A cool hybrid approach to the herpesvirus 'life' cycle

Tzviya Zeev-Ben-Mordehai; Christoph Hagen; Kay Gruenewald

doi:10.1016/j.coviro.2014.01.008

A cool hybrid approach to the herpesvirus 'life' cycle

Tzviya Zeev-Ben-Mordehai, Christoph Hagen, Kay Gruenewald

extern

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Electron cryo tomography (cryoET) is an ideal technique to study virus–host interactions at molecular resolution. Imaging of biological specimens in a frozen-hydrated state assures a close to native environment. Various virus–host cell interactions have been analysed in this way, with the herpesvirus ‘life’ cycle being the most comprehensively studied. The data obtained were further integrated with fluorescence and soft X-ray cryo microscopy data applied on experimental systems covering a wide range of biological complexity. This hybrid approach combines dynamic with static imaging and spans a resolution range from micrometres to angstroms. Along selected aspects of the herpesvirus replication cycle, we describe dedicated combinations of approaches and how subsequent data integration enables insights towards a functional understanding of the underlying processes.

Original language	English
Pages (from-to)	42-49
Number of pages	8
Journal	Current Opinion in Virology
Volume	5
DOIs	https://doi.org/10.1016/j.coviro.2014.01.008
Publication status	Published - Apr 2014
Externally published	Yes

Access to Document

10.1016/j.coviro.2014.01.008

1-s2.0-S1879625714000091-mainFinal published version, 2.77 MB

Cite this

@article{468387d651cf40f7bab6c57674f1f062,

title = "A cool hybrid approach to the herpesvirus 'life' cycle",

abstract = "Electron cryo tomography (cryoET) is an ideal technique to study virus–host interactions at molecular resolution. Imaging of biological specimens in a frozen-hydrated state assures a close to native environment. Various virus–host cell interactions have been analysed in this way, with the herpesvirus {\textquoteleft}life{\textquoteright} cycle being the most comprehensively studied. The data obtained were further integrated with fluorescence and soft X-ray cryo microscopy data applied on experimental systems covering a wide range of biological complexity. This hybrid approach combines dynamic with static imaging and spans a resolution range from micrometres to angstroms. Along selected aspects of the herpesvirus replication cycle, we describe dedicated combinations of approaches and how subsequent data integration enables insights towards a functional understanding of the underlying processes.",

author = "Tzviya Zeev-Ben-Mordehai and Christoph Hagen and Kay Gruenewald",

year = "2014",

month = apr,

doi = "10.1016/j.coviro.2014.01.008",

language = "English",

volume = "5",

pages = "42--49",

journal = "Current Opinion in Virology",

issn = "1879-6257",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - A cool hybrid approach to the herpesvirus 'life' cycle

AU - Zeev-Ben-Mordehai, Tzviya

AU - Hagen, Christoph

AU - Gruenewald, Kay

PY - 2014/4

Y1 - 2014/4

N2 - Electron cryo tomography (cryoET) is an ideal technique to study virus–host interactions at molecular resolution. Imaging of biological specimens in a frozen-hydrated state assures a close to native environment. Various virus–host cell interactions have been analysed in this way, with the herpesvirus ‘life’ cycle being the most comprehensively studied. The data obtained were further integrated with fluorescence and soft X-ray cryo microscopy data applied on experimental systems covering a wide range of biological complexity. This hybrid approach combines dynamic with static imaging and spans a resolution range from micrometres to angstroms. Along selected aspects of the herpesvirus replication cycle, we describe dedicated combinations of approaches and how subsequent data integration enables insights towards a functional understanding of the underlying processes.

AB - Electron cryo tomography (cryoET) is an ideal technique to study virus–host interactions at molecular resolution. Imaging of biological specimens in a frozen-hydrated state assures a close to native environment. Various virus–host cell interactions have been analysed in this way, with the herpesvirus ‘life’ cycle being the most comprehensively studied. The data obtained were further integrated with fluorescence and soft X-ray cryo microscopy data applied on experimental systems covering a wide range of biological complexity. This hybrid approach combines dynamic with static imaging and spans a resolution range from micrometres to angstroms. Along selected aspects of the herpesvirus replication cycle, we describe dedicated combinations of approaches and how subsequent data integration enables insights towards a functional understanding of the underlying processes.

U2 - 10.1016/j.coviro.2014.01.008

DO - 10.1016/j.coviro.2014.01.008

M3 - Article

SN - 1879-6257

VL - 5

SP - 42

EP - 49

JO - Current Opinion in Virology

JF - Current Opinion in Virology

ER -

A cool hybrid approach to the herpesvirus 'life' cycle

Abstract

Access to Document

Fingerprint

Cite this