TY - JOUR
T1 - 3D morphometry of endothelial cells angiogenesis in an extracellular matrix composite hydrogel
AU - Heidari, Faranak
AU - Shamshiripour, Parisa
AU - Rahnama, Mehrana
AU - Saadatmand, Maryam
AU - Ahmadvand, Davoud
AU - Simorgh, Sara
AU - Moradi, Ali Reza
N1 - Publisher Copyright:
© 2024 The Author(s)
PY - 2024/11/15
Y1 - 2024/11/15
N2 - Human umbilical vein endothelial cells (HUVECs) play a fundamental role in angiogenesis. Herein, we introduce digital holographic microscopy (DHM) for the 3D quantitative morphological analysis of HUVECs in extracellular matrix (ECM)-based biomaterials as an angiogenesis model. The combination of volumetric information from DHM and the physicochemical and cytobiocompatibility data provided by fluorescence microscopy and cytology offers a comprehensive understanding of the angiogenesis-related parameters of HUVECs within the ECM. DHM enables label-free, non-contact, and non-invasive 3D monitoring of living samples in real time, in a quantitative manner. In this study, the human amniotic membrane (HAM) is decellularized, pulverized, and combined with sodium alginate hydrogel to provide an in vitro substrate for modeling HUVEC angiogenesis. Our results demonstrate that modifying alginate hydrogel with HAM enhances its biofunctionality due to the presence of ECM components. Moreover, the DHM results reveal an increase in its porous properties, which, in turn, aids in interpreting the tubulation results.
AB - Human umbilical vein endothelial cells (HUVECs) play a fundamental role in angiogenesis. Herein, we introduce digital holographic microscopy (DHM) for the 3D quantitative morphological analysis of HUVECs in extracellular matrix (ECM)-based biomaterials as an angiogenesis model. The combination of volumetric information from DHM and the physicochemical and cytobiocompatibility data provided by fluorescence microscopy and cytology offers a comprehensive understanding of the angiogenesis-related parameters of HUVECs within the ECM. DHM enables label-free, non-contact, and non-invasive 3D monitoring of living samples in real time, in a quantitative manner. In this study, the human amniotic membrane (HAM) is decellularized, pulverized, and combined with sodium alginate hydrogel to provide an in vitro substrate for modeling HUVEC angiogenesis. Our results demonstrate that modifying alginate hydrogel with HAM enhances its biofunctionality due to the presence of ECM components. Moreover, the DHM results reveal an increase in its porous properties, which, in turn, aids in interpreting the tubulation results.
UR - http://www.scopus.com/inward/record.url?scp=85207156891&partnerID=8YFLogxK
U2 - 10.1016/j.heliyon.2024.e39616
DO - 10.1016/j.heliyon.2024.e39616
M3 - Article
AN - SCOPUS:85207156891
SN - 2405-8440
VL - 10
JO - Heliyon
JF - Heliyon
IS - 21
M1 - e39616
ER -