TY - JOUR
T1 - A chicory cytochrome P450 mono-oxygenase CYP71AV8 for the oxidation of (+)-valencene
AU - Cankar, K.
AU - van Houwelingen, A.
AU - Bosch, H.J.
AU - Sonke, T.
AU - Bouwmeester, H.
AU - Beekwilder, J.P.
PY - 2011/1/3
Y1 - 2011/1/3
N2 - Chicory (Cichorium intybus L.), which is known to have a variety of terpene-hydroxylating activities,
was screened for a P450 mono-oxygenase to convert (+)-valencene to (+)-nootkatone. A novel P450
cDNA was identified in a chicory root EST library. Co-expression of the enzyme with a valencene synthase
in yeast, led to formation of trans-nootkatol, cis-nootkatol and (+)-nootkatone. The novel
enzyme was also found to catalyse a three step conversion of germacrene A to germacra-
1(10),4,11(13)-trien-12-oic acid, indicating its involvement in chicory sesquiterpene lactone biosynthesis.
Likewise, amorpha-4,11-diene was converted to artemisinic acid. Surprisingly, the chicory
P450 has a different regio-specificity on (+)-valencene compared to germacrene A and amorpha-
4,11-diene.
AB - Chicory (Cichorium intybus L.), which is known to have a variety of terpene-hydroxylating activities,
was screened for a P450 mono-oxygenase to convert (+)-valencene to (+)-nootkatone. A novel P450
cDNA was identified in a chicory root EST library. Co-expression of the enzyme with a valencene synthase
in yeast, led to formation of trans-nootkatol, cis-nootkatol and (+)-nootkatone. The novel
enzyme was also found to catalyse a three step conversion of germacrene A to germacra-
1(10),4,11(13)-trien-12-oic acid, indicating its involvement in chicory sesquiterpene lactone biosynthesis.
Likewise, amorpha-4,11-diene was converted to artemisinic acid. Surprisingly, the chicory
P450 has a different regio-specificity on (+)-valencene compared to germacrene A and amorpha-
4,11-diene.
U2 - 10.1016/j.febslet.2010.11.040
DO - 10.1016/j.febslet.2010.11.040
M3 - Article
SN - 0014-5793
VL - 585
SP - 178
EP - 182
JO - FEBS Letters
JF - FEBS Letters
IS - 1
ER -