3D visualization of plant-pathogen interaction inside plant leaves with dynamic contrast optical coherence tomography

Jos de Wit; Sebastian Tonn; Mon Ray Shao; Guido Van den Ackerveken; Jeroen Kalkman

3D visualization of plant-pathogen interaction inside plant leaves with dynamic contrast optical coherence tomography

Jos de Wit, Sebastian Tonn, Mon Ray Shao, Guido Van den Ackerveken, Jeroen Kalkman^*

^*Corresponding author for this work

Delft University of Technology

Research output: Contribution to conference › Paper › Academic

Abstract

Optical coherence tomography (OCT) can image deep inside scattering plant tissue with micrometer resolution. However, conventional OCT lacks specificity to distinguish plant tissue from pathogen tissue. Here we show how dynamic OCT (dOCT) creates functional contrast of Bremia, a downy mildew in lettuce, based on sub-resolution dynamic activity inside the tissue. We demonstrate its applicability for disease resistance quantification and longitudinal study of pathogen growth.

Original language	English
Publication status	Published - 19 Jul 2024
Event	3D Image Acquisition and Display: Technology, Perception and Applications, 3D 2024 - Part of Optica Imaging Congress - Toulouse, France Duration: 15 Jul 2024 → 19 Jul 2024

Conference

Conference	3D Image Acquisition and Display: Technology, Perception and Applications, 3D 2024 - Part of Optica Imaging Congress
Country/Territory	France
City	Toulouse
Period	15/07/24 → 19/07/24

Access to Document

https://opg.optica.org/abstract.cfm?uri=pcAOP-2024-JTh2A.16

Cite this

de Wit, J., Tonn, S., Shao, M. R., Van den Ackerveken, G., & Kalkman, J. (2024). 3D visualization of plant-pathogen interaction inside plant leaves with dynamic contrast optical coherence tomography. Paper presented at 3D Image Acquisition and Display: Technology, Perception and Applications, 3D 2024 - Part of Optica Imaging Congress, Toulouse, France. https://opg.optica.org/abstract.cfm?uri=pcAOP-2024-JTh2A.16

@conference{a97fd2781b1a464c8cf9a4ed9fdf399e,

title = "3D visualization of plant-pathogen interaction inside plant leaves with dynamic contrast optical coherence tomography",

abstract = "Optical coherence tomography (OCT) can image deep inside scattering plant tissue with micrometer resolution. However, conventional OCT lacks specificity to distinguish plant tissue from pathogen tissue. Here we show how dynamic OCT (dOCT) creates functional contrast of Bremia, a downy mildew in lettuce, based on sub-resolution dynamic activity inside the tissue. We demonstrate its applicability for disease resistance quantification and longitudinal study of pathogen growth.",

author = "{de Wit}, Jos and Sebastian Tonn and Shao, {Mon Ray} and {Van den Ackerveken}, Guido and Jeroen Kalkman",

note = "Publisher Copyright: {\textcopyright} 2024 The Author(s).; 3D Image Acquisition and Display: Technology, Perception and Applications, 3D 2024 - Part of Optica Imaging Congress ; Conference date: 15-07-2024 Through 19-07-2024",

year = "2024",

month = jul,

day = "19",

language = "English",

}

de Wit, J, Tonn, S, Shao, MR, Van den Ackerveken, G & Kalkman, J 2024, '3D visualization of plant-pathogen interaction inside plant leaves with dynamic contrast optical coherence tomography', Paper presented at 3D Image Acquisition and Display: Technology, Perception and Applications, 3D 2024 - Part of Optica Imaging Congress, Toulouse, France, 15/07/24 - 19/07/24. <https://opg.optica.org/abstract.cfm?uri=pcAOP-2024-JTh2A.16>

3D visualization of plant-pathogen interaction inside plant leaves with dynamic contrast optical coherence tomography. / de Wit, Jos; Tonn, Sebastian; Shao, Mon Ray et al.
2024. Paper presented at 3D Image Acquisition and Display: Technology, Perception and Applications, 3D 2024 - Part of Optica Imaging Congress, Toulouse, France.

Research output: Contribution to conference › Paper › Academic

TY - CONF

T1 - 3D visualization of plant-pathogen interaction inside plant leaves with dynamic contrast optical coherence tomography

AU - de Wit, Jos

AU - Tonn, Sebastian

AU - Shao, Mon Ray

AU - Van den Ackerveken, Guido

AU - Kalkman, Jeroen

PY - 2024/7/19

Y1 - 2024/7/19

N2 - Optical coherence tomography (OCT) can image deep inside scattering plant tissue with micrometer resolution. However, conventional OCT lacks specificity to distinguish plant tissue from pathogen tissue. Here we show how dynamic OCT (dOCT) creates functional contrast of Bremia, a downy mildew in lettuce, based on sub-resolution dynamic activity inside the tissue. We demonstrate its applicability for disease resistance quantification and longitudinal study of pathogen growth.

AB - Optical coherence tomography (OCT) can image deep inside scattering plant tissue with micrometer resolution. However, conventional OCT lacks specificity to distinguish plant tissue from pathogen tissue. Here we show how dynamic OCT (dOCT) creates functional contrast of Bremia, a downy mildew in lettuce, based on sub-resolution dynamic activity inside the tissue. We demonstrate its applicability for disease resistance quantification and longitudinal study of pathogen growth.

UR - http://www.scopus.com/inward/record.url?scp=85204917564&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:85204917564

T2 - 3D Image Acquisition and Display: Technology, Perception and Applications, 3D 2024 - Part of Optica Imaging Congress

Y2 - 15 July 2024 through 19 July 2024

ER -

3D visualization of plant-pathogen interaction inside plant leaves with dynamic contrast optical coherence tomography

Abstract

Conference

Access to Document

Fingerprint

Cite this