Abstract
A purification procedure is described for the initiation factors of protein synthesis from rabbit reticulocytes: (a) from the ribosomal wash and (b) from the postribosomal supernantant. A comparison is made between these preparations with respect to yield and specific activity. eIF-4A and eIF-4D occur mainly in the postribosomal supernatant; eIF-2, eIF-4C and eIF-5 are more evenly divided over both fractions, whereas eIF-1, eIF-3 and eIF-4B are found almost exclusively in the ribosomal wash. No significant difference in specific activity could be detected when factors from both sources were compared, with a possible exception of eIF-4A and eIF-4D.
| Original language | English |
|---|---|
| Pages (from-to) | 329-37 |
| Number of pages | 9 |
| Journal | European Journal of Biochemistry |
| Volume | 98 |
| Issue number | 2 |
| Publication status | Published - 1 Aug 1979 |
Keywords
- Animals
- Cytosol
- Kinetics
- Molecular Weight
- Peptide Chain Initiation, Translational
- Peptide Initiation Factors
- Rabbits
- Reticulocytes
- Ribosomes