A Guide to Quantify Arabidopsis Seedling Thermomorphogenesis at Single Timepoints and by Interval Monitoring

Philipp Janitza; Zihao Zhu; Muhammad Usman Anwer; Martijn van Zanten; Carolin Delker

doi:10.1007/978-1-0716-3814-9_1

A Guide to Quantify Arabidopsis Seedling Thermomorphogenesis at Single Timepoints and by Interval Monitoring

Philipp Janitza, Zihao Zhu, Muhammad Usman Anwer, Martijn van Zanten, Carolin Delker^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

Abstract

Temperature-induced elongation of hypocotyls, petioles, and rootsRoots, together with hyponastic leaf responses, constitute key model phenotypes that can be used to assess a plant’s capacity for thermomorphogenesis. Phenotypic responses are often quantified at a single time point during seedling development at different temperatures. However, to capture growth dynamics, several time points need to be assessed, and ideally continuous measurements are taken. Here we describe a general experimental setup and technical solutions for recording and measuring seedling phenotypes at single and multiple time points. Furthermore, we present an R-package called “rootdetectR,” which allows easy processing of hypocotyl, rootRoot or petiole length, and growth rate data and provides different options of data presentation.

Original language	English
Title of host publication	Thermomorphogenesis
Publisher	Humana Press
Pages	3-16
Number of pages	14
Volume	2795
ISBN (Electronic)	978-1-0716-3814-9
ISBN (Print)	978-1-0716-3813-2
DOIs	https://doi.org/10.1007/978-1-0716-3814-9_1
Publication status	Published - 10 Apr 2024

Publication series

Name	Methods in Molecular Biology
Volume	2795
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.

Keywords

Growth rate analyses
Phenotyping
RootDetection
RootdetectR
Thermomorphogenesis

Access to Document

10.1007/978-1-0716-3814-9_1

978-1-0716-3814-9_1Final published version, 700 KBLicence: Taverne

Cite this

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title = "A Guide to Quantify Arabidopsis Seedling Thermomorphogenesis at Single Timepoints and by Interval Monitoring",

abstract = "Temperature-induced elongation of hypocotyls, petioles, and rootsRoots, together with hyponastic leaf responses, constitute key model phenotypes that can be used to assess a plant{\textquoteright}s capacity for thermomorphogenesis. Phenotypic responses are often quantified at a single time point during seedling development at different temperatures. However, to capture growth dynamics, several time points need to be assessed, and ideally continuous measurements are taken. Here we describe a general experimental setup and technical solutions for recording and measuring seedling phenotypes at single and multiple time points. Furthermore, we present an R-package called “rootdetectR,” which allows easy processing of hypocotyl, rootRoot or petiole length, and growth rate data and provides different options of data presentation.",

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A Guide to Quantify Arabidopsis Seedling Thermomorphogenesis at Single Timepoints and by Interval Monitoring. / Janitza, Philipp; Zhu, Zihao; Anwer, Muhammad Usman et al.
Thermomorphogenesis. Vol. 2795 Humana Press, 2024. p. 3-16 (Methods in Molecular Biology; Vol. 2795).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

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AU - van Zanten, Martijn

AU - Delker, Carolin

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A Guide to Quantify Arabidopsis Seedling Thermomorphogenesis at Single Timepoints and by Interval Monitoring

Abstract

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Keywords

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