A 3D doubly sensitivity enhanced X-filtered TOCSY-TOCSY experiment

Hugo van Ingen; Marco Tessari; Geerten W. Vuister

doi:10.1023/A:1020975705237

A 3D doubly sensitivity enhanced X-filtered TOCSY-TOCSY experiment

Hugo van Ingen, Marco Tessari, Geerten W. Vuister

Radboud University Nijmegen

Research output: Contribution to journal › Review article › peer-review

Abstract

We present a 3D double sensitivity enhanced X-filtered homonuclear TOCSY-TOCSY experiment for the assignment of unlabeled molecules complexed to labeled protein- or nucleic acid-domains. The resulting spectrum is clean, can be measured in a reasonable amount of time and allows for increased resolution of overlapping resonances when compared to 2D methods. The 3D X-filtered TOCSY-TOCSY allows for assignment in cases where the size or the composition of the unlabeled molecule results in a high degree of overlap.

Original language	English
Pages (from-to)	155-160
Number of pages	6
Journal	Journal of Biomolecular NMR
Volume	24
Issue number	2
DOIs	https://doi.org/10.1023/A:1020975705237
Publication status	Published - 1 Oct 2002
Externally published	Yes

Keywords

Assignment
Complex
Double sensitivity enhancement
NMR
TOCSY
X-filter
binding protein
nucleic acid
protein Mad1
analytic method
complex formation
controlled study
filtration
intermethod comparison
isotope labeling
molecular size
nucleic acid analysis
priority journal
process optimization
protein domain
protein interaction
proton nuclear magnetic resonance
review
sensitivity analysis
time

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10.1023/A:1020975705237

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title = "A 3D doubly sensitivity enhanced X-filtered TOCSY-TOCSY experiment",

abstract = "We present a 3D double sensitivity enhanced X-filtered homonuclear TOCSY-TOCSY experiment for the assignment of unlabeled molecules complexed to labeled protein- or nucleic acid-domains. The resulting spectrum is clean, can be measured in a reasonable amount of time and allows for increased resolution of overlapping resonances when compared to 2D methods. The 3D X-filtered TOCSY-TOCSY allows for assignment in cases where the size or the composition of the unlabeled molecule results in a high degree of overlap.",

keywords = "Assignment, Complex, Double sensitivity enhancement, NMR, TOCSY, X-filter, binding protein, nucleic acid, protein Mad1, analytic method, complex formation, controlled study, filtration, intermethod comparison, isotope labeling, molecular size, nucleic acid analysis, priority journal, process optimization, protein domain, protein interaction, proton nuclear magnetic resonance, review, sensitivity analysis, time",

author = "\{van Ingen\}, Hugo and Marco Tessari and Vuister, \{Geerten W.\}",

year = "2002",

month = oct,

day = "1",

doi = "10.1023/A:1020975705237",

language = "English",

volume = "24",

pages = "155--160",

journal = "Journal of Biomolecular NMR",

issn = "0925-2738",

publisher = "Springer Science and Business Media B.V.",

number = "2",

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TY - JOUR

T1 - A 3D doubly sensitivity enhanced X-filtered TOCSY-TOCSY experiment

AU - van Ingen, Hugo

AU - Tessari, Marco

AU - Vuister, Geerten W.

PY - 2002/10/1

Y1 - 2002/10/1

N2 - We present a 3D double sensitivity enhanced X-filtered homonuclear TOCSY-TOCSY experiment for the assignment of unlabeled molecules complexed to labeled protein- or nucleic acid-domains. The resulting spectrum is clean, can be measured in a reasonable amount of time and allows for increased resolution of overlapping resonances when compared to 2D methods. The 3D X-filtered TOCSY-TOCSY allows for assignment in cases where the size or the composition of the unlabeled molecule results in a high degree of overlap.

AB - We present a 3D double sensitivity enhanced X-filtered homonuclear TOCSY-TOCSY experiment for the assignment of unlabeled molecules complexed to labeled protein- or nucleic acid-domains. The resulting spectrum is clean, can be measured in a reasonable amount of time and allows for increased resolution of overlapping resonances when compared to 2D methods. The 3D X-filtered TOCSY-TOCSY allows for assignment in cases where the size or the composition of the unlabeled molecule results in a high degree of overlap.

KW - Assignment

KW - Complex

KW - Double sensitivity enhancement

KW - NMR

KW - TOCSY

KW - X-filter

KW - binding protein

KW - nucleic acid

KW - protein Mad1

KW - analytic method

KW - complex formation

KW - controlled study

KW - filtration

KW - intermethod comparison

KW - isotope labeling

KW - molecular size

KW - nucleic acid analysis

KW - priority journal

KW - process optimization

KW - protein domain

KW - protein interaction

KW - proton nuclear magnetic resonance

KW - review

KW - sensitivity analysis

KW - time

U2 - 10.1023/A:1020975705237

DO - 10.1023/A:1020975705237

M3 - Review article

SN - 0925-2738

VL - 24

SP - 155

EP - 160

JO - Journal of Biomolecular NMR

JF - Journal of Biomolecular NMR

IS - 2

ER -

A 3D doubly sensitivity enhanced X-filtered TOCSY-TOCSY experiment

Abstract

Keywords

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