1H NMR study of the exchangeable protons of the duplex d(GCGTTGCG).d(CGCAACGC) containing a thymine photodimer.

J. Kemmink; R. Boelens; T. Koning; G.A. van der Marel; J.H. van Boom; R. Kaptein

1H NMR study of the exchangeable protons of the duplex d(GCGTTGCG).d(CGCAACGC) containing a thymine photodimer.

J. Kemmink, R. Boelens, T. Koning, G.A. van der Marel, J.H. van Boom, R. Kaptein

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Abstract

A comparison is presented of the imino proton NMR spectra of the double stranded octamer d(GCGTTGCG).d(CGCAACGC) and the same octamer in which the two central thymine residues occur as a cis-syn thymine dimer. Except for the terminal base pairs all imino protons were detected and assigned in the NMR spectrum. The spectra show that in the thymine dimer duplex, contrary to common belief, all base pairs occur in a hydrogen bonded form, although the hydrogen bonds of the two central AT base pairs are substantially weakened. The melting temperature decreases about 13 degrees C on thymine dimer formation.

Original language	English
Pages (from-to)	4645-4653
Number of pages	9
Journal	Nucleic Acids Research
Volume	15
Issue number	11
Publication status	Published - 1987
Externally published	Yes

Keywords

imine
oligodeoxyribonucleotide
proton
pyrimidine dimer
article
conformation
DNA denaturation
nuclear magnetic resonance spectroscopy
synthesis
temperature

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@article{59c7a9e3139040ceae313099d1a80270,

title = "1H NMR study of the exchangeable protons of the duplex d(GCGTTGCG).d(CGCAACGC) containing a thymine photodimer.",

abstract = "A comparison is presented of the imino proton NMR spectra of the double stranded octamer d(GCGTTGCG).d(CGCAACGC) and the same octamer in which the two central thymine residues occur as a cis-syn thymine dimer. Except for the terminal base pairs all imino protons were detected and assigned in the NMR spectrum. The spectra show that in the thymine dimer duplex, contrary to common belief, all base pairs occur in a hydrogen bonded form, although the hydrogen bonds of the two central AT base pairs are substantially weakened. The melting temperature decreases about 13 degrees C on thymine dimer formation.",

keywords = "imine, oligodeoxyribonucleotide, proton, pyrimidine dimer, article, conformation, DNA denaturation, nuclear magnetic resonance spectroscopy, synthesis, temperature",

author = "J. Kemmink and R. Boelens and T. Koning and {van der Marel}, G.A. and {van Boom}, J.H. and R. Kaptein",

year = "1987",

language = "English",

volume = "15",

pages = "4645--4653",

journal = "Nucleic Acids Research",

issn = "0305-1048",

publisher = "Oxford University Press",

number = "11",

}

TY - JOUR

T1 - 1H NMR study of the exchangeable protons of the duplex d(GCGTTGCG).d(CGCAACGC) containing a thymine photodimer.

AU - Kemmink, J.

AU - Boelens, R.

AU - Koning, T.

AU - van der Marel, G.A.

AU - van Boom, J.H.

AU - Kaptein, R.

PY - 1987

Y1 - 1987

N2 - A comparison is presented of the imino proton NMR spectra of the double stranded octamer d(GCGTTGCG).d(CGCAACGC) and the same octamer in which the two central thymine residues occur as a cis-syn thymine dimer. Except for the terminal base pairs all imino protons were detected and assigned in the NMR spectrum. The spectra show that in the thymine dimer duplex, contrary to common belief, all base pairs occur in a hydrogen bonded form, although the hydrogen bonds of the two central AT base pairs are substantially weakened. The melting temperature decreases about 13 degrees C on thymine dimer formation.

AB - A comparison is presented of the imino proton NMR spectra of the double stranded octamer d(GCGTTGCG).d(CGCAACGC) and the same octamer in which the two central thymine residues occur as a cis-syn thymine dimer. Except for the terminal base pairs all imino protons were detected and assigned in the NMR spectrum. The spectra show that in the thymine dimer duplex, contrary to common belief, all base pairs occur in a hydrogen bonded form, although the hydrogen bonds of the two central AT base pairs are substantially weakened. The melting temperature decreases about 13 degrees C on thymine dimer formation.

KW - imine

KW - oligodeoxyribonucleotide

KW - proton

KW - pyrimidine dimer

KW - article

KW - conformation

KW - DNA denaturation

KW - nuclear magnetic resonance spectroscopy

KW - synthesis

KW - temperature

M3 - Article

SN - 0305-1048

VL - 15

SP - 4645

EP - 4653

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 11

ER -

1H NMR study of the exchangeable protons of the duplex d(GCGTTGCG).d(CGCAACGC) containing a thymine photodimer.

Abstract

Keywords

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