2'-fucosyllactose prevents rotavirus diarrhoea in suckling rats by preventing microbiota alteration and boosting toll-like receptors

I. Azagra Boronat, M. Castell Escuer, K. Knipping, J. Garssen, M. Rodriguez Lagunas, F. Pérez-Cano

Research output: Contribution to journalMeeting AbstractAcademic


Human milk oligosaccharides are involved in early life intestinal development, by influencing the intestinal microbiota and the immune system, among others (1). The most abundant oligosaccharide, 2'fucosyllactose (2'FL), represents ~30% of the total in human milk and has shown potential to provide protection against rotavirus (RV) infection. The aim of this work was to investigate the impact of 2'FL on microbiota-host crosstalk and gut microbiota composition during RV-induced diarrhoea in suckling rats. Lewis rats were daily administered with 2'FL from days 2-16 of life and on day 5 the RV was administered intragastrically. Faecal samples were collected daily for clinical evaluation of diarrhoea. On day 8, corresponding to the peak of diarrhoea, half of the animals were sacrificed in order to assess the intestinal Tolllike receptors (TLRs) expression. On day 16, the faecal microbiota composition was assessed by 16S rRNA sequencing and the caecal short-chain fatty acids (SCFAs) were quantified by HS-GCMS. 2'FL supplementation reduced severity, incidence and duration of diarrhoea. RV infection increased intestinal TLR2 expression and altered intestinal microbiota composition, by reducing several genera, such as Staphylococcus and Streptococcus, and promoting the colonization with Proteobacteria. Moreover, RV reduced caecal SCFA. However, the supplementation with 2'FL boosted TLR5 and TLR7, prevented the RV-induced microbiota alteration, promoted the colonization of new species and increased the caecal SCFA butyrate. The supplementation with 2'FL in early life ameliorates RV diarrhoea. The increased TLRs expression and the prevention of RVinduced microbiota alteration are suggested to be part of mechanisms involved in such amelioration.
Original languageEnglish
Pages (from-to)216-217
Number of pages2
JournalAnnals of Nutrition and Metabolism
Issue number3
Publication statusPublished - 9 Mar 2018


  • 2'-fucosyllactose
  • Diarrhoea
  • Microbiota
  • Rotavirus
  • TLR
  • endogenous compound
  • RNA 16S
  • short chain fatty acid
  • toll like receptor
  • toll like receptor 2
  • toll like receptor 5
  • toll like receptor 7
  • unclassified drug
  • animal experiment
  • animal model
  • clinical evaluation
  • conference abstract
  • controlled study
  • diarrhea
  • feces microflora
  • incidence
  • intestine flora
  • intragastric drug administration
  • Lewis rat
  • new species
  • nonhuman
  • prevention
  • protein expression
  • Proteobacteria
  • quantitative analysis
  • rat
  • Rotavirus infection
  • Staphylococcus
  • Streptococcus
  • suckling animal


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