Claude Sardet, Mark Gerstein, Lynn Doucette-Stamm, Kristin C. Gunsalus, J. Wade Harper, Michael E. Cusick, Frederick P. Roth, David E. Hill, Marc Vidal

Dana-Farber Cancer Institute and Department of Genetics, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA.
Institut de Génétique Moléculaire, Centre National de la Recherche Scientifique UMR 5535, 1919 Route de Mende, 34293 Montpellier Cedex 5, France.
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA.
Unité de Recherche en Biologie Moléculaire, Facultés Notre-Dame de la Paix, 61 Rue de Bruxelles, 5000 Namur, Belgium.
Verna and Marrs Department of Biochemistry and Molecular Biology, Program in Cell and Molecular Biology, Biophysics, and Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
Department of Biology, Indiana University, Jordan Hall 142, 1001 East Third Street, Bloomington, IN 47405, USA.
Department of Molecular Biophysics and Biochemistry and Department of Computer Science, Yale University, 266 Whitney Avenue, New Haven, CT 06520, USA.
Agencourt Bioscience Corporation, 100 Cummings Center, Suite 107G, Beverly, MA 01915, USA.
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK.
Huntsman Cancer Institute, University of Utah, 2000 Circle of Hope, Salt Lake City, UT 84112, USA.
Department of Biology, New York University, 1009 Silver Building, 100 Washington Square East, New York, NY 10003, USA.
Massachusetts General Hospital Cancer Center, Building 149, 13th Street, Charlestown, MA 02129, USA.

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

To initiate studies on how protein-protein interaction (or ``interactome'') networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains ~5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.

Original language	English
Pages (from-to)	540-544
Journal	Science
Volume	303
Issue number	5657
DOIs	https://doi.org/10.1126/science.1091403
Publication status	Published - 1 Jan 2004

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10.1126/science.1091403

http://adsabs.harvard.edu/abs/2004Sci...303..540L

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Li, S., Armstrong, C. M., Bertin, N., Ge, H., Milstein, S., Boxem, M., Vidalain, P.-O., Han, J.-D. J., Chesneau, A., Hao, T., Goldberg, D. S., Li, N., Martinez, M., Rual, J.-F., Lamesch, P., Xu, L., Tewari, M., Wong, S. L., Zhang, L. V., ... Vidal, M. (2004). A Map of the Interactome Network of the Metazoan C. elegans. Science, 303(5657), 540-544. https://doi.org/10.1126/science.1091403

@article{6816b93a7249467bb31a1fb67bef4571,

title = "A Map of the Interactome Network of the Metazoan C. elegans",

abstract = "To initiate studies on how protein-protein interaction (or ``interactome'') networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains \textasciitilde{}5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.",

author = "Siming Li and Armstrong, \{Christopher M.\} and Nicolas Bertin and Hui Ge and Stuart Milstein and Mike Boxem and Pierre-Olivier Vidalain and Han, \{Jing-Dong J.\} and Alban Chesneau and Tong Hao and Goldberg, \{Debra S.\} and Ning Li and Monica Martinez and Jean-Fran{\c c}ois Rual and Philippe Lamesch and Lai Xu and Muneesh Tewari and Wong, \{Sharyl L.\} and Zhang, \{Lan V.\} and Berriz, \{Gabriel F.\} and Laurent Jacotot and Philippe Vaglio and J{\'e}r{\^o}me Reboul and Tomoko Hirozane-Kishikawa and Qianru Li and Gabel, \{Harrison W.\} and Ahmed Elewa and Bridget Baumgartner and Rose, \{Debra J.\} and Haiyuan Yu and Stephanie Bosak and Reynaldo Sequerra and Andrew Fraser and Mango, \{Susan E.\} and Saxton, \{William M.\} and Susan Strome and \{van den Heuvel\}, Sander and Fabio Piano and Jean Vandenhaute and Claude Sardet and Mark Gerstein and Lynn Doucette-Stamm and Gunsalus, \{Kristin C.\} and Harper, \{J. Wade\} and Cusick, \{Michael E.\} and Roth, \{Frederick P.\} and Hill, \{David E.\} and Marc Vidal",

year = "2004",

month = jan,

day = "1",

doi = "10.1126/science.1091403",

language = "English",

volume = "303",

pages = "540--544",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "5657",

}

Li, S, Armstrong, CM, Bertin, N, Ge, H, Milstein, S, Boxem, M, Vidalain, P-O, Han, J-DJ, Chesneau, A, Hao, T, Goldberg, DS, Li, N, Martinez, M, Rual, J-F, Lamesch, P, Xu, L, Tewari, M, Wong, SL, Zhang, LV, Berriz, GF, Jacotot, L, Vaglio, P, Reboul, J, Hirozane-Kishikawa, T, Li, Q, Gabel, HW, Elewa, A, Baumgartner, B, Rose, DJ, Yu, H, Bosak, S, Sequerra, R, Fraser, A, Mango, SE, Saxton, WM, Strome, S, van den Heuvel, S, Piano, F, Vandenhaute, J, Sardet, C, Gerstein, M, Doucette-Stamm, L, Gunsalus, KC, Harper, JW, Cusick, ME, Roth, FP, Hill, DE & Vidal, M 2004, 'A Map of the Interactome Network of the Metazoan C. elegans', Science, vol. 303, no. 5657, pp. 540-544. https://doi.org/10.1126/science.1091403

TY - JOUR

T1 - A Map of the Interactome Network of the Metazoan C. elegans

AU - Li, Siming

AU - Armstrong, Christopher M.

AU - Bertin, Nicolas

AU - Ge, Hui

AU - Milstein, Stuart

AU - Boxem, Mike

AU - Vidalain, Pierre-Olivier

AU - Han, Jing-Dong J.

AU - Chesneau, Alban

AU - Hao, Tong

AU - Goldberg, Debra S.

AU - Li, Ning

AU - Martinez, Monica

AU - Rual, Jean-François

AU - Lamesch, Philippe

AU - Xu, Lai

AU - Tewari, Muneesh

AU - Wong, Sharyl L.

AU - Zhang, Lan V.

AU - Berriz, Gabriel F.

AU - Jacotot, Laurent

AU - Vaglio, Philippe

AU - Reboul, Jérôme

AU - Hirozane-Kishikawa, Tomoko

AU - Li, Qianru

AU - Gabel, Harrison W.

AU - Elewa, Ahmed

AU - Baumgartner, Bridget

AU - Rose, Debra J.

AU - Yu, Haiyuan

AU - Bosak, Stephanie

AU - Sequerra, Reynaldo

AU - Fraser, Andrew

AU - Mango, Susan E.

AU - Saxton, William M.

AU - Strome, Susan

AU - van den Heuvel, Sander

AU - Piano, Fabio

AU - Vandenhaute, Jean

AU - Sardet, Claude

AU - Gerstein, Mark

AU - Doucette-Stamm, Lynn

AU - Gunsalus, Kristin C.

AU - Harper, J. Wade

AU - Cusick, Michael E.

AU - Roth, Frederick P.

AU - Hill, David E.

AU - Vidal, Marc

PY - 2004/1/1

Y1 - 2004/1/1

N2 - To initiate studies on how protein-protein interaction (or ``interactome'') networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains ~5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.

AB - To initiate studies on how protein-protein interaction (or ``interactome'') networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains ~5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.

U2 - 10.1126/science.1091403

DO - 10.1126/science.1091403

M3 - Article

SN - 0036-8075

VL - 303

SP - 540

EP - 544

JO - Science

JF - Science

IS - 5657

ER -

A Map of the Interactome Network of the Metazoan C. elegans

Abstract

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